Results 71 to 80 of about 1,632,775 (273)

Template-based eukaryotic genome editing directed by SviCas3 [PDF]

arXiv, 2023
RNA-guided gene editing based on the CRISPR-Cas system is currently the most effective genome editing technique. Here, we report that the SviCas3 from the subtype I-B-Svi Cas system in Streptomyces virginiae IBL14 is an RNA-guided and DNA-guided DNA endonuclease suitable for the HDR-directed gene and/or base editing of eukaryotic cell genomes.
arxiv  

Differentially activated B cells develop regulatory phenotype and show varying immunosuppressive features: a comparative study

Frontiers in Immunology, 2023
Regulatory B lymphocytes (Bregs) are B cells with well-pronounced immunosuppressive properties, allowing them to suppress the activity of effector cells.
Elina A. Zheremyan, Alina S. Ustiugova, Aksinya N. Uvarova, Nina M. Karamushka, Ekaterina M. Stasevich, Violetta S. Gogoleva, Apollinariya V. Bogolyubova, Nikita A. Mitkin, Dmitry V. Kuprash, Dmitry V. Kuprash, Kirill V. Korneev, Kirill V. Korneev   +11 more
doaj   +1 more source

Microbial Genome Editing

, 2023
Creative Biogene has the capacity to specifically modify microbial genomes in an efficient and precise way. With talented staffs, advanced technologies and state-of-the-art facilities, Creative Biogene provides microbial genome editing service, including traditional recombineering system, CRISPR/Cas 9 system and site-specific recombination system.
openaire   +2 more sources

Breakthrough to genome editing [PDF]

Science, 2015
A, T, G, C: the alphabet code for the nucleotides that are the building blocks of life. Minor, but consequential, changes in this DNA coding can change gene function. Researchers have long sought better ways to edit the genetic code in cultured cells and laboratory organisms to silence, activate, or change targeted genes to gain a better ...
openaire   +3 more sources

Editing the Mitochondrial Genome

New England Journal of Medicine, 2020
Mending Mitochondrial Mutation For most patients with mitochondrial disorders, most of which are caused by mutations in genes in the mitochondrial genome, only symptomatic treatment is available.
Maria Falkenberg, Michio Hirano
openaire   +3 more sources

Is there any sense in antisense editing? [PDF]

Trends in Genetics 21, 544-7 (2005), 2005
A number of recent studies have hypothesized that sense-antisense RNA transcript pairs create dsRNA duplexes that undergo extensive A-to-I RNA editing. Here we studied human and mouse genomic antisense regions, and found that the editing level in these areas is negligible.
arxiv   +1 more source

Whole genome MBD-seq and RRBS analyses reveal that hypermethylation of gastrointestinal hormone receptors is associated with gastric carcinogenesis

Experimental and Molecular Medicine, 2018
Stomach cancer: DNA methylation of hormone receptor genes A sequencing study reveals abnormal changes to DNA that set the stage for stomach cancer development.
Hee-Jin Kim, Tae-Wook Kang, Keeok Haam, Mirang Kim, Seon-Kyu Kim, Seon-Young Kim, Sang-Il Lee, Kyu-Sang Song, Hyun-Yong Jeong, Yong Sung Kim   +9 more
doaj   +1 more source

Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing

Frontiers in Genetics, 2021
The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises
Lijuan Duan, Kan Ouyang, Xiao Xu, Limei Xu, Caining Wen, Xiaoying Zhou, Zhuan Qin, Zhiyi Xu, Wei Sun, Yujie Liang   +9 more
semanticscholar   +1 more source

Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]

, 2018
Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson, Andersson, Cho, Clasen, Eriksson, Gorbunova, Hartung, Jinek, Jones, Kim, Kuipers, Kuipers, Larkin, Liang, Muthoni, Nelson, Sawai, Shepard, Svitashev, Visser, Woo, Xu, Zhang   +22 more
core   +1 more source

Engineered extracellular vesicles as versatile ribonucleoprotein delivery vehicles for efficient and safe CRISPR genome editing

Journal of Extracellular Vesicles, 2021
Transient delivery of CRISPR‐based genome editing effectors is important to reduce off‐target effects and immune responses. Recently extracellular vesicles (EVs) have been explored for Cas9 ribonucleoprotein (RNP) delivery. However, lack of mechanisms to
Xingang Yao, Pin Lyu, K. Yoo, M. Yadav, Ravi Singh, A. Atala, B. Lu   +6 more
semanticscholar   +1 more source