Results 11 to 20 of about 625,112 (296)

Cotton Biotechnology: An Efficient Gene Transfer Protocol via Agrobacterium tumefaciens for a Greater Transgenic Recovery

Journal of Natural Fibers, 2022
Due to its economic worth, cotton (Gossypium hirsutum L.) is grown in almost 70 countries and provides income for more than 250 million people. Therefore, producing cotton with having some desired characteristics that includes extended biotic and abiotic
Ibrahim Ilker Ozyigit, Ilhan Dogan, Yilmaz Kaya, Kasim Bajrovic, Nermin Gozukirmizi   +4 more
doaj   +1 more source

The challenging nature of primary T lymphocytes for transfection: Effect of protamine sulfate on the transfection efficiency of chemical transfection reagents

Research in Pharmaceutical Sciences, 2020
Background and purpose: The optimization of an effective non-viral gene delivery method for genetic manipulation of primary human T cells has been a major challenge in immunotherapy researches.
Ilnaz Rahimmanesh, Mehdi Totonchi, Hossein Khanahmad   +2 more
doaj   +1 more source

1-aminocyclopropane-1-carboxylate deaminase-producing Agrobacterium tumefaciens has higher ability for gene transfer into plant cells [PDF]

, 2008
Agrobacterium-mediated gene transfer is widely used for plant molecular genetics, and efficient techniques are required. Recent studies show that ethylene inhibits the gene transfer.
Ezura Hiroshi, Minamisawa Kiwamu, Nonaka Satoko, Sugawara Masayuki, Yuhashi Ken-ichi, 江面 浩   +5 more
core   +1 more source

Calcium Based Non-viral Gene Delivery: An Overview of Methodology and Applications [PDF]

Acta Medica Iranica, 2010
Application of therapeutic gene transfer in the treatment of genetic diseases is a notable progress but there are some disadvantages and limitations in it. The process of overcoming these barriers is a drastic change in gene delivery.
Kianoush Khosravi-Darani, Mohamaad Reza Mozafari, Ladan Rashidi, Mehrdad Mohammadi   +3 more
doaj   +1 more source

Carbohydrate receptor-mediated gene transfer to human T leukaemic cells [PDF]

, 1994
The mucin-type carbohydrate Tn cryptantigen (GalNAcα1-O-Ser/Thr, where GalNAc is N-acetyl-D-galactosamine) is expressed in many carcinomas, in haemopoietic disorders including the Tn syndrome, and on human immunodeficiency virus (HIV) coat glycoproteins,
Berger, Eric G., Birnstiel, Max L., Clausen, Henrik, Cotten, Matt, Dinter, André, Mechtler, Karl, Rusconi, Sandro, Thurnher, Martin, Wagner, Ernst   +8 more
core   +2 more sources

Construcción de un vector de expresión derivado de virus adenoasociados para corregir in vitro el defecto genético de la enfermedad de Morquio A

Biomédica: revista del Instituto Nacional de Salud, 2008
Introducción. La mucopolisacaridosis IV A (Morquio A) es una enfermedad de depósito lisosómico causada por la deficiencia en la actividad de la enzima N-acetil-galactosamina- 6-sulfato-sulfatasa que produce la acumulación intralisosómica de queratán y ...
Luis Alejandro Barrera, Mónica A. Gutiérrez, Felipe García Vallejo, Shunji Tomatsu, Flavio Cerón, Carlos J. Alméciga Díaz, Martha C. Domínguez   +6 more
doaj   +1 more source

Immunotherapy with adoptive cytomegalovirus‐specific T cells transfer: Summarizing latest gene engineering techniques

Health Science Reports, 2021
Cytomegalovirus (CMV) infection remains a major complication following allogeneic hematopoietic stem cell transplantation (HSCT). T cell response plays a critical role in inducing long‐term immunity against CMV infection/reactivation that impairs during ...
Mahshid Mehdizadeh, Samira Karami, Haniyeh Ghaffari Nazari, Ghazaleh Sankanian, Mohsen Hamidpour, Abbas Hajifathali   +5 more
doaj   +1 more source

Chimeric piggyBac transposases for genomic targeting in human cells. [PDF]

, 2012
Integrating vectors such as viruses and transposons insert transgenes semi-randomly and can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location of insertion is required ...
Belcaid, Mahdi, Coates, Craig J, Dang, Nong C, Maragathavally, Kommineni J, Moisyadi, Stefan, Owens, Jesse B, Segal, David J, Stoytchev, Ilko, Stoytcheva, Zoia, Urschitz, Johann   +9 more
core   +5 more sources

Synthesis, characterization and evaluation of transfection efficiency of dexamethasone conjugated poly(propyleneimine) nanocarriers for gene delivery#

Pharmaceutical Biology, 2018
Context: Polypropylenimine (PPI), a cationic dendrimer with defined structure and positive surface charge, is a potent non-viral vector. Dexamethasone (Dexa) conveys to the nucleus through interaction with its intracellular receptor.
Bizhan Malaekeh-Nikouei, Mehdi Rezaee, Leila Gholami, Naghmeh Sanjar Mousavi, Reza Kazemi Oskuee   +4 more
doaj   +1 more source

Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques

BMC Biotechnology, 2011
Background Gene therapy continues to hold great potential for treating many different types of disease and dysfunction. Safe and efficient techniques for gene transfer and expression in vivo are needed to enable gene therapeutic strategies to be ...
Daniell Henry, Martinez Angela, Xia Jixiang, Ebert Steven N   +3 more
doaj   +1 more source