Results 71 to 80 of about 10,802,441 (400)

Making tau amyloid models in vitro: a crucial and underestimated challenge

FEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source

Coordinated and widespread expression of γ-secretase in vivo: evidence for size and molecular heterogeneity

Neurobiology of Disease, 2004
γ-Secretase is a high molecular weight protein complex composed of four subunits, namely, presenilin (PS; 1 or 2), nicastrin, anterior pharynx defective-1 (Aph-1; A or B), and presenilin enhancer-2 (Pen-2), and is responsible for the cleavage of a number
Sébastien S. Hébert, Lutgarde Serneels, Tim Dejaegere, Katrien Horré, Michal Dabrowski, Veerle Baert, Wim Annaert, Dieter Hartmann, Bart De Strooper   +8 more
doaj  

Strong In Vivo Inhibition of HIV-1 Replication by Nullbasic, a Tat Mutant

mBio, 2019
Nullbasic is a mutant form of the HIV-1 transcriptional activator protein (Tat) that strongly inhibits HIV-1 transcription and replication in lymphocytes in vitro.
Hongping Jin, Yifan Sun, Dongsheng Li, Min-Hsuan Lin, Mary Lor, Lina Rustanti, David Harrich   +6 more
doaj   +1 more source

Horizontal gene transfer in chromalveolates [PDF]

BMC Evolutionary Biology, 2007
Horizontal gene transfer (HGT), the non-genealogical transfer of genetic material between different organisms, is considered a potentially important mechanism of genome evolution in eukaryotes. Using phylogenomic analyses of expressed sequence tag (EST) data generated from a clonal cell line of a free living dinoflagellate alga Karenia brevis, we ...
Tetyana Nosenko, Debashish Bhattacharya
openaire   +4 more sources

Carbohydrate receptor-mediated gene transfer to human T leukaemic cells [PDF]

, 1994
The mucin-type carbohydrate Tn cryptantigen (GalNAcα1-O-Ser/Thr, where GalNAc is N-acetyl-D-galactosamine) is expressed in many carcinomas, in haemopoietic disorders including the Tn syndrome, and on human immunodeficiency virus (HIV) coat glycoproteins,
Berger, Eric G., Birnstiel, Max L., Clausen, Henrik, Cotten, Matt, Dinter, André, Mechtler, Karl, Rusconi, Sandro, Thurnher, Martin, Wagner, Ernst   +8 more
core   +2 more sources

The cytoskeletal control of B cell receptor and integrin signaling in normal B cells and chronic lymphocytic leukemia

FEBS Letters, EarlyView.
In lymphoid organs, antigen recognition and B cell receptor signaling rely on integrins and the cytoskeleton. Integrins act as mechanoreceptors, couple B cell receptor activation to cytoskeletal remodeling, and support immune synapse formation as well as antigen extraction.
Abhishek Pethe, Tanja Nicole Hartmann
wiley   +1 more source

Social context prevents heat hormetic effects against mutagens during fish development

FEBS Letters, EarlyView.
This study shows that sublethal heat stress protects fish embryos against ultraviolet radiation, a concept known as ‘hormesis’. However, chemical stress transmission between fish embryos negates this protective effect. By providing evidence for the mechanistic molecular basis of heat stress hormesis and interindividual stress communication, this study ...
Lauric Feugere, Claudio Silva De Freitas, Adam Bates, Kenneth B. Storey, Pedro Beltran‐Alvarez, Katharina C. Wollenberg Valero   +5 more
wiley   +1 more source

A systematic screening assay identifies efficient small guide RNAs for CRISPR activation

Frontiers in Bioengineering and Biotechnology
CRISPR-mediated gene activation (CRISPRa) encompasses a growing field of biotechnological approaches with exciting implications for gene therapy. However, there is a lack of experimental validation tools for selecting efficient sgRNAs for downstream ...
Elin Arvidsson, Diana Duarte Lobo, Diana Duarte Lobo, Ermelinda Sabarese, Fabio Duarte, Rui Jorge Nobre, Rui Jorge Nobre, Rui Jorge Nobre, Luis Quintino, Cecilia Lundberg   +9 more
doaj   +1 more source

Migration and horizontal gene transfer divide microbial genomes into multiple niches

Nature Communications, 2015
Horizontal gene transfer is central to microbial evolution, because it enables genetic regions to spread horizontally through diverse communities. However, how gene transfer exerts such a strong effect is not understood.
R. Niehus, Sara Mitri, A. Fletcher, K. Foster   +3 more
semanticscholar   +1 more source

Gene transfer to the outflow tract [PDF]

Experimental Eye Research, 2016
Elevated intraocular pressure is the primary cause of open angle glaucoma. Outflow resistance exists within the trabecular meshwork but also at the level of Schlemm′s canal and further downstream within the outflow system. Viral vectors allow to take advantage of naturally evolved, highly efficient mechanisms of gene transfer, a process that is termed ...
Yalong Dang, Ralitsa Loewen, Hardik A. Parikh, Pritha Roy, Nils A. Loewen   +4 more
openaire   +4 more sources