Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy
Molecular Oncology, EarlyView.We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley +1 more source
Grading, Minimum Quality Standards, and the Labeling of Genetically Modified Products [PDF]
We relate the labeling of genetically modified (GM) products to the theory of grading and minimum quality standards. The model represents three stages in the supply chain, assumes a vertical product differentiation framework, allows for the accidental ...
Lapan, Harvey E., Moschini, GianCarlo
core
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
FEBS Open Bio, EarlyView.Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
A Mixture Model of Consumers' Intended Purchase Decisions for Genetically Modified Foods [PDF]
A finite probability mixture model is used to analyze the existence of multiple market segments for a pre-market good. The approach has at least two principal benefits.
Kristine M. Grimsrud +2 more
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Genetically Modified and Gene-Edited Organisms-Objectives, Public Perception and Applications. [PDF]
Biology (Basel)Solti L, Fébel H.
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
EU regulation concerning genetically modified products: an issue of food security or a measure of disguised protectionism? [PDF]
The biggest producers and exporters of agricultural products have been adopting the genetic engineering in order to improve the factors productivity and the firms profits In the last decade, the United States of America (US) and the European Union (EU ...
Ana Santos, Jose Caetano
core
Genetically Modified Plants in Agriculture. [PDF]
Biology (Basel)Ogienko AA, Surkova ES, Omelina ES.
europepmc +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
MEASURING U.S. CONSUMER PREFERENCES FOR GENETICALLY MODIFIED FOODS USING CHOICE MODELING EXPERIMENTS: THE ROLE OF PRICE, PRODUCT BENEFITS AND TECHNOLOGY [PDF]
Food biotechnology promises to deliver a wide range of enhanced consumer benefits. This study models consumer's willingness to trade-off the potential risks of GM foods with the possibility of extracting significant benefits.
Onyango, Benjamin M. +2 more
core