Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells
Journal of Biomedical Science, 2010 Background Gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. However, effective transfection of postmitotic neurons remains a challenge.
Wu Chih-Hsien +7 more
doaj +1 more source
Generation of mutant pigs by lipofection-mediated genome editing in embryos
Scientific Reports, 2021 The specificity and efficiency of CRISPR/Cas9 gene-editing systems are determined by several factors, including the mode of delivery, when applied to mammalian embryos.
Maki Hirata +10 more
doaj +1 more source
Going nuclear: gene family evolution and vertebrate phylogeny reconciled [PDF]
, 2002 Gene duplications have been common throughout vertebrate evolution, introducing paralogy and so complicating phylogenctic inference from nuclear genes.
James A. Cotton +6 more
core +4 more sources
The role of fibroblast growth factors in cell and cancer metabolism
FEBS Letters, EarlyView.Fibroblast growth factor (FGF) signaling regulates crucial signaling cascades that promote cell proliferation, survival, and metabolism. Therefore, FGFs and their receptors are often dysregulated in human diseases, including cancer, to sustain proliferation and rewire metabolism.
Jessica Price, Chiara Francavilla
wiley +1 more source
PLoS ONE, 2017 Reduced pesticide use is one of the reasons given by Europeans for accepting new genetic engineering techniques. According to the advocates of these techniques, consumers are likely to embrace the application of cisgenesis to apple trees.
Damien Rousselière, Samira Rousselière
doaj +1 more source
Proteomics as the final step in the functional metagenomics study of antimicrobial resistance [PDF]
, 2015 peer-reviewedThe majority of clinically applied antimicrobial agents are derived from natural products generated by soil microorganisms and therefore resistance is likely to be ubiquitous in such environments.
Cotter, Paul D. +4 more
core +1 more source
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
FEBS Letters, EarlyView.This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
Evolutionary optimization within an intelligent hybrid system for design integration [PDF]
, 1999 An intelligent hybrid approach has been developed to integrate various stages in total design, including formulation of product design specifications, conceptual design, detail design, and manufacture.
Su, D, Wakelam, M
core +1 more source
The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells
FEBS Letters, EarlyView.The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman +3 more
wiley +1 more source
Targeting gene expression to specific cells of kidney tubules in vivo, using adenoviral promoter fragments. [PDF]
PLoS ONE, 2017 Although techniques for cell-specific gene expression via viral transfer have advanced, many challenges (e.g., viral vector design, transduction of genes into specific target cells) still remain.
Sumiyo Watanabe +10 more
doaj +1 more source