Results 31 to 40 of about 1,132,364 (264)

Targeted Forward Genetics: Population-Scale Analyses of Allele Replacements Spanning Thousands of Base Pairs in Fission Yeast

G3: Genes, Genomes, Genetics, 2019
Precise allele replacement (genome editing), without unwanted changes to the genome, provides a powerful tool to define the functions of DNA elements and encoded factors in their normal biological context.
Aaron J. Storey, Hsin-Ping Wang, Reine U. Protacio, Mari K. Davidson, Wayne P. Wahls   +4 more
doaj   +1 more source

Enhancing HR Frequency for Precise Genome Editing in Plants

Frontiers in Plant Science, 2022
Gene-editing tools, such as Zinc-fingers, TALENs, and CRISPR-Cas, have fostered a new frontier in the genetic improvement of plants across the tree of life.
Hao Chen, Hao Chen, Matthew Neubauer, Jack P. Wang, Jack P. Wang   +4 more
doaj   +1 more source

Molecular bases of low production rates of apolipoprotein B-100 and truncated apoB-82 in a mutant HepG2 cell line generated by targeted modification of the apolipoprotein B gene

Journal of Lipid Research, 1999
In subjects with familial hypobetalipoproteinemia heterozygous for truncated forms of apolipoprotein B, both apoB-100 and the truncated forms are produced at lower than expected rates.
Rai Ajit K. Srivastava, Neelam Srivastava, Maurizio Averna, Angelo B. Cefalu, Gustav Schonfeld   +4 more
doaj   +1 more source

Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay

Frontiers in Microbiology, 2018
Lipophosphoglycan (LPG) is the major surface glycoconjugate of metacyclic Leishmania promastigotes and is associated with virulence in various species of this parasite.
Milena Lázaro-Souza, Milena Lázaro-Souza, Christine Matte, Jonilson B. Lima, Guillermo Arango Duque, Graziele Quintela-Carvalho, Graziele Quintela-Carvalho, Graziele Quintela-Carvalho, Áislan de Carvalho Vivarini, Sara Moura-Pontes, Cláudio P. Figueira, Flávio H. Jesus-Santos, Flávio H. Jesus-Santos, Ulisses Gazos Lopes, Leonardo P. Farias, Théo Araújo-Santos, Albert Descoteaux, Valéria M. Borges, Valéria M. Borges   +18 more
doaj   +1 more source

Targeting in Gene Therapy for Gliomas [PDF]

Archives of Neurology, 1999
Cancer is a disease of a series of genes. Thus, theoretically, brain tumors could be treated by targeting their fundamental molecular defects. Currently, most of the approved clinical protocols for gene therapy involve cancer patients. Several of these protocols are designed to improve the treatment of brain tumors. In this brief report, we analyze the
Fueyo, J., Gomez-Manzano, C., Yung, W. K., Kyritsis, A. P.   +3 more
openaire   +3 more sources

In vivo armed macrophages curb liver metastasis through tumor-reactive T-cell rejuvenation

Nature Communications
Despite recent progress in cancer treatment, liver metastases persist as an unmet clinical need. Here, we show that arming liver and tumor-associated macrophages in vivo to co-express tumor antigens (TAs), IFNα, and IL-12 unleashes robust anti-tumor ...
Marco Notaro, Maristella Borghetti, Chiara Bresesti, Giovanna Giacca, Thomas Kerzel, Carl Mirko Mercado, Stefano Beretta, Marco Monti, Ivan Merelli, Silvia Iaia, Marco Genua, Andrea Annoni, Tamara Canu, Patrizia Cristofori, Sara Degl’Innocenti, Francesca Sanvito, Paola Maria Vittoria Rancoita, Renato Ostuni, Silvia Gregori, Luigi Naldini, Mario Leonardo Squadrito   +20 more
doaj   +1 more source

European Standard Clinical Practice Guideline and EXPeRT Recommendations for the Diagnosis and Management of Gastroenteropancreatic Neuroendocrine Neoplasms in Children and Adolescents

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric gastroenteropancreatic neuroendocrine neoplasms (GEP‐NENs) are extremely rare and clinically heterogeneous. Management has largely been extrapolated from adult practice. This European Standard Clinical Practice Guideline (ESCP), developed by the EXPeRT network in collaboration with adult NEN experts, provides (adult) evidence ...
Michaela Kuhlen, Calogero Virgone, Antje Redlich, Michael Abele, Ricardo Lopez‐Almaraz, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Maja Mazic Cesen, Andrea Ferrari, Malgorzata A. Krawczyk, Yves Reguerre, Dominik T. Schneider, Ines B. Brecht, Charlotte Rigaud, Bilgehan Yalcin, Rainer Claus, Christian Vokuhl, Constantin Lapa, Jörg Fuchs, Segolene Hescot, Thomas Walter, Julien Hadoux, Daniel Orbach   +23 more
wiley   +1 more source

Targeted tandem affinity purification of PSD‐95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins

Molecular Systems Biology, 2009
The molecular complexity of mammalian proteomes demands new methods for mapping the organization of multiprotein complexes. Here, we combine mouse genetics and proteomics to characterize synapse protein complexes and interaction networks.
Esperanza Fernández, Mark O Collins, Rachel T Uren, Maksym V Kopanitsa, Noboru H Komiyama, Mike D R Croning, Lysimachos Zografos, J Douglas Armstrong, Jyoti S Choudhary, Seth G N Grant   +9 more
doaj   +1 more source

Novel Heterotypic Rox Sites for Combinatorial Dre Recombination Strategies

G3: Genes, Genomes, Genetics, 2016
Site-specific recombinases (SSRs) such as Cre are widely used in gene targeting and genetic approaches for cell labeling and manipulation. They mediate DNA strand exchange between two DNA molecules at dedicated recognition sites. Precise understanding of
Katherine Chuang, Eileen Nguyen, Yuri Sergeev, Tudor C. Badea   +3 more
doaj   +1 more source

iPSC-Derived Intestinal Organoids from Cystic Fibrosis Patients Acquire CFTR Activity upon TALEN-Mediated Repair of the p.F508del Mutation

Molecular Therapy: Methods & Clinical Development, 2020
Cystic fibrosis (CF) is the main genetic cause of death among the Caucasian population. The disease is characterized by abnormal fluid and electrolyte mobility across secretory epithelia.
Aarne Fleischer, Sara Vallejo-Díez, José María Martín-Fernández, Almudena Sánchez-Gilabert, Mónica Castresana, Angel del Pozo, Amaia Esquisabel, Silvia Ávila, José Luis Castrillo, Eusebio Gaínza, José Luis Pedraz, Miguel Viñas, Daniel Bachiller   +12 more
doaj   +1 more source