Incorporation of reporter genes into mitochondrial DNA in budding yeast
STAR Protocols, 2022 Summary: Many aspects of mitochondrial gene expression are still unknown, which can be attributed to limitations in molecular tools. Here, we present a protocol to introduce reporter genes into the mitochondrial genome of budding yeast, Saccharomyces ...
Magdalena Rzepka +2 more
doaj +1 more source
Complex interactions between cis-regulatory modules in native conformation are critical for Drosophila snail expression [PDF]
, 2011 It has been shown in several organisms that multiple cis-regulatory modules (CRMs) of a gene locus can be active concurrently to support similar spatiotemporal expression.
Dunipace, Leslie +2 more
core +3 more sources
Assessment of a 44 gene classifier for the evaluation of chronic fatigue syndrome from peripheral blood mononuclear cell gene expression. [PDF]
PLoS ONE, 2011 Chronic fatigue syndrome (CFS) is a clinically defined illness estimated to affect millions of people worldwide causing significant morbidity and an annual cost of billions of dollars.
Daniel Frampton +3 more
doaj +1 more source
Frontiers in Cellular and Infection Microbiology, 2022 CRISPR editing has enabled the rapid creation of fluorescent Plasmodium transgenic lines, facilitating a deeper understanding of parasite biology. The impact of genetic perturbations such as gene disruption or the introduction of drug resistance alleles ...
Johanna Hoshizaki +2 more
doaj +1 more source
Imaging Molecular Pathways: Reporter Genes [PDF]
Radiation Research, 2012 Molecular imaging is a rapidly advancing field that allows cancer biologists to look deeper into the complex inner workings of tumor cells, or whole tumors, in a non-invasive manner. In this review, we will summarize some recent advances that enable investigators to study various important biological processes in tumors in vivo.
John, Brogan +5 more
openaire +2 more sources
Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls [PDF]
, 2007 Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid.
Klinghammer, Michaela, Tenhaken, Raimund
core +1 more source
Magnetic Resonance Reporter Gene Imaging [PDF]
Theranostics, 2012 Molecular imaging has undergone an explosive advancement in recent years, due to the tremendous research efforts made to understand and visualize biological processes. Molecular imaging by definition assesses cellular and molecular processes in living subjects, with the targets of following metabolic, genomic, and proteomic events.
Lee, Sheen-Woo +2 more
openaire +2 more sources
BioTechniques, 2000 Pathogenic microbes influence gene regulation in eukaryotic hosts. Reporter gene studies can define the roles of promoter regulatory sequences. The effect of pathogenic bacteria on reporter genes has not been examined.
Suzana D. Savkovic +3 more
doaj +1 more source
Preparation and application of chikungunya pseudovirus containing double reporter genes
Scientific Reports, 2022 Chikungunya virus (CHIKV), a highly infectious and rapidly spread viral pathogen, is classified as a pathogenic agent at the biosafety level 3. Operation of live authentic CHIKV needs a specific laboratory with the P3 or above containment, which greatly ...
Chunyan Su +7 more
doaj +1 more source
ArgR is an essential local transcriptional regulator of the arcABC-operon in Streptococcus suis and crucial for biological fitness in acidic environment [PDF]
, 2011 Streptococcus suis is one of the most important pathogens in pigs and can also cause severe infections in humans. Despite its clinical relevance very little is known about the factors contributing to its virulence.
Benga, L. +6 more
core +2 more sources