Results 31 to 40 of about 125,982 (264)

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Competition between homologous chromosomal DNA and exogenous donor DNA to repair CRISPR/Cas9-induced double-strand breaks in Aspergillus  niger

Fungal Biology and Biotechnology
Background Aspergillus niger is well-known for its high protein secretion capacity and therefore an important cell factory for homologous and heterologous protein production.
Selina Forrer, Mark Arentshorst, Prajeesh Koolth Valappil, Jaap Visser, Arthur F. J. Ram   +4 more
doaj   +1 more source

The Dynamic Transition of Persistence toward the Viable but Nonculturable State during Stationary Phase Is Driven by Protein Aggregation

mBio, 2021
Decades of research into bacterial persistence has been unable to fully characterize this antibiotic-tolerant phenotype, thereby hampering the development of therapies effective against chronic infections.
Liselot Dewachter, Celien Bollen, Dorien Wilmaerts, Elen Louwagie, Pauline Herpels, Paul Matthay, Ladan Khodaparast, Laleh Khodaparast, Frederic Rousseau, Joost Schymkowitz, Jan Michiels   +10 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Bioconversion of biphenyl to a polyhydroxyalkanoate copolymer by Alcaligenes denitrificans A41

AMB Express, 2020
A polyhydroxyalkanoate (PHA) copolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], was biosynthesized from biphenyl as the sole carbon source using Alcaligenes (currently Achromobacter) denitrificans A41.
Taito Yajima, Mizuki Nagatomo, Aiko Wakabayashi, Michio Sato, Seiichi Taguchi, Michihisa Maeda   +5 more
doaj   +1 more source

LATS kinases and SLUG regulate the transition to advanced stage in aggressive oral cancer cells

Scientific Reports, 2022
The epithelial-to-mesenchymal transition (EMT) is a critical process by which cancer cells acquire malignant features. However, the molecular mechanism and functional implications of EMT and the mesenchymal-to-epithelial transition (MET) in tumor ...
Emi Fujibayashi, Satomi Mukai, Kosuke Torigata, Yumi Ando, Toshihiro Uchihashi, Masami Nozaki, Susumu Tanaka, Masato Okada, Mikihiko Kogo, Hiroshi Nojima, Norikazu Yabuta   +10 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Salmonella stress response systems as targets for anti-virulence strategies

BMC Microbiology
Due to the rapid development of antibiotic resistance, novel strategies to cope with bacterial infections are urgently required. Ideally, these strategies should exert less selection pressure for resistance than traditional antibiotics.
Alshimaa Hassanin, Bram Lories, Hans P. Steenackers   +2 more
doaj   +1 more source

Imidazoles and Quaternary Ammonium Compounds as Effective Therapies against (Multidrug-Resistant) Bacterial Wound Infections

Antibiotics
Background/Objectives: The rise and spread of antimicrobial resistance complicates the treatment of bacterial wound pathogens, further increasing the need for newer, effective therapies.
Lauren Van de Vliet, Thijs Vackier, Karin Thevissen, David Decoster, Hans P. Steenackers   +4 more
doaj   +1 more source