Results 61 to 70 of about 1,717,717 (307)

Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi. [PDF]

, 2015
Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches ...
Dowdy, Steven F, Kaulich, Manuel, Lee, Yeon J, Lönn, Peter, Meade, Bryan R, Springer, Aaron D   +5 more
core   +1 more source

CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]

, 2020
The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I, Jacinto, Filipe, Link, Wolfgang   +2 more
core   +1 more source

MYCN Amplification in RB1‐Inactivated Retinoblastoma: Association With High‐Risk Features

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background MYCN amplification occurs in a subset of retinoblastoma cases, both with and without RB1 inactivation. It has been suggested that retinoblastomas with MYCN amplification represent a distinct entity with more aggressive clinical behavior.
Kyriaki Papaioannou, Regina Kubica, Karen Fischhuber, Jasmin Beygo, Madlen Reschke, Petra Ritter‐Sovinz, Eva Biewald, Petra Ketteler, Dietmar Lohmann, Deniz Kanber   +9 more
wiley   +1 more source

Multiple Gene Transfer and All-In-One Conditional Knockout Systems in Mouse Embryonic Stem Cells for Analysis of Gene Function

Frontiers in Cell and Developmental Biology, 2022
Mouse embryonic stem cells (ESCs) are powerful tools for functional analysis of stem cell-related genes; however, complex gene manipulations, such as locus-targeted introduction of multiple genes and conditional gene knockout conditional knockout, are ...
Teruhiko Suzuki, Satoko Takagi, Satoko Takagi, Takahiko Hara, Takahiko Hara, Takahiko Hara   +5 more
doaj   +1 more source

Use of integrase-minus lentiviral vector for transient expression [PDF]

, 2012
Objective: Lentivirus-derived vectors are among the most promising viral vectors for gene therapy which is currently available, but their use in clinical practice is limited due to associated risk of insertional mutagenesis.
Azadeh, H., Farazmandfar, T., Haghshenas, M.R., Janbabai, G., Samaei, N.M., Shahreza, H.K.   +5 more
core   +2 more sources

A Myelin Proteolipid Protein-LacZ Fusion Protein Is Developmentally Regulated and Targeted to the Myelin Membrane in Transgenic Mice [PDF]

, 1993
Transgenic mice were generated with a fusion gene carrying a portion of the murine myelin proteolipid protein (PLP) gene, including the first intron, fused to the E. coli LacZ gene.
Duchala, Cynthia S., Macklin, Wendy B., Readhead, Carol, Wight, Patricia A.   +3 more
core   +2 more sources

Adjunctive Therapeutic Plasma Exchange in Refractory Adult‐Onset Still's Disease Complicated by Secondary Macrophage Activation Syndrome: A Single‐Center Experience

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction Adult‐onset Still's disease (AOSD) complicated by macrophage activation syndrome (MAS) carries substantial mortality. The role of therapeutic plasma exchange (TPE) remains uncertain. Methods We retrospectively analyzed patients with AOSD‐MAS treated with TPE at a single‐center.
Masataka Ueda, Tomohiro Saito, Masahide Mizobuchi, Tomoki Shimomura, Saki Kawamori, Mitsuru Kawanishi, Natsuko Hattori, Jo Sugiyama, Satoshi Tsubata, Hanami Horiguchi, Kazuki Abe, Nao Iida, Yuki Kajio, Nao Oguro, Ryo Yanai, Hirokazu Honda   +15 more
wiley   +1 more source

In vitro correction of cystic fibrosis epithelial cell lines by small fragment homologous replacement (SFHR) technique

BMC Medical Genetics, 2002
Background SFHR (small fragment homologous replacement)-mediated targeting is a process that has been used to correct specific mutations in mammalian cells. This process involves both chemical and cellular factors that are not yet defined.
Bonifazi Emanuela, Nardone Anna, Serafino Annalucia, Bruscia Emanuela, Sangiuolo Federica, Lais Monica, Gruenert Dieter C, Novelli Giuseppe   +7 more
doaj   +1 more source

The promoter from SlREO, a highly-expressed, root-specific Solanum lycopersicum gene, directs expression to cortex of mature roots [PDF]

, 2008
Root-specific promoters are valuable tools for targeting transgene expression, but many of those already described have limitations to their general applicability.
Abe, Andrew J. Thompson, Bassett, Benfey, Bird, Bradford, Bucher, Bucher, Butler, Cardon, Carole Wright, Chan, Chen, Diatchenko, Elmayan, Fester, Garoosi, Giritch, Giritch, Gittins, Grichko, Gubler, Hagen, Hans, Hausinger, Hertig, Higo, Hudson, Ian B. Taylor, Itzhaki, Jefferson, John Andrews, Karimi, Keller, Kenneth Manning, Klinedinst, Koyama, Krasnyanski, K�ck, Liang, Liedl, L�on-Kloosterziel, Marin, Matsuda, Matthew O. Jones, Mudge, Mudge, Okubara, Phillips, Prescott, Prescott, Radi, Ro, Saslowsky, Sobeih, Subramanian, Suzuki, Tang, Thompson, Tirajoh, Vaughan, von Schweinichen, White, Xiao, Xu, Yamamoto, Yoshimoto, Zhang, Zhang   +68 more
core   +1 more source

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source