Results 61 to 70 of about 390,635 (308)

sgRNA Knock-in Mouse Provides an Alternative Approach for In Vivo Genetic Modification

Frontiers in Cell and Developmental Biology, 2022
Functional genomics in a mammalian model such as mice is fundamental for understanding human biology. The CRISPR/Cas9 system dramatically changed the tempo of obtaining genetic mouse models due to high efficiency.
Lichen Zhang, Lichen Zhang, Wushan Li, Wushan Li, Zhuangzhuang Liu, Zhuangzhuang Liu, Yang Liu, Yang Liu, Zhilong Liu, Zhilong Liu, Yanrong Gu, Yanrong Gu, Le He, Le He, Binhui Zhou, Binhui Zhou, Tianhan Li, Tianhan Li, Tianzhu Chao, Tianzhu Chao, Yinming Liang, Yinming Liang, Yinming Liang, Liaoxun Lu, Liaoxun Lu, Liaoxun Lu   +25 more
doaj   +1 more source

Gabra2 is a genetic modifier of Scn8a encephalopathy in the mouse* [PDF]

Epilepsia, 2020
AbstractObjectiveSCN8A encephalopathy is a developmental epileptic encephalopathy typically caused by de novo gain‐of‐function mutations in Nav1.6. Severely affected individuals exhibit refractory seizures, developmental delay, cognitive disabilities, movement disorders, and elevated risk of sudden death.
Wenxi Yu, Sophie F. Hill, James G. Xenakis, Fernando Pardo‐Manuel de Villena, Jacy L. Wagnon, Miriam H. Meisler   +5 more
openaire   +2 more sources

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Development of HPV16 mouse and dog models for more accurate prediction of human vaccine efficacy

Laboratory Animal Research, 2023
Background Animal models are essential to understand the physiopathology of human diseases but also to evaluate new therapies. However, for several diseases there is no appropriate animal model, which complicates the development of effective therapies ...
Emmanuelle Totain, Loïc Lindner, Nicolas Martin, Yolande Misseri, Alexandra Iché, Marie-Christine Birling, Tania Sorg, Yann Herault, Alain Bousquet-Melou, Pascale Bouillé, Christine Duthoit, Guillaume Pavlovic, Severine Boullier   +12 more
doaj   +1 more source

Factors controlling sperm migration through the oviduct revealed by gene-modified mouse models

Experimental animals, 2018
Mammalian fertilization is comprised of many steps including sperm survival in the uterus, sperm migration in the female reproductive tract, physiological and morphological changes to the spermatozoa, and sperm-egg interaction in the oviduct.
Yoshitaka Fujihara, H. Miyata, M. Ikawa
semanticscholar   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain

Journal of Neuroinflammation, 2020
Background A growing body of studies have indicated that bone marrow mesenchymal stem cells (BMSCs) have powerful analgesic effects in animal models of bone cancer pain.
Chao Zhu, Kun Wang, Zhi Chen, Yingchao Han, Hao Chen, Quan Li, Zude Liu, Lie Qian, Jun Tang, Hongxing Shen   +9 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

FEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino, Meijie Li, Cécile Fizames, Mattia Adamo, Laurence Lejay, Christian Dubos, Matthieu Platre, Antoine Martin   +7 more
wiley   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source