Results 21 to 30 of about 246,465 (258)
Unintended Genomic Outcomes in Current and Next Generation GM Techniques: A Systematic Review
Plants, 2022 Classical genetic engineering and new genome editing techniques, especially the CRISPR/Cas technology, increase the possibilities for modifying the genetic material in organisms. These technologies have the potential to provide novel agricultural traits,
Philomena Chu +1 more
doaj +1 more source
Irreversible electroporation is a surgical ablation technique that enhances gene transfer [PDF]
Surgery, 2011 Reversible electroporation has long been used to transfer macromolecules into target cells in the laboratory by using an electric field to induce transient membrane permeability. Recently, the electric field has been modulated to produce permanent membrane permeability and cell death.
Joyce T, Au +10 more
openaire +2 more sources
Liposomes as a gene delivery system
Brazilian Journal of Medical and Biological Research, 1999 Gene therapy is an active field that has progressed rapidly into clinical trials in a relatively short time. The key to success for any gene therapy strategy is to design a vector able to serve as a safe and efficient gene delivery vehicle.
C. Ropert
doaj +1 more source
Functional knowledge transfer for high-accuracy prediction of under-studied biological processes. [PDF]
PLoS Computational Biology, 2013 A key challenge in genetics is identifying the functional roles of genes in pathways. Numerous functional genomics techniques (e.g. machine learning) that predict protein function have been developed to address this question.
Christopher Y Park +7 more
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Impact of baculoviral transduction of fluorescent actin on cellular forces
European Journal of Cell Biology, 2023 Live staining of actin brings valuable information in the field of mechanobiology. Gene transfer of GFP-actin has been reported to disturb cell rheological properties while gene transfer of fluorescent actin binding proteins was not.
Sara Bouizakarne +2 more
doaj +1 more source
A flow cytometry technique for measuring chromosome-mediated gene transfer [PDF]
Cytometry, 2001 Using artificial chromosome expression systems (ACes), we have developed a unique and rapid screening technique to quantify delivery of foreign DNA into cells in vitro. Delivery was measured within 24 h after transfection, using flow cytometry to detect the transfer of ACes labeled with thymidine analogue.
S, Vanderbyl, N, MacDonald, G, de Jong
openaire +2 more sources
Gene Therapy in the Management of Erectile Dysfunction (ED): Past, Present, and Future
The Scientific World Journal, 2009 In the past, many researchers considered viral vectors to be the most promising candidates to transfer genetic material into the corpora for the treatment of erectile dysfunction. However, at present, no viral vectors have progressed to human trials.
Arnold Melman, Kelvin P. Davies
doaj +1 more source
Frontiers in Oncology, 2022 The basis of the conventional gene-centric view on tumor evolution is that vertically inherited mutations largely define the properties of tumor cells.
Gábor Valcz +14 more
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PLoS ONE, 2012 The domestic chicken is an attractive model system to explore the development and function of brain circuits. Electroporation-mediated and retrovirus (including lentivirus) vector-mediated gene transfer techniques have been widely used to introduce ...
Ryosuke Matsui +2 more
doaj +1 more source
Personalized Zebrafish Models for Fusion‐Positive Pediatric Sarcomas
Pediatric Blood &Cancer, EarlyView.ABSTRACT Clinical sequencing efforts have revolutionized our approaches to categorizing pediatric cancers in real time. This has dramatically improved our ability to profile pediatric tumors, identify actionable vulnerabilities, and influence clinical care.
Lisa H. Hall +2 more
wiley +1 more source