Results 51 to 60 of about 2,058,071 (297)
BoostMe accurately predicts DNA methylation values in whole-genome bisulfite sequencing of multiple human tissues
BMC Genomics, 2018 Background Bisulfite sequencing is widely employed to study the role of DNA methylation in disease; however, the data suffer from biases due to coverage depth variability.Luli S. Zou, Michael R. Erdos, D. Leland Taylor, Peter S. Chines, Arushi Varshney, The McDonnell Genome Institute, Stephen C. J. Parker, Francis S. Collins, John P. Didion +8 moredoaj +1 more sourceScreening of the SHOX/PAR1 region using MLPA and miRNA expression profiling in a group of Egyptian children with non-syndromic short stature
Egyptian Journal of Medical Human Genetics, 2020 Background Short stature is defined as a body height below the third percentile, based on chronological age, or 2 standard deviations (SD) below the national height standard. The prevalence of short stature is around 2% of children worldwide.Ola M. Eid, Maha M. Eid, Amany H. Abdelrahman, Rania M. A. Abdel kader, Marwa Farid, Rana Mahrous, Mona K. Mekkawy, Alaa K. Kamel, Inas Mazen, Hala T. El-Bassyouni +9 moredoaj +1 more sourceHyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky +11 morewiley +1 more sourcePre-sequencing assessment of RNA-Seq library quality using real-time qPCR
BioTechniquesRNA sequencing (RNA-Seq) is an essential assay for studying transcriptome profiling. Ribosomal RNA (rRNA) comprises more than 80–90% of total cellular RNA; efficient removal is essential for accurately capturing transcriptomes, particularly to sequence ...Kavya Kottapalli, Hsu Chao, Qi Jiang, Jimmonique Donelson, Nathanael Emerick, Sravya V Bhamidipati, Zeineen Momin, Tittu Mathew, Ziad M. Khan, Viktoriya Korchina, Marie-Claude Gingras, Donna Muzny, Richard Gibbs, Harsha Doddapaneni +13 moredoaj +1 more sourceGenome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli [PDF]
, 2014 Background: Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America.A Buschiazzo, AC Frasch, AC Ivens, AL Price, Alexandra Gerber, Ana Tereza Ribeiro de Vasconcelos, Arnaldo Zaha, B Li, B Roure, Björn Andersson, C Biemont, C Folgueira, CA Buscaglia, CA Buscaglia, Carlos Talavera-Lopez, CB Lira, CB Toaldo, CE Butler, Claudia Elizabeth Thompson, CP Pena, CS Peacock, D Bahia, D Bahia, D Cosentino-Gomes, D Ekanayake, D Horn, D Miranda-Saavedra, D Salmon, DA Largaespada, DA Urrea, DA Urrea, Daniella Castanheira Bartholomeu, DC Bartholomeu, DG Passos-Silva, Diana Bahia, DM Martin, Débora Denardin Lückemeyer, E Ghedin, EC Grisard, EC Grisard, Edmundo Carlos Grisard, EJ Tobie, EL Raven, Elgion Loreto, Elisa Beatriz Prestes, F Corpet, F Guhl, F Guhl, F Maia Da Silva, F Maia da Silva, F Maia da Silva, F Sievers, FB Nogueira, Fábio Mitsuo Lima, G Benson, G Wagner, GA Vallejo, GA Vallejo, GA Vallejo, Gabriela Rodrigues-Luiz, Glauber Wagner, Gustavo Adolfo Vallejo, H Castro, H Diez, H Diez, H Diez, H Ngo, H Shi, H Shi, HA Schmidt, HG van Luenen, HS Kim, I Kamileri, I Romero, J Baum, J Fonager, J Henriksson, J Jurka, JD Damasceno, JE Vasquez, Jessica C. Kissinger, JF Turrens, JH Lee, JL Affranchino, JL Parsons, José Franco da Silveira Filho, JV Bannister, K Ersfeld, K Strimmer, KA Norris, Karina Mariante Monteiro, Kevin Morris Tyler, KL Patrick, L Piacenza, L Piacenza, LF Lye, LG Almeida, LJ Cliffe, LM Freitas, Luiz Gonzaga Paula de Almeida, M Berriman, M Cabrine-Santos, M Cabrine-Santos, M Schramp, M Steindel, MA Chiurillo, MA de Sousa, Mauro Freitas Ortiz, MB Rogers, MC Elias, MC Motta, MD Pineyro, MF Amaya, MH de Moraes, MH Saier Jr, MI Cano, Miguel Angel Chiurillo, Milene Höehr de Moraes, MJ Fraser Jr, MJ Schofield, MM Kangussu-Marcolino, MP Wymann, MR Briones, MR Garcia Silva, MT Tammi, Mário Steindel, N Anez-Rojas, N Añez, N Inoue, N Saitou, NM El-Sayed, NM El-Sayed, O Franzen, O Franzen, Oberdan de Lima Cunha, P Luciano, Patrícia Hermes Stoco, R Bosotti, R Marone, RD Page, RL Barnes, Rondon Mendonça-Neto, Rosane Silva, RR Moraes Barros, RT Souza, S Cestari Idos, S Kumar, S Martinez-Calvillo, S Muller, S Schenkman, Santuza Maria Ribeiro Teixeira, SF Altschul, Silvane Maria Fonseca Murta, SJ Westenberger, SL dos Santos, SM Teixeira, SR Wilkinson, SR Wilkinson, SR Wilkinson, SS Dc-Rubin, Sérgio Schenkman, T Downing, TA Minning, TA Pitcovsky, Thais Cristine Marques Sincero, Tiago Antonio de Oliveira Mendes, Turán Peter Urmenyi, Viviane Grazielle Silva, Wanderson Duarte DaRocha, WD DaRocha, X Huang, ZC Caballero, Álvaro José Romanha +172 morecore +3 more sourcesThe neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy
Molecular Oncology, EarlyView.ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal +8 morewiley +1 more sourceIn vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations
Molecular Oncology, EarlyView.Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow +10 morewiley +1 more sourceTranscriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication
Molecular Oncology, EarlyView.Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal +16 morewiley +1 more source
