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A fluorescence video-endoscopy technique for detection of gene transfer and expression
Gene Therapy, 1998The green fluorescent protein (GFP) has previously been adapted as a reported for gene transfer and expression in mammalian cells in culture and in tissue sections. Herein is described a new method for detecting GFP in situ within epithelia accessible to fiberoptic endoscopy by incorporating fluorescent filters for detection of GFP into an existing ...
T R, Flotte +5 more
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Gene Transfer Techniques in Whole Embryo Cultured Post-implantation Mouse Embryos
2013Gene transfer techniques such as electroporation and lipofection are powerful systems for investigating gene function. In this chapter we focus on the methods and applications of gene transfer into specific cells and tissues of post-implantation mouse embryos.
Daisuke, Sakai, Paul A, Trainor
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Gene transfer to plants: approaches and available techniques
1993Gene transfer to plants is hampered by a variety of biological problems: the plant cell wall is a perfect barrier and trap for DNA molecules; egg cells, sperm cells and zygotes, are virtually inaccessible; proembryos are extremely small and enclosed within solid tissues; the tiny cells of hidden meristems which contribute to the ‘germline’ may not be ...
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Gene Transfer Techniques to Study Neuropeptide Processing
Annual Review of Physiology, 1988G, Thomas, B A, Thorne, D E, Hruby
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Studies on the transfer techniques of three maize genes.
Chinese journal of biotechnology, 1997Maize transformation has been carried out through microprojectile bombardment, ultrasonication in a DNA buffer, and ovary-injection with a self-made microinjector. The plasmid pB48.415, which carries a 3'-truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation. Transgenic maize plants were obtained
G, Wang +4 more
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p53 Plasmid Preparation and Techniques for Analysis of Gene Transfer and Expression
2003Hepatocellular carcinoma (HCC) is one of the most common causes of cancer death worldwide and is especially prevalent in certain areas of Africa and Asia. The most important etiological factor is infection with the hepatitis B or C virus. Treatment is generally unsatisfactory as the majority of patients are not suitable for surgical resection and ...
R R, Mitry +5 more
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Horizontal gene transfer and adaptive evolution in bacteria
Nature Reviews Microbiology, 2021Brian J Arnold +2 more
exaly
Beyond horizontal gene transfer: the role of plasmids in bacterial evolution
Nature Reviews Microbiology, 2021Jerónimo Rodríguez-Beltrán +2 more
exaly
Gene transfer techniques for CNS organotypic cultures
Neuroscience Research, 2009Naofumi Uesaka +2 more
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