Efficient DNA knock-in using AAV-mediated delivery with 2-cell embryo CRISPR-Cas9 electroporation
Frontiers in Genome Editing, 2023 Recent advances in CRISPR-Cas genome editing technology have been instrumental in improving the efficiency to produce genetically modified animal models.
Daniel J. Davis +6 more
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GABAergic compensation in connexin36 knock-out mice evident during low-magnesium seizure-like event activity [PDF]
, 2010 Gap junctions within the cerebral cortex may facilitate cortical seizure formation by their ability to synchronize electrical activity. To investigate this, one option is to compare wild-type (WT) animals with those lacking the gene for connexin36 (Cx36 ...
Jacobson, Gregory M. +3 more
core +2 more sources
Genetic Modifiers of Retinal Degeneration in therd3Mouse [PDF]
Investigative Opthalmology & Visual Science, 2008 In previous studies of light-induced (LRD) and age-related (ageRD) retinal degeneration (RD) between the BALB/cByJ (BALB) and B6(Cg)-Tyr(c-2J)/J (B6a) albino mouse strains, RD-modifying quantitative trait loci (QTLs) were identified. After breeding BALB- and B6a-rd3/rd3 congenic strains and finding significant differences in RD, an F1 intercross to ...
Michael, Danciger +8 more
openaire +2 more sources
Genetically Encoded Calcium Indicators: A New Tool in Renal Hypertension Research [PDF]
, 2019 Hypertension is ranked as the third cause of disability-adjusted life-years. The percentage of the population suffering from hypertension will continue to increase over the next years.
Schleifenbaum, Johanna, Zhong, Cheng
core +1 more source
Genetic platelet depletion is superior in platelet transfusion compared to current models
Haematologica, 2020 Genetically modified mice have advanced our knowledge on platelets in hemostasis and beyond tremendously. However, mouse models harbor certain limitations, including availability of platelet specific transgenic strains, and off-target effects on other ...
Manuel Salzmann +11 more
doaj +1 more source
Cancer immunoprevention: from mice to early clinical trials
BMC Immunology, 2018 Cancer immunoprevention is based on the fact that a functioning immune system controls tumor onset and development in humans and animals, thus leading to the idea that the enhancement of immune responses in healthy individuals could effectively reduce ...
Arianna Palladini +3 more
doaj +1 more source
An open-source video tracking system for mouse locomotor activity analysis
BMC Research Notes, 2020 Objective The ability to accurately and efficiently quantify mouse locomotor activity is essential for evaluating therapeutic efficacy and phenotyping genetically modified mice, in particular for the research of neuromuscular diseases.
Chen Zhang, Haiwen Li, Renzhi Han
doaj +1 more source
Molecular Therapy: Oncolytics, 2022 Malignant soft tissue tumors, particularly highly malignant leiomyosarcomas, are resistant to chemotherapy and associated with a poor prognosis. T-01, a third-generation genetically modified herpes simplex virus type 1, replicates in tumor cells alone ...
Masahiko Hatta +8 more
doaj +1 more source
Engineered Tumor-Targeted T Cells Mediate Enhanced Anti-Tumor Efficacy Both Directly and through Activation of the Endogenous Immune System. [PDF]
, 2018 Chimeric antigen receptor (CAR) TÂ cell therapy has proven clinically beneficial against B cell acute lymphoblastic leukemia and non-Hodgkin's lymphoma. However, suboptimal clinical outcomes have been associated with decreased expansion and persistence of
Avanzi, Mauro P +10 more
core +1 more source
Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice
Asian Journal of Andrology, 2014 The blood-testis barrier (BTB) is found between adjacent Sertoli cells in the testis where it creates a unique microenvironment for the development and maturation of meiotic and postmeiotic germ cells in seminiferous tubes.
Xiao-Hua Jiang +6 more
doaj +1 more source