Results 251 to 260 of about 621,194 (302)

Plasmid-mediated gene transfer in neurons using the biolistics technique

Journal of Neuroscience Methods, 1997
Biolistics has been developed as a system for gene delivery into plant cells, but has recently been introduced for transfection into mammalian tissue, including few attempts in neural cells. Basically, in this system the plasmid DNA of interest is coated onto small particles, that are accelerated by a particular driving force.
J E, Biewenga, O H, Destrée, L H, Schrama   +2 more
openaire   +2 more sources

Lipospermine-mediated gene transfer technique into murine cultured cortical cells

Journal of Neuroscience Methods, 1997
In order to transfer exogenous DNA into embryonic cortical cells, we have chosen a transfection technique using a synthetic lipospermine (dipalmitoylphosphatidylethanolamylspermine, DPPES) which complexes DNA molecules and allows their penetration into the intracellular compartment.
Sola, Brigitte, C, Staedel, Remy, Jean-Serge, A, Bahr, Behr, Jean-Paul   +4 more
openaire   +3 more sources

Techniques of Chromosome-Mediated Gene Transfer

1982
Functional genes can be transferred to eukaryotic cells by incubation with isolated metaphase chromosomes. Uptake occurs by phagocytosis and most of the ingested chromosomal DNA is degraded to small inactive fragments. The frequency of transfer of any selected marker ranges from about 10-5 to 10-7.
openaire   +1 more source

Gene Transfer Techniques and their relevance to Woody Plants

2000
For decades, the concept of foreign DNA transfer into plant cells and its integration into the genome of the recipient cell was an intriguing curiosity (Lurquin, 1977). It was not until the advent of Southern hybridization (Southern, 1975) and the availability of restriction enzymes that the presence of `non-plant’ DNA (in this case, the T-DNA of ...
Minocha, Subhash C., Wallace, John C.
openaire   +2 more sources

Development of a Gene Transfer Technique Using AFM

AIP Conference Proceedings, 2003
We report on a new low invasive technique for gene transfer of living cells using an ultra thin needle, termed a nano‐needle, comprising part of an etched atomic force microscope (AFM) tip. A silicon AFM tip was sharpened using focused ion beam etching to create an etched Si tip with a small diameter (100 to 200 nm).
openaire   +1 more source

Prospects and Possibilities for Gene Transfer Techniques in Insects

1993
The ability to analyze and manipulate organisms by classical genetic means has proven to be one of the most powerful tools for studying basic biological questions as well as for addressing important problems in agriculture and medicine. Today we have -available a new set of genetic tools, those of molecular genetics, which allow researchers to more ...
David A. O’Brochta, Alfred M. Handler
openaire   +1 more source

Methods and Role of Embryo Rescue Technique in Alien Gene Transfer

2013
Embryo abortion occurs frequently in wide crosses, and thus embryo rescue is required for survival of the next generation. Rescues are performed by either directly transferring the excised embryo to an artificial medium or indirectly through flower (ovary), immature seed (fertilized ovule), or pod (silique) culture.
Lulsdorf, Monika M., Ferrie, Alison, Slater, Susan M. H., Yuan, Hai Ying   +3 more
openaire   +2 more sources

Plant Transformation Techniques: Agrobacterium- and Microparticle-Mediated Gene Transfer in Cereal Plants

2020
Biotechnological methods for targeted gene transfers into plants are key for successful breeding in the twenty-first century and thus essential for the survival of humanity. Two decades ago, genetic transformation of crop plants was not routine, and it was all but impossible with important cereals such as barley and wheat.
Jafargholi, Imani, Karl-Heinz, Kogel
openaire   +2 more sources

Techniques of DNA-Mediated Gene Transfer for Eukaryotic Cells

1982
DNA transformation in bacteria has permitted detailed genetic analysis as well as the implementation of recombinant DNA technology. The utility of manipulation and cloning of genes in bacteria is well recognized. This chapter will concern the manipulation and cloning of genes in eukaryotic systems ranging from yeast to animal systems. Gene manipulation
Arthur P. Bollon, Saul J. Silverstein
openaire   +1 more source

Growth Factors and Gene Transfer with DNA Strand Technique in Tendon Healing

Journal of Long-Term Effects of Medical Implants, 2002
Growth factors are likely to be critically involved in all aspects of tendon healing and many studies, in vitro and in vivo, have tried to define the role of growth factors in this process, and to determine appropriate strategies for their use in tissue engineering to improve the healing of tendons.
Feng, Zhang, William C, Lineaweaver
openaire   +2 more sources