Results 51 to 60 of about 708,347 (229)
Transposon-mediated generation of targeting vectors for the production of gene knockouts [PDF]
, 2005 Vectors used for gene targeting experiments usually consist of a selectable marker flanked by two regions of homology to the targeted gene. In a homologous recombination event, the selectable marker replaces an essential element of the target gene ...
Chy, Hun +4 more
core +4 more sources
Frontiers in Genome Editing, 2021 Gene targeting (GT) enables precise genome modification—e.g., the introduction of base substitutions—using donor DNA as a template. Combined with clean excision of the selection marker used to select GT cells, GT is expected to become a standard ...
Namie Ohtsuki +11 more
doaj +1 more source
Efficient Gene Targeting Mediated by Adeno-Associated Virus and DNA Double-Strand Breaks [PDF]
, 2003 Gene targeting is the in situ manipulation of the sequence of an endogenous gene by the introduction of homologous exogenous DNA. Presently, the rate of gene targeting is too low for it to be broadly used in mammalian somatic cell genetics or to cure ...
Baltimore, David +3 more
core
Specific genetic modifications of domestic animals by gene targeting and animal cloning [PDF]
, 2003 The technology of gene targeting through homologous recombination has been extremely useful for elucidating gene functions in mice. The application of this technology was thought impossible in the large livestock species until the successful creation of ...
Wang, Bin, Zhou, Jiangfeng
core +3 more sources
Molecular Systems Biology, 2009 The molecular complexity of mammalian proteomes demands new methods for mapping the organization of multiprotein complexes. Here, we combine mouse genetics and proteomics to characterize synapse protein complexes and interaction networks.
Esperanza Fernández +9 more
doaj +1 more source
Novel Approach in the Construction of Bioethanol-Producing Saccharomyces cerevisiae Hybrids
Food Technology and Biotechnology, 2019 Bioethanol production from lignocellulosic hydrolysates requires a producer strain that tolerates both the presence of growth and fermentation inhibitors and high ethanol concentrations. Therefore, we constructed heterozygous intraspecies hybrid diploids
Anamarija Štafa +6 more
doaj +1 more source
Targeted Gene Editing in Porcine Spermatogonia
Frontiers in Genetics, 2021 To study the pathophysiology of human diseases, develop innovative treatments, and refine approaches for regenerative medicine require appropriate preclinical models.
Dennis Webster +7 more
doaj +1 more source
Identifying Stress Responsive Genes using Overlapping Communities in Co-expression Networks [PDF]
, 2020 This paper proposes a workflow to identify genes that respond to specific treatments in plants. The workflow takes as input the RNA sequencing read counts and phenotypical data of different genotypes, measured under control and treatment conditions. It outputs a reduced group of genes marked as relevant for treatment response. Technically, the proposed
arxiv +1 more source
Advances in Delivery Mechanisms of CRISPR Gene-Editing Reagents in Plants
Frontiers in Genome Editing, 2022 Gene-editing by CRISPR/Cas systems has revolutionized plant biology by serving as a functional genomics tool. It has tremendously advanced plant breeding and crop improvement by accelerating the development of improved cultivars, creating genetic ...
Larissa C. Laforest +2 more
doaj +1 more source
Use of integrase-minus lentiviral vector for transient expression [PDF]
, 2012 Objective: Lentivirus-derived vectors are among the most promising viral vectors for gene therapy which is currently available, but their use in clinical practice is limited due to associated risk of insertional mutagenesis.
Azadeh, H. +5 more
core +2 more sources