Results 191 to 200 of about 361,847 (262)

Enhancing cold tolerance through HubZIP6-mediated CBF activation and salicylic acid signaling in pitaya. [PDF]

Mol Hortic
Hu X, Sabir IA, Xu Z, Zhang Z, Zhao J, Hu G, Qin Y.   +6 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Unbiased screen of human transcriptome reveals an unexpected role of 3'UTRs in translation initiation. [PDF]

Nat Commun
Yang Y, Fan X, Ye Y, Zhang Z, Chen C, Ludwig SEJ, Zhang S, Lu Q, Will CL, Urlaub H, Sun J, Lührmann R, Wang Z.   +12 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Perturbing H-NS function reveals roles in restricting virulence heterogeneity and pathogen adaptation. [PDF]

PLoS Pathog
McLelland L, Spratt M, Karki D, Marand A, Burt M, Zhao J, Lane K.   +6 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Transcriptional regulation of monolignol O-methylation in Cleome hassleriana. [PDF]

Plant J
Zhuo C, Burks D, Xiao X, Azad RK, Rao X, Chen F, Dixon RA.   +6 more
europepmc   +1 more source

Single-nucleus transcriptomics resolves multiple fate dynamics between inflorescence meristem and primary stem. [PDF]

Sci Adv
Moreno-Ramírez S, Lenz MO, Meyerowitz EM, Locke JCW, Jönsson H.   +4 more
europepmc   +1 more source

The Drosophila CEBPG homolog Irbp18 partners with crc (ATF4) to mediate the integrated stress response in degenerative disease models. [PDF]

Cell Rep
Mitra S, Huang HW, Faruk R, Low A, Yeo AI, Ryoo HD.   +5 more
europepmc   +1 more source