Results 81 to 90 of about 1,207,125 (312)
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Advances in gene targeting methods [PDF]
, 1997 Gene targeting in embryonic stem cells is commonly used for gene inactivation and the generation of mouse mutants. The combined use of methods for site-specific and homologous DNA recombination expands the potential of gene targeting in embryonic stem ...
Kühn, R. +3 more
core +1 more source
Novel Approach in the Construction of Bioethanol-Producing Saccharomyces cerevisiae Hybrids
Food Technology and Biotechnology, 2019 Bioethanol production from lignocellulosic hydrolysates requires a producer strain that tolerates both the presence of growth and fermentation inhibitors and high ethanol concentrations. Therefore, we constructed heterozygous intraspecies hybrid diploids
Anamarija Štafa +6 more
doaj +1 more source
Machine learning discriminates a movement disorder in a zebrafish model of Parkinson's disease
Disease Models & Mechanisms, 2020 Animal models of human disease provide an in vivo system that can reveal molecular mechanisms by which mutations cause pathology, and, moreover, have the potential to provide a valuable tool for drug development. Here, we have developed a zebrafish model
Gideon L. Hughes +5 more
doaj +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
FEBS Letters, EarlyView.Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil +4 more
wiley +1 more source
Biophysical approaches for studying viral entry
FEBS Letters, EarlyView.Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley +1 more source
Targeting Amyotrophic Lateral Sclerosis with Gene Therapy: From Silencing Genes to Enhancing Neuroprotection [PDF]
Elivery routes; Gene augmentation; Gene silencingVies d'administració; Augment gènic; Silenciament gènicVías de administración; Aumento génico; Silenciamiento génicoGene therapy is emerging as a transformative approach for treating amyotrophic lateral ...
Verdés-Franquesa, Sergi +2 more
core +1 more source
Nuclear Targeting Dynamics of Gold Nanoclusters for Enhanced Therapy of HER2(+) Breast Cancer [PDF]
, 2011 Recent advances in fluorescent metal nanoclusters have spurred tremendous interest In nanomedicine due to the ease of fabrication, excellent biocompatibility, and, more Importantly, excellent wavelength-dependent tunability.
Joseph Irudayaraj +5 more
core +2 more sources
Viruses, 2015 An engineered RNase P-based ribozyme variant, which was generated using the in vitro selection procedure, was used to target the overlapping mRNA region of two proteins essential for human cytomegalovirus (HCMV) replication: capsid assembly protein (AP ...
Zhu Yang +9 more
doaj +1 more source