Results 41 to 50 of about 621,194 (302)

Unintended Genomic Outcomes in Current and Next Generation GM Techniques: A Systematic Review

Plants, 2022
Classical genetic engineering and new genome editing techniques, especially the CRISPR/Cas technology, increase the possibilities for modifying the genetic material in organisms. These technologies have the potential to provide novel agricultural traits,
Philomena Chu, Sarah Zanon Agapito-Tenfen   +1 more
doaj   +1 more source

Asymmetric somatic plant hybridization : status and applications [PDF]

, 2013
To create asymmetric somatic hybrids, the genome of the so-called donor protoplast is fragmented prior to protoplast fusion. As a result, only a limited amount of the donor genome is transferred to the fusion product.
Deryckere, Dieter, Eeckhaut, Tom, Lakshmanan, Prabhu Shankar, Van Bockstaele, Erik, Van Huylenbroeck, Johan   +4 more
core   +2 more sources

A flow cytometry technique for measuring chromosome-mediated gene transfer [PDF]

Cytometry, 2001
Using artificial chromosome expression systems (ACes), we have developed a unique and rapid screening technique to quantify delivery of foreign DNA into cells in vitro. Delivery was measured within 24 h after transfection, using flow cytometry to detect the transfer of ACes labeled with thymidine analogue.
S, Vanderbyl, N, MacDonald, G, de Jong
openaire   +2 more sources

Optimization of human papillomavirus-based pseudovirus techniques for efficient gene transfer [PDF]

Scientific Reports, 2020
Abstract Human papillomavirus (HPV) L1 and L2 capsid proteins self-assemble into virions capable of efficiently packaging either its 8 kilobase genome or non-viral DNA. The ability of HPV capsids to package non-viral DNA makes these a useful tool for delivering plasmids to study proteins of interest in a variety of cell types.
Gilson, Timra D., Gibson, Ryan T., Androphy, Elliot J.   +2 more
openaire   +2 more sources

Functional knowledge transfer for high-accuracy prediction of under-studied biological processes. [PDF]

PLoS Computational Biology, 2013
A key challenge in genetics is identifying the functional roles of genes in pathways. Numerous functional genomics techniques (e.g. machine learning) that predict protein function have been developed to address this question.
Christopher Y Park, Aaron K Wong, Casey S Greene, Jessica Rowland, Yuanfang Guan, Lars A Bongo, Rebecca D Burdine, Olga G Troyanskaya   +7 more
doaj   +1 more source

Transmission of mitochondrial DNA following assisted reproduction and nuclear transfer [PDF]

, 2006
Review of the articleMitochondria are the organelles responsible for producing the majority of a cell's ATP and also play an essential role in gamete maturation and embryo development.
Alam, Almeida-Santos, Anderson, Ankel-Simons, Barritt, Bavister, Blok, Boulet, Brenner, Brison, Brown, Brown, Campbell, Chang, Chen, Chen, Chinnery, Chung, Cibelli, Cohen, Cortesini, Cui, Cummins, Cummins, Dailey, Danan, Davis, De Block, Dean, Dumollard, Dunbar, D’Aurelio, E.C. Spikings, Eichenlaub-Ritter, Eisen, El Shourbagy, Falkenberg, Fisher, Fisher, Fulka, Gahan, Giles, Graziewicz, Gyllensten, Hammer, Han, Hance, Hanna, Hauswirth, He, Hecht, Hiendleder, Hiendleder, Hiendleder, Holden, Houghton, Humpherys, Inoue, Irwin, J. Alderson, J.C.St. John, Jansen, Kaneda, Keefe, Kenyon, King, Kirches, Klopstock, Koehler, Kondo, Kondo, Kong, Kong, Kraytsberg, Kvist, Laflamme, Larsson, Larsson, Larsson, Larsson, Leese, Lertrit, Lestienne, Levy, Lin, Liu, Liu, Liu, Lloyd, Ludwig, Luetjens, Machaty, Magnusson, Mancuso, Marchington, Margineantu, May-Panloup, McConnell, McCreath, McKenzie, McKenzie, McKenzie, Meirelles, Meirelles, Meirelles, Meusel, Michaels, Monnat, Moyes, Munne, Munsie, Nagao, Naini, Nakamura, Naviaux, Nishi, Nishigaki, Nishimura, Normile, Ojala, Ono, Palermo, Pfeiffer, Piko, Piko, Ponzetto, Poulton, Reynier, Reynier, Rideout, Rubart, Ruiz-Pesini, Sathananthan, Sathananthan, Sato, Satoh, Sazer, Scarpulla, Schon, Schwartz, Seifer, Sherratt, Shitara, Shoubridge, Siciliano, Smith, Sokolova, Spelbrink, Spiropoulos, St. John, St. John, St. John, St. John, St. John, St. John, St. John, St. John, Steinborn, Steinborn, Steuerwald, Stojanovski, Stojkovic, Sturmey, Sun, Sun, Suomalainen, Sutovsky, Sutovsky, Takeda, Takeda, Takeda, Takeuchi, Takeuchi, Taylor, Tessa, Thompson, Tiranti, Trounce, Trounson, Tsaousis, Vajta, Van Blerkom, Van Blerkom, Van Blerkom, Van Goethem, Wakayama, Wallace, Wallace, Wernig, White, White, Wiesner, Wilding, Wilmut, Xu, Yakes, Yang, Yang, Zhang, Zhao, Zouros   +200 more
core   +2 more sources

Impact of baculoviral transduction of fluorescent actin on cellular forces

European Journal of Cell Biology, 2023
Live staining of actin brings valuable information in the field of mechanobiology. Gene transfer of GFP-actin has been reported to disturb cell rheological properties while gene transfer of fluorescent actin binding proteins was not.
Sara Bouizakarne, Jocelyn Etienne, Alice Nicolas   +2 more
doaj   +1 more source

Gene Therapy in the Management of Erectile Dysfunction (ED): Past, Present, and Future

The Scientific World Journal, 2009
In the past, many researchers considered viral vectors to be the most promising candidates to transfer genetic material into the corpora for the treatment of erectile dysfunction. However, at present, no viral vectors have progressed to human trials.
Arnold Melman, Kelvin P. Davies
doaj   +1 more source

Small extracellular vesicle DNA-mediated horizontal gene transfer as a driving force for tumor evolution: Facts and riddles

Frontiers in Oncology, 2022
The basis of the conventional gene-centric view on tumor evolution is that vertically inherited mutations largely define the properties of tumor cells.
Gábor Valcz, Beáta Újvári, Edit I. Buzás, Edit I. Buzás, Edit I. Buzás, Tibor Krenács, Sándor Spisák, Ágnes Kittel, Zsolt Tulassay, Péter Igaz, Péter Igaz, Péter Igaz, István Takács, Béla Molnár, Béla Molnár   +14 more
doaj   +1 more source

Stable Feature Selection for Biomarker Discovery [PDF]

, 2010
Feature selection techniques have been used as the workhorse in biomarker discovery applications for a long time. Surprisingly, the stability of feature selection with respect to sampling variations has long been under-considered.
He, Zengyou, Yu, Weichuan
core   +2 more sources