Results 31 to 40 of about 1,734,183 (310)

Targeted Forward Genetics: Population-Scale Analyses of Allele Replacements Spanning Thousands of Base Pairs in Fission Yeast

G3: Genes, Genomes, Genetics, 2019
Precise allele replacement (genome editing), without unwanted changes to the genome, provides a powerful tool to define the functions of DNA elements and encoded factors in their normal biological context.
Aaron J. Storey, Hsin-Ping Wang, Reine U. Protacio, Mari K. Davidson, Wayne P. Wahls   +4 more
doaj   +1 more source

Enhancing HR Frequency for Precise Genome Editing in Plants

Frontiers in Plant Science, 2022
Gene-editing tools, such as Zinc-fingers, TALENs, and CRISPR-Cas, have fostered a new frontier in the genetic improvement of plants across the tree of life.
Hao Chen, Hao Chen, Matthew Neubauer, Jack P. Wang, Jack P. Wang   +4 more
doaj   +1 more source

Molecular bases of low production rates of apolipoprotein B-100 and truncated apoB-82 in a mutant HepG2 cell line generated by targeted modification of the apolipoprotein B gene

Journal of Lipid Research, 1999
In subjects with familial hypobetalipoproteinemia heterozygous for truncated forms of apolipoprotein B, both apoB-100 and the truncated forms are produced at lower than expected rates.
Rai Ajit K. Srivastava, Neelam Srivastava, Maurizio Averna, Angelo B. Cefalu, Gustav Schonfeld   +4 more
doaj   +1 more source

Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]

, 2015
Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas, LaFayette, Peter R, Parrott, Wayne A, Schmitz, Robert J   +3 more
core   +2 more sources

Primer selection impacts specific population abundances but not community dynamics in a monthly time-series 16S rRNA gene amplicon analysis of coastal marine bacterioplankton. [PDF]

, 2018
Primers targeting the 16S small subunit ribosomal RNA marker gene, used to characterize bacterial and archaeal communities, have recently been re-evaluated for marine planktonic habitats.
Carlson, Craig A, Nelson, Craig E, Wear, Emma K, Wilbanks, Elizabeth G   +3 more
core   +1 more source

CRISPR-Cas9-mediated functional dissection of 3'-UTRs. [PDF]

, 2017
Many studies using reporter assays have demonstrated that 3' untranslated regions (3'-UTRs) regulate gene expression by controlling mRNA stability and translation.
Ahituv, Nadav, Biton, Anne, Erle, David J, Siegel, David, Tonqueze, Olivier Le, Zaitlen, Noah, Zhao, Wenxue   +6 more
core   +1 more source

Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae [PDF]

, 2020
The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group ...
Dermauw, Wannes, Jonckheere, Wim, Livadaras, Ioannis, Riga, Maria, Van Leeuwen, Thomas, Vontas, John   +5 more
core   +1 more source

Gene targeting in Arabidopsis

The Plant Journal, 2001
Summary Precise modification by gene targeting (GT) provides an important tool for studies of gene function in vivo . Although routine with many organisms, only isolated examples of GT events have been reported for flowering plants.
Hanin, M., Volrath, S., Bogucki, A., Briker, M., Ward, E., Paszkowski, Jerzy   +5 more
openaire   +4 more sources

Leishmania infantum Lipophosphoglycan-Deficient Mutants: A Tool to Study Host Cell-Parasite Interplay

Frontiers in Microbiology, 2018
Lipophosphoglycan (LPG) is the major surface glycoconjugate of metacyclic Leishmania promastigotes and is associated with virulence in various species of this parasite.
Milena Lázaro-Souza, Milena Lázaro-Souza, Christine Matte, Jonilson B. Lima, Guillermo Arango Duque, Graziele Quintela-Carvalho, Graziele Quintela-Carvalho, Graziele Quintela-Carvalho, Áislan de Carvalho Vivarini, Sara Moura-Pontes, Cláudio P. Figueira, Flávio H. Jesus-Santos, Flávio H. Jesus-Santos, Ulisses Gazos Lopes, Leonardo P. Farias, Théo Araújo-Santos, Albert Descoteaux, Valéria M. Borges, Valéria M. Borges   +18 more
doaj   +1 more source

Verification of DNA motifs in Arabidopsis using CRISPR/Cas9-mediated mutagenesis. [PDF]

, 2018
Transcription factors (TFs) and chromatin-modifying factors (CMFs) access chromatin by recognizing specific DNA motifs in their target genes. Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) has been widely used to discover
Chen, Chen, Chen, Huhui, Chen, Xuemei, Cui, Yuhai, Li, Chenlong, Wang, Suikang   +5 more
core   +2 more sources