Results 301 to 310 of about 1,319,898 (363)

A fluorescence video-endoscopy technique for detection of gene transfer and expression [PDF]

Gene Therapy, 1998
The green fluorescent protein (GFP) has previously been adapted as a reported for gene transfer and expression in mammalian cells in culture and in tissue sections. Herein is described a new method for detecting GFP in situ within epithelia accessible to fiberoptic endoscopy by incorporating fluorescent filters for detection of GFP into an existing ...
Terence R. Flotte, Mark Potter, Sergei Zolotukhin, Kye Chesnut, S E Beck, Amy Poirier   +5 more
openaire   +2 more sources

The technique of detecting the dispensed volume in gene transfer system [PDF]

Fifth World Congress on Intelligent Control and Automation (IEEE Cat. No.04EX788), 2004
An in-line detecting system is presented in this paper, which utilizes scanning tunneling microscope (STM) technology to detect accurately the dispensed volume in gene transfer system, and then a close-loop controlling system for the dispensed volume can be constructed based on this detecting system.
Zhang Ling, Zhang Ying-xia, Yang Yi-min, Cen Hanbin, Zhang Yun   +4 more
openaire   +1 more source

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New therapeutic approaches based on gene transfer techniques

Springer Seminars in Immunopathology, 1996
The experimental results from the use of gene transfer techniques for cancer therapy are promising but, at the same time, somewhat perplexing. Progress will depend on the further understanding of the biology of tumour interaction with the immune system, as this would be important for the development of optimal strategies for harnessing an effective ...
Heung Chong, Richard G. Vile
openaire   +3 more sources

In vivo nasal potential difference: techniques and protocols for assessing efficacy of gene transfer in cystic fibrosis.

Human Gene Therapy, 1995
Cystic fibrosis (CF) is a monogenetic disease that is associated with chronic airways disease and early death. The pulmonary disease reflects mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, and associated abnormal ...
M. Knowles, A. M. Paradiso, R. Boucher
semanticscholar   +1 more source

Development of a Gene Transfer Technique Using AFM

AIP Conference Proceedings, 2003
We report on a new low invasive technique for gene transfer of living cells using an ultra thin needle, termed a nano‐needle, comprising part of an etched atomic force microscope (AFM) tip. A silicon AFM tip was sharpened using focused ion beam etching to create an etched Si tip with a small diameter (100 to 200 nm).
N. Nakamura, Hiroshi Tokumoto, Sung-Woong Han, Jun Miyake, I. Obataya, Masami Kageshima, Seiji Takeda, C. Nakamura   +7 more
openaire   +2 more sources

Gene transfer techniques for the delivery of GDNF in Parkinson's disease.

Novartis Foundation symposium, 2000
Parkinson's disease (PD) is a neurodegenerative disorder characterized by motor disturbances caused by an alteration of the dopaminergic nigrostriatal system. Current symptomatic treatments for PD include dopaminergic drug administration, deep brain stimulation, ablative surgery and fetal cell transplantation.
Jean-Luc Ridet, Nicole Déglon, Patrick Aebischer   +2 more
openaire   +3 more sources

Methods and Role of Embryo Rescue Technique in Alien Gene Transfer [PDF]

, 2013
Embryo abortion occurs frequently in wide crosses, and thus embryo rescue is required for survival of the next generation. Rescues are performed by either directly transferring the excised embryo to an artificial medium or indirectly through flower (ovary), immature seed (fertilized ovule), or pod (silique) culture.
Lulsdorf, Monika M., Ferrie, Alison, Slater, Susan M. H., Yuan, Hai Ying   +3 more
openaire   +2 more sources

Gene Transfer with DNA Strand Technique and Peripheral Nerve Injuries

Journal of Long-Term Effects of Medical Implants, 2002
Although much progress has been made, current treatments of peripheral nerve injury often result in only partial recovery. Many methods have been used to improve the surgical exploration for injured nerve repair. However, much less is known about the involvement of complex neurochemical interactions. Several neurotrophic factors have been shown to play
Feng Zhang, William C. Lineaweaver
openaire   +3 more sources

Prospects and Possibilities for Gene Transfer Techniques in Insects

1993
The ability to analyze and manipulate organisms by classical genetic means has proven to be one of the most powerful tools for studying basic biological questions as well as for addressing important problems in agriculture and medicine. Today we have -available a new set of genetic tools, those of molecular genetics, which allow researchers to more ...
Alfred M. Handler, David A. O'Brochta
openaire   +2 more sources

Plasmid-mediated gene transfer in neurons using the biolistics technique

Journal of Neuroscience Methods, 1997
Biolistics has been developed as a system for gene delivery into plant cells, but has recently been introduced for transfection into mammalian tissue, including few attempts in neural cells. Basically, in this system the plasmid DNA of interest is coated onto small particles, that are accelerated by a particular driving force.
Loes H. Schrama, Jeike E. Biewenga, Olivier H.J. Destrée   +2 more
openaire   +3 more sources