Results 121 to 130 of about 434,115 (269)

Expanding Genetic Code to Generate Human Brain Organoids with Both Vasculature and Microglia-Like Cells. [PDF]

Adv Sci (Weinh)
Lin H, Wang Y, Du H, Qin Y, Zhang H, Wang P, Wei L, Qin J.   +7 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Ribosome engineering enhances genetic code expansion in <i>Saccharomyces cerevisiae</i>. [PDF]

Synth Syst Biotechnol
Chen X, Shen W, Chen X, Zhang F, Yang B, Yue Z, Zhao G.   +6 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Development of m6A Readers for Enhanced Recognition In Vitro via Genetic Code Expansion. [PDF]

ACS Chem Biol
Addo M, Ficaretta E, Chang Y, Qiu W, Teng C, Bao JL, Chatterjee A, Zhou H.   +7 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Expanding the Genetic Code with Lysine Aminoacylation. [PDF]

J Am Chem Soc
Li X, Gan Q, Fan C.
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Punctuation in the Genetic Code

Cold Spring Harbor Symposia on Quantitative Biology, 1966
N D, Zinder, D L, Engelhardt, R E, Webster   +2 more
openaire   +2 more sources

Genetic Code Expanded T Cell for Controllable Immunotherapy. [PDF]

Adv Sci (Weinh)
Wang X, Gao Y, Su Y, Wang Y, Liu T.
europepmc   +1 more source