Results 21 to 30 of about 5,250,240 (290)

Molecular Imaging with Reporter Genes: Has Its Promise Been Delivered?

Journal of Nuclear Medicine, 2019
The first reporter systems were developed in the early 1980s and were based on measuring the activity of an enzyme—as a surrogate measure of promoter-driven transcriptional activity—which is now known as a reporter gene system.
I. Serganova, R. Blasberg
semanticscholar   +1 more source

A BAC-bacterial recombination method to generate physically linked multiple gene reporter DNA constructs

BMC Biotechnology, 2009
Background Reporter gene mice are valuable animal models for biological research providing a gene expression readout that can contribute to cellular characterization within the context of a developmental process.
Gong Shiaochin, Rowe David W, Liu Yaling, Stover Mary, Maye Peter, Lichtler Alexander C   +5 more
doaj   +1 more source

Overview of the reporter genes and reporter mouse models

Animal Models and Experimental Medicine, 2018
Reporter genes are widely applied in biotechnology and biomedical research owning to their easy observation and lack of toxicity. Taking advantage of the reporter genes in conjunction with imaging technologies, a large number of reporter mouse models ...
Shun Li, Lixiang Chen, Xiu-Hua Peng, Chao Wang, Bo‐yin Qin, Dan Tan, Cheng‐xiao Han, Hua Yang, X. Ren, Fang Liu, Chun-Hua Xu, Xiao-Hui Zhou   +11 more
semanticscholar   +1 more source

Use of Reporter Genes in the Generation of Vaccinia Virus-Derived Vectors

Viruses, 2016
Vaccinia virus (VACV) is one of the most extensively-studied viruses of the Poxviridae family. It is easy to genetically modify, so it has become a key tool for many applications.
Sally Al Ali, Sara Baldanta, Mercedes Fernández-Escobar, Susana Guerra   +3 more
doaj   +1 more source

Ectopic T cell receptor-α locus control region activity in B cells is suppressed by direct linkage to two flanking genes at once. [PDF]

PLoS ONE, 2010
The molecular mechanisms regulating the activity of the TCRα gene are required for the production of the circulating T cell repertoire. Elements of the mouse TCRα locus control region (LCR) play a role in these processes. We previously reported that TCRα
Stefan Knirr, Janette Gomos-Klein, Blanca E Andino, Faith Harrow, Karl F Erhard, Damian Kovalovsky, Derek B Sant'Angelo, Benjamin D Ortiz   +7 more
doaj   +1 more source

Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering

Scientific Reports, 2014
The type II bacterial CRISPR/Cas9 system is rapidly becoming popular for genome-engineering due to its simplicity, flexibility and high efficiency. Recently, targeted knock-in of a long DNA fragment via homology-independent DNA repair has been achieved ...
Yukiko Kimura, Yu Hisano, A. Kawahara, S. Higashijima   +3 more
semanticscholar   +1 more source

Reporter gene comparison demonstrates interference of complex body fluids with secreted luciferase activity

Scientific Reports, 2021
Reporter gene assays are widely used to study cellular signaling and transcriptional activity. Few studies describe the use of reporter genes for studying cellular responses on complex body fluids, such as urine and blood.
M. Neefjes, B. A. C. Housmans, G. G. H. van den Akker, L. W. van Rhijn, T. J. M. Welting, P. M. van der Kraan   +5 more
doaj   +1 more source

Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage

Development, 2014
We have generated the first transgenic chickens in which reporter genes are expressed in a specific immune cell lineage, based upon control elements of the colony stimulating factor 1 receptor (CSF1R) locus.
A. Balic, C. García-Morales, L. Vervelde, H. Gilhooley, A. Sherman, V. Garceau, M. Gutowska, D. Burt, P. Kaiser, D. Hume, H. Sang   +10 more
semanticscholar   +1 more source

Adenovirus-mediated gene transfer in primary murine adipocytes

Journal of Lipid Research, 2000
The transfer of genes into primary murine adipocytes using an adenovirus system has been developed. A recombinant adenovirus was constructed (expressing green fluorescent protein [GFP] under the control of the strong cytomegalovirus [CMV] promoter and a ...
Ann Vogel Hertzel, Mark A. Sanders, David A. Bernlohr   +2 more
doaj   +1 more source

Incorporation of reporter genes into mitochondrial DNA in budding yeast

STAR Protocols, 2022
Summary: Many aspects of mitochondrial gene expression are still unknown, which can be attributed to limitations in molecular tools. Here, we present a protocol to introduce reporter genes into the mitochondrial genome of budding yeast, Saccharomyces ...
Magdalena Rzepka, Tamara Suhm, Martin Ott   +2 more
doaj   +1 more source