Results 161 to 170 of about 641,007 (266)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Insights into Genetic Diversity Within the Auliekol Cattle Breed Using SNP Genotyping. [PDF]

Animals (Basel)
Dossybayev K, Karymsakov T, Yergali K, Kozhakhmet A, Bekman D, Sailaubek P, Tapelov A, Ciani E, Torekhanov A, Bekmanov B.   +9 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Genetic diversity and geographical distribution of Trypanosoma cruzi DTUs in Mexico: A Systematic Review. [PDF]

Rev Soc Bras Med Trop
Cruz-Cadena RE, Velázquez-Ramírez DD, Fuentes-Vicente JA, Cruz-Trujillo R, Espinosa-Juárez JV, Cruz-Salomón A, Domínguez-Cruz DC.   +6 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Prevalence, Genetic Diversity, and Zoonotic Transmission Potential of Cryptosporidium spp. in Snakes: A Systematic Review and Meta-Analysis. [PDF]

Vet Med Sci
Asghari A, Pouryousef A, Karimi K, Kheiri A, Soltani MR, Maleki F.   +5 more
europepmc   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

Genetic Diversity Impacts Climate-Induced Species Range Shifts. [PDF]

Ecol Lett
Oliveira BF, Bertrand R, Comte L, Lenoir J, Grenouillet G, Lancaster LT, Murienne J, Diamond S, Scheffers BR, Bandara RMWJ, Lawlor JA, Moore NA, Wolfe BW, Villalobos F, Weiskopf SR, Thompson LM, Pinsky ML, Rolland J.   +17 more
europepmc   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Genetic Diversity Analysis of Red Fox Populations (Vulpes vulpes L., 1758) in Natural and Anthropogenic Isolation. [PDF]

Anim Genet
Hrebianchuk AE, Lukashkova VM, Parfionava NS, Ogden R, Tsybovsky IS.   +4 more
europepmc   +1 more source