Results 61 to 70 of about 12,501,640 (402)

Improved Gene Targeting through Cell Cycle Synchronization

PLoS ONE, 2015
Gene targeting is a challenge in organisms where non-homologous end-joining is the predominant form of recombination. We show that cell division cycle synchronization can be applied to significantly increase the rate of homologous recombination during ...
Vasiliki Tsakraklides, E. Brevnova, G. Stephanopoulos, A. J. Shaw   +3 more
semanticscholar   +1 more source

FoxO1 signaling in B cell malignancies and its therapeutic targeting

FEBS Letters, EarlyView.
FoxO1 has context‐specific tumor suppressor or oncogenic character in myeloid and B cell malignancies. This includes tumor‐promoting properties such as stemness maintenance and DNA damage tolerance in acute leukemias, or regulation of cell proliferation and survival, or migration in mature B cell malignancies.
Krystof Hlavac, Petra Pavelkova, Laura Ondrisova, Marek Mraz   +3 more
wiley   +1 more source

Novel Heterotypic Rox Sites for Combinatorial Dre Recombination Strategies

G3: Genes, Genomes, Genetics, 2016
Site-specific recombinases (SSRs) such as Cre are widely used in gene targeting and genetic approaches for cell labeling and manipulation. They mediate DNA strand exchange between two DNA molecules at dedicated recognition sites. Precise understanding of
Katherine Chuang, Eileen Nguyen, Yuri Sergeev, Tudor C. Badea   +3 more
doaj   +1 more source

iPSC-Derived Intestinal Organoids from Cystic Fibrosis Patients Acquire CFTR Activity upon TALEN-Mediated Repair of the p.F508del Mutation

Molecular Therapy: Methods & Clinical Development, 2020
Cystic fibrosis (CF) is the main genetic cause of death among the Caucasian population. The disease is characterized by abnormal fluid and electrolyte mobility across secretory epithelia.
Aarne Fleischer, Sara Vallejo-Díez, José María Martín-Fernández, Almudena Sánchez-Gilabert, Mónica Castresana, Angel del Pozo, Amaia Esquisabel, Silvia Ávila, José Luis Castrillo, Eusebio Gaínza, José Luis Pedraz, Miguel Viñas, Daniel Bachiller   +12 more
doaj   +1 more source

Inhibition of Murine Cytomegalovirus Infection in Animals by RNase P-Associated External Guide Sequences. [PDF]

, 2017
External guide sequence (EGS) RNAs are associated with ribonuclease P (RNase P), a tRNA processing enzyme, and represent promising agents for gene-targeting applications as they can direct RNase-P-mediated cleavage of a target mRNA.
Li, Wei, LIU, Fenyong, Liu, Yujun, Lu, Sangwei, Sheng, Jingxue, Sun, Xu, Trang, Phong, Vu, Gia-Phong, Xu, Mengqiong, Yang, Zhu   +9 more
core   +2 more sources

Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia

FEBS Letters, EarlyView.
This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley   +1 more source

LPG2 Gene Duplication in Leishmania infantum: A Case for CRISPR-Cas9 Gene Editing

Frontiers in Cellular and Infection Microbiology, 2020
On the surface of the Leishmania promastigote, phosphoglycans (PG) such as lipophosphoglycan (LPG), proteophosphoglycan (PPG), free phosphoglycan polymers (PGs), and acid phosphatases (sAP), are dominant and contribute to the invasion and survival of ...
Flávio Henrique Jesus-Santos, Flávio Henrique Jesus-Santos, Jéssica Lobo-Silva, Pablo Ivan Pereira Ramos, Albert Descoteaux, Jonilson Berlink Lima, Valéria Matos Borges, Valéria Matos Borges, Leonardo Paiva Farias, Leonardo Paiva Farias   +9 more
doaj   +1 more source

Targeting the Apoa1 locus for liver-directed gene therapy

Molecular Therapy: Methods & Clinical Development, 2021
Clinical application of somatic genome editing requires therapeutics that are generalizable to a broad range of patients. Targeted insertion of promoterless transgenes can ensure that edits are permanent and broadly applicable while minimizing risks of ...
Marco De Giorgi, Ang Li, Ayrea Hurley, Mercedes Barzi, Alexandria M. Doerfler, Nikitha A. Cherayil, Harrison E. Smith, Jonathan D. Brown, Charles Y. Lin, Karl-Dimiter Bissig, Gang Bao, William R. Lagor   +11 more
doaj   +1 more source

The promoter from SlREO, a highly-expressed, root-specific Solanum lycopersicum gene, directs expression to cortex of mature roots [PDF]

, 2008
Root-specific promoters are valuable tools for targeting transgene expression, but many of those already described have limitations to their general applicability.
Abe, Andrew J. Thompson, Bassett, Benfey, Bird, Bradford, Bucher, Bucher, Butler, Cardon, Carole Wright, Chan, Chen, Diatchenko, Elmayan, Fester, Garoosi, Giritch, Giritch, Gittins, Grichko, Gubler, Hagen, Hans, Hausinger, Hertig, Higo, Hudson, Ian B. Taylor, Itzhaki, Jefferson, John Andrews, Karimi, Keller, Kenneth Manning, Klinedinst, Koyama, Krasnyanski, K�ck, Liang, Liedl, L�on-Kloosterziel, Marin, Matsuda, Matthew O. Jones, Mudge, Mudge, Okubara, Phillips, Prescott, Prescott, Radi, Ro, Saslowsky, Sobeih, Subramanian, Suzuki, Tang, Thompson, Tirajoh, Vaughan, von Schweinichen, White, Xiao, Xu, Yamamoto, Yoshimoto, Zhang, Zhang   +68 more
core   +1 more source

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

FEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro, Marco Cellani, Cristina Scielzo   +2 more
wiley   +1 more source