Tax4Fun: predicting functional profiles from metagenomic 16S rRNA data
Motivation: The characterization of phylogenetic and functional diversity is a key element in the analysis of microbial communities. Amplicon-based sequencing of marker genes, such as 16S rRNA, is a powerful tool for assessing and comparing the structure
K. Aßhauer +3 more
semanticscholar +1 more source
Study on rRNA Genes in Mycobacterium smegmatis [PDF]
AbstractThe number of rRNA genes in Mycobacterium smegmatis was examined by hybridization of BamHI and SalI digests of chromosomal DNA with 3′‐end‐labeled 5S, 16S, 23S rRNA and tRNA. Each RNA probe gave two hybridization bands. The PstI fragments of 6.6 kilobases were cloned to pBR322.
Y, Suzuki, T, Yamada
openaire +2 more sources
Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada +5 more
wiley +1 more source
Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu)
Some genes like 18S rRNA, 26S rRNA, elongation factor 1-alpha (EF1a), and beta-tubulin (TUB) are members of housekeeping genes group that are commonly used as internal control in gene expression study.
Dewi Indriyani Roslim +6 more
doaj +1 more source
Intra-Genomic Heterogeneity in 16S rRNA Genes in Strictly Anaerobic Clinical Isolates from Periodontal Abscesses. [PDF]
Members of the genera Prevotella, Veillonella and Fusobacterium are the predominant culturable obligate anaerobic bacteria isolated from periodontal abscesses.
Jiazhen Chen +8 more
doaj +1 more source
Effect of Pyrolysis on the Removal of Antibiotic Resistance Genes and Class I Integrons from Municipal Wastewater Biosolids [PDF]
Wastewater biosolids represent a significant reservoir of antibiotic resistance genes (ARGs). While current biosolids treatment technologies can reduce ARG levels in residual wastewater biosolids, observed removal rates vary substantially.
Kappell, Anthony D. +2 more
core +1 more source
Variation in hemolytic activity of Brachyspira hyodysenteriae strains from pigs [PDF]
Brachyspira hyodysenteriae is the primary cause of swine dysentery, which is responsible for major economic losses to the pig industry worldwide. The hemolytic activity of 10 B.
Boyen, Filip +8 more
core +4 more sources
A renaissance for the pioneering 16S rRNA gene [PDF]
Culture-independent molecular surveys using the 16S rRNA gene have become a mainstay for characterizing microbial community structure over the past quarter century. More recently this approach has been overshadowed by metagenomics, which provides a global overview of a community's functional potential rather than just an inventory of its inhabitants ...
Tringe, SG, Hugenholtz, P
openaire +3 more sources
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
The complete mitochondrial genome of Heniochus chrysostomus (Perciformes, Chaetodontidae)
We report the first mitochondrial genome sequences for the three band pennant fish, Heniochus chrysostomus. The whole mitogenome of H. chrysostomus was circular in shape and 16,650 bp in length.
Wei Yu +11 more
doaj +1 more source

