Results 351 to 360 of about 12,501,640 (402)
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2003
Gene targeting in mouse ES cells is a powerful method for studying gene function in vivo. For the novice, this combination of molecular biology, specialized tissue-culture cell lines, and mouse reproductive biology can be daunting. We present a straightforward, one might say constrained, guide for novices of gene targeting to generate a null allele in ...
Richard R. Behringer, Siew-Sim Cheah
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Gene targeting in mouse ES cells is a powerful method for studying gene function in vivo. For the novice, this combination of molecular biology, specialized tissue-culture cell lines, and mouse reproductive biology can be daunting. We present a straightforward, one might say constrained, guide for novices of gene targeting to generate a null allele in ...
Richard R. Behringer, Siew-Sim Cheah
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Altering Genes in Animals by Gene Targeting
Annual Review of Immunology, 1992It is possible to create mice in which a planned alteration has been introduced at a specific locus in the genome. The planned alteration is first introduced into the genome of mouse embryonic stem (ES) cells in tissue culture. This is accomplished by homologous recombination between the targeted genomic locus and DNA introduced into the ES cells ...
Oliver Smithies, Beverly H. Koller
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Targeted gene regulation and gene ablation
Trends in Endocrinology & Metabolism, 2001Investigations of the mechanisms involved in appropriate, developmentally regulated tissue-specific gene transcription have laid the foundations for transgenic and gene-therapy technologies directing specific induction or ablation of genes of interest in a tissue-restricted manner.
Sophia Y. Tsai, Francesco J. DeMayo
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Targeting Trichothecene Biosynthetic Genes [PDF]
, 2016 Biosynthesis of trichothecenes requires the involvement of at least 15 genes, most of which have been targeted for PCR. Qualitative PCRs are used to assign chemotypes to individual isolates, e.g., the capacity to produce type A and/or type B trichothecenes. Many regions in the core cluster (consisting of 12 genes) including intergenic regions have been
Wei, Songhong +4 more
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BioEssays, 1994
AbstractMice with alterations to specific endogenous genes can be produced by gene targeting in embryonic stem cells. The field has developed rapidly over the past decade, so that large numbers of mice with different gene deficiencies have been generated.
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AbstractMice with alterations to specific endogenous genes can be produced by gene targeting in embryonic stem cells. The field has developed rapidly over the past decade, so that large numbers of mice with different gene deficiencies have been generated.
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Antibodies for targeted gene therapy: extracellular gene targeting and intracellular expression
Advanced Drug Delivery Reviews, 1998Antibody genes of human origin and human antibodies directed against human proteins have become widely available in recent years. These are valuable reagents for gene therapy applications, in which the use of human proteins and genes allows for increased therapeutic benefit. Engineered human antibodies can be used in gene therapy both as a component of
Wayne A. Marasco, Susan Dana Jones
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Gene Targeting for Gene Therapy: Prospects
Molecular Genetics and Metabolism, 1999Ideally, gene therapy involves the correction of genetic defects through the natural means of gene targeting. This therapy possesses a number of conceptual advantages. However, a major obstacle to successful gene therapy is the relative inefficiency of the targeting process in mammalian cells.
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Advances in Targeted Gene Delivery
Current Drug Delivery, 2019Gene therapy has the potential to treat both acquired and inherited genetic diseases. Generally, two types of gene delivery vectors are used - viral vectors and non-viral vectors. Non-viral gene delivery systems have attracted significant interest (e.g.
Ara Begum, Anjuman +3 more
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Gene targeting in livestock: a preview
Transgenic Research, 2000Until recently genetically modified livestock could only be generated by pronuclear injection. The discovery that animals can be cloned by nuclear transfer from cultured somatic cells means that it will now be possible to achieve gene targeting in these species.
Sarah Burl +3 more
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