Results 11 to 20 of about 246,465 (258)

The challenging nature of primary T lymphocytes for transfection: Effect of protamine sulfate on the transfection efficiency of chemical transfection reagents

Research in Pharmaceutical Sciences, 2020
Background and purpose: The optimization of an effective non-viral gene delivery method for genetic manipulation of primary human T cells has been a major challenge in immunotherapy researches.
Ilnaz Rahimmanesh, Mehdi Totonchi, Hossein Khanahmad   +2 more
doaj   +1 more source

Calcium Based Non-viral Gene Delivery: An Overview of Methodology and Applications [PDF]

Acta Medica Iranica, 2010
Application of therapeutic gene transfer in the treatment of genetic diseases is a notable progress but there are some disadvantages and limitations in it. The process of overcoming these barriers is a drastic change in gene delivery.
Kianoush Khosravi-Darani, Mohamaad Reza Mozafari, Ladan Rashidi, Mehrdad Mohammadi   +3 more
doaj   +1 more source

Construcción de un vector de expresión derivado de virus adenoasociados para corregir in vitro el defecto genético de la enfermedad de Morquio A

Biomédica: revista del Instituto Nacional de Salud, 2008
Introducción. La mucopolisacaridosis IV A (Morquio A) es una enfermedad de depósito lisosómico causada por la deficiencia en la actividad de la enzima N-acetil-galactosamina- 6-sulfato-sulfatasa que produce la acumulación intralisosómica de queratán y ...
Luis Alejandro Barrera, Mónica A. Gutiérrez, Felipe García Vallejo, Shunji Tomatsu, Flavio Cerón, Carlos J. Alméciga Díaz, Martha C. Domínguez   +6 more
doaj   +1 more source

Immunotherapy with adoptive cytomegalovirus‐specific T cells transfer: Summarizing latest gene engineering techniques

Health Science Reports, 2021
Cytomegalovirus (CMV) infection remains a major complication following allogeneic hematopoietic stem cell transplantation (HSCT). T cell response plays a critical role in inducing long‐term immunity against CMV infection/reactivation that impairs during ...
Mahshid Mehdizadeh, Samira Karami, Haniyeh Ghaffari Nazari, Ghazaleh Sankanian, Mohsen Hamidpour, Abbas Hajifathali   +5 more
doaj   +1 more source

Synthesis, characterization and evaluation of transfection efficiency of dexamethasone conjugated poly(propyleneimine) nanocarriers for gene delivery#

Pharmaceutical Biology, 2018
Context: Polypropylenimine (PPI), a cationic dendrimer with defined structure and positive surface charge, is a potent non-viral vector. Dexamethasone (Dexa) conveys to the nucleus through interaction with its intracellular receptor.
Bizhan Malaekeh-Nikouei, Mehdi Rezaee, Leila Gholami, Naghmeh Sanjar Mousavi, Reza Kazemi Oskuee   +4 more
doaj   +1 more source

Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques

BMC Biotechnology, 2011
Background Gene therapy continues to hold great potential for treating many different types of disease and dysfunction. Safe and efficient techniques for gene transfer and expression in vivo are needed to enable gene therapeutic strategies to be ...
Daniell Henry, Martinez Angela, Xia Jixiang, Ebert Steven N   +3 more
doaj   +1 more source

COMPARISON OF THREE DIFFERENT TECHNIQUES OF GENE TRANSFER IN HUMPBACK GROUPER ( ) CROMILEPTES ALTIVELIS

Biotropia: The Southeast Asian Journal of Tropical Biology, 2011
Humpback grouper is one of the most cultured fishes in Asia, including Indonesia. The main problemfaced by humpback culture is its slow growth rate.One of themethods that will be more effective and efficient to solve the problem is using transgenic ...
KOMAR SUMANTADINATA, SLAMET SUBYAKTO, ALIMUDDIN, RUSTIDJA, M. SASMITO JATI, IRVAN FAIZAL, RATU SITI ALIAH, GEMI TRIASTUTIK   +7 more
doaj   +3 more sources

Genetic Modification of T Cells

Biomedicines, 2016
Gene transfer technology and its application to human gene therapy greatly expanded in the last decade. One area of investigation that appears particularly promising is the transfer of new genetic material into T cells for the potential treatment of ...
Richard A. Morgan, Benjamin Boyerinas
doaj   +1 more source

A Microfluidic System of Gene Transfer by Ultrasound

Micromachines, 2022
Ultrasonic gene transfer has advantages beyond other cell transfer techniques because ultrasound does not directly act on cells, but rather pushes the gene fragments around the cells into cells through an acoustic hole effect. Most examples reported were
Cuimin Sun, Menghua Zhang, Guangyong Huang, Ping Zhang, Ronghui Lin, Xiangjun Wang, Hui You   +6 more
doaj   +1 more source

Highly efficient retrograde gene transfer into motor neurons by a lentiviral vector pseudotyped with fusion glycoprotein. [PDF]

PLoS ONE, 2013
The development of gene therapy techniques to introduce transgenes that promote neuronal survival and protection provides effective therapeutic approaches for neurological and neurodegenerative diseases.
Miyabi Hirano, Shigeki Kato, Kenta Kobayashi, Tomoaki Okada, Hiroyuki Yaginuma, Kazuto Kobayashi   +5 more
doaj   +1 more source