A Reliable Targeted Next-Generation Sequencing Strategy for Diagnosis of Myopathies and Muscular Dystrophies, Especially for the Giant Titin and Nebulin Genes [PDF]
, 2018 Reda Zenagui +22 more
openalex +1 more source
Peptide‐based ligand antagonists block a Vibrio cholerae adhesin
FEBS Letters, EarlyView.The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang +9 more
wiley +1 more source
Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline
International Journal of Medical Sciences, 2004 The theoretical possibility of applying gene transfer methodologies to the human germline is explored. Transgenic methods for genetically manipulating embryos may in principle be applied to humans.
doaj
Microglia targeting by adeno-associated viral vectors
Frontiers in ImmunologyMicroglia play a crucial role in maintaining homeostasis of the central nervous system and they are actively involved in shaping the brain’s inflammatory response to stress.
Maria Stamataki +3 more
doaj +1 more source
Detection and quantification of Listeria monocytogenes by 5'-nuclease polymerase chain reaction targeting the actA gene [PDF]
, 2005 Katarína Oravcová +6 more
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Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements. [PDF]
, 2019 Pooled CRISPR-Cas9 screens are a powerful method for functionally characterizing regulatory elements in the non-coding genome, but off-target effects in these experiments have not been systematically evaluated.
Aradhana +20 more
core
FEBS Letters, EarlyView.This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley +1 more source
Programmed cell death 6, a novel p53‐responsive gene, targets to the nucleus in the apoptotic response to
, 2012 K. Suzuki +5 more
openalex +1 more source
In silico Single-Cell Analysis of Steroid-Responsive Gene Targets in the Mammalian Cochlea
, 2022 Lacey Nelson +6 more
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Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing
FEBS Letters, EarlyView.Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley +1 more source