Results 261 to 270 of about 625,112 (296)

The technique of detecting the dispensed volume in gene transfer system

Fifth World Congress on Intelligent Control and Automation (IEEE Cat. No.04EX788), 2004
An in-line detecting system is presented in this paper, which utilizes scanning tunneling microscope (STM) technology to detect accurately the dispensed volume in gene transfer system, and then a close-loop controlling system for the dispensed volume can be constructed based on this detecting system.
null Zhang Ling, null Zhang Yum, null Yang Yimin, null Cen Hanbin, null Zhang Yingxia   +4 more
openaire   +1 more source

Gene transfer to plants: approaches and available techniques

1993
Gene transfer to plants is hampered by a variety of biological problems: the plant cell wall is a perfect barrier and trap for DNA molecules; egg cells, sperm cells and zygotes, are virtually inaccessible; proembryos are extremely small and enclosed within solid tissues; the tiny cells of hidden meristems which contribute to the ‘germline’ may not be ...
openaire   +1 more source

Growth Factors and Gene Transfer with DNA Strand Technique in Tendon Healing

Journal of Long-Term Effects of Medical Implants, 2002
Growth factors are likely to be critically involved in all aspects of tendon healing and many studies, in vitro and in vivo, have tried to define the role of growth factors in this process, and to determine appropriate strategies for their use in tissue engineering to improve the healing of tendons.
Feng, Zhang, William C, Lineaweaver
openaire   +2 more sources

Lipospermine-mediated gene transfer technique into murine cultured cortical cells

Journal of Neuroscience Methods, 1997
In order to transfer exogenous DNA into embryonic cortical cells, we have chosen a transfection technique using a synthetic lipospermine (dipalmitoylphosphatidylethanolamylspermine, DPPES) which complexes DNA molecules and allows their penetration into the intracellular compartment.
Sola, Brigitte, C, Staedel, Remy, Jean-Serge, A, Bahr, Behr, Jean-Paul   +4 more
openaire   +3 more sources

Studies on the transfer techniques of three maize genes.

Chinese journal of biotechnology, 1997
Maize transformation has been carried out through microprojectile bombardment, ultrasonication in a DNA buffer, and ovary-injection with a self-made microinjector. The plasmid pB48.415, which carries a 3'-truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation. Transgenic maize plants were obtained
G, Wang, H, Zhang, Q, Ding, Y, Xie, J, Dai   +4 more
openaire   +1 more source

Gene Transfer Techniques to Study Neuropeptide Processing

Annual Review of Physiology, 1988
G, Thomas, B A, Thorne, D E, Hruby
openaire   +3 more sources

Techniques of DNA-Mediated Gene Transfer for Eukaryotic Cells

1982
DNA transformation in bacteria has permitted detailed genetic analysis as well as the implementation of recombinant DNA technology. The utility of manipulation and cloning of genes in bacteria is well recognized. This chapter will concern the manipulation and cloning of genes in eukaryotic systems ranging from yeast to animal systems. Gene manipulation
Arthur P. Bollon, Saul J. Silverstein
openaire   +1 more source

Gene transfer techniques for the delivery of GDNF in Parkinson's disease.

Novartis Foundation symposium, 2000
Parkinson's disease (PD) is a neurodegenerative disorder characterized by motor disturbances caused by an alteration of the dopaminergic nigrostriatal system. Current symptomatic treatments for PD include dopaminergic drug administration, deep brain stimulation, ablative surgery and fetal cell transplantation.
Ridet JL, Déglon N, Aebischer P
openaire   +2 more sources

Techniques for Gene Transfer

1993
Barnabás Jenes, Helen Moore, Jun Cao, Wanggen Zhang, Ray Wu   +4 more
openaire   +1 more source

Horizontal gene transfer and adaptive evolution in bacteria

Nature Reviews Microbiology, 2021
Brian J Arnold, William P Hanage
exaly