Results 71 to 80 of about 4,912,059 (360)

Plant regeneration and Agrobacterium-mediated transformation of Achyranthes bidentata using cotton EREBP gene

Brazilian Archives of Biology and Technology, 2013
The aim of this work was to study the plant regeneration and Agrobacterium-mediated transformation of Achyranthes bidentata using cotton EREBP gene. Results showed that the callus induction rate of stems from A.
Hong Ying Duan, Xiao Sheng Ding, Jian Ying Song, Yun Long He, Yan Qing Zhou   +4 more
doaj   +1 more source

Agrobacterium-Mediated Stable Genetic Transformation of Populus angustifolia and Populus balsamifera

Frontiers in Plant Science, 2016
The present study demonstrates Agrobacterium tumefaciens-mediated stable genetic transformation of two species of poplar – Populus angustifolia and Populus balsamifera.
P. Maheshwari, I. Kovalchuk
semanticscholar   +1 more source

The carboxylate “gripper” of the substrate is critical for C‐4 stereo‐inversion by UDP‐glucuronic acid 4‐epimerase

FEBS Letters, EarlyView.
UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg, Laura De Cnop, Bernd Nidetzky   +2 more
wiley   +1 more source

A Cytotoxic and Anti-inflammatory Campesterol Derivative from Genetically Transformed Hairy Roots of Lopezia racemosa Cav. (Onagraceae)

Molecules, 2017
The genetically transformed hairy root line LRT 7.31 obtained by infecting leaf explants of Lopezia racemosa Cav with the Agrobacterium rhizogenes strain ATCC15834/pTDT, was evaluated to identify the anti-inflammatory and cytotoxic compounds reported ...
Norma Elizabeth Moreno-Anzúrez, Silvia Marquina, Laura Alvarez, Alejandro Zamilpa, Patricia Castillo-España, Irene Perea-Arango, Pilar Nicasio Torres, Maribel Herrera-Ruiz, Edgar Rolando Díaz García, Jaime Tortoriello García, Jesús Arellano-García   +10 more
doaj   +1 more source

TARRAGON (Artemisia dracunculus L.) “HAIRY” ROOT CULTURE PRODUCTION [PDF]

Biotechnologia Acta, 2016
This paper is devoted the biotechnology development of Artemisia dracunculus L. genetic transformation. We obtained the transgenic A. dracunculus “hairy” root culture using A. rhizogenes A4-mediated transformation.
K. O. , А. М. , N. A.
doaj   +1 more source

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Alteration of Panax ginseng saponin composition by overexpression and RNA interference of the protopanaxadiol 6-hydroxylase gene (CYP716A53v2)

Journal of Ginseng Research, 2016
Background: The roots of Panax ginseng contain noble tetracyclic triterpenoid saponins derived from dammarenediol-II. Dammarene-type ginsenosides are classified into the protopanaxadiol (PPD) and protopanaxatriol (PPT) groups based on their triterpene ...
Seong-Bum Park, Ju-Hyeon Chun, Yong-Wook Ban, Jung Yeon Han, Yong Eui Choi   +4 more
doaj   +1 more source

P‐glycoprotein modulates the fluidity gradient of the plasma membrane of multidrug resistant CHO cells

FEBS Letters, EarlyView.
To explore the impact of the overexpression of the multidrug‐transporter P‐glycoprotein (ABCB1) on membrane fluidity, we compared the transversal gradient of mobility and microviscosity in plasma membranes of drug‐sensitive Chinese hamster ovary cells (AuxB1) and their multidrug‐resistant derivatives (B30) using the fluorescent n‐(9‐anthroyloxy) fatty ...
Roger Busche, John R. Riordan, Burkhard Tümmler   +2 more
wiley   +1 more source

Overexpression of RsMYB1 Enhances Anthocyanin Accumulation and Heavy Metal Stress Tolerance in Transgenic Petunia

Frontiers in Plant Science, 2018
The RsMYB1 transcription factor (TF) controls the regulation of anthocyanin in radishes (Raphanus sativus), and its overexpression in tobacco and petunias strongly enhances anthocyanin production.
Trinh Ngoc Ai, Trinh Ngoc Ai, Aung Htay Naing, Byung-Wook Yun, Sun Hyung Lim, Chang Kil Kim   +5 more
doaj   +1 more source

Nanoparticle-mediated gene transformation strategies for plant genetic engineering.

The Plant Journal, 2020
Plant genetic engineering, a recent technological advancement in the field of plant science, is an important tool used to improve crop quality and increase yield, to enhance secondary metabolite content in medicinal plants, or to develop crops for ...
Zongyou Lv, Rui Jiang, Jun-feng Chen, Wansheng Chen   +3 more
semanticscholar   +1 more source