Results 71 to 80 of about 571,422 (359)
FEBS Letters, EarlyView.In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi +5 more
wiley +1 more source
Microorganisms, 2023 The aim of this work was to study the genetic diversity of the gonococcal genetic island (GGI) responsible for the type IV secretion system (T4SS) and the association of a functionally active GGI with antimicrobial resistance. An analysis of the GGI in a
Dmitry Kravtsov +2 more
doaj +1 more source
Genome Editing – Interdisziplinäre Technikfolgenabschätzung [PDF]
, 2019 ISBN:978-3-7281-3981 ...
Lang, Alexander +11 more
openaire +11 more sources
From omics to AI—mapping the pathogenic pathways in type 2 diabetes
FEBS Letters, EarlyView.Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan +2 more
wiley +1 more source
Development of visible light‐sensitive human neuropsin (OPN5) via single amino acid substitution
FEBS Letters, EarlyView.The present study determines a key amino acid residue, Lys91, for defining UV sensitivity of human OPN5. Heterologous action spectroscopy of the wild type and K91 mutants of OPN5 in HEK293T cells reveals that substitution of Lys91 with neutral (alanine) or acidic amino acids (glutamic or aspartic acids) causes substantial shifts in spectral sensitivity
Yusuke Sakai +2 more
wiley +1 more source
PEM-seq comprehensively quantifies DNA repair outcomes during gene-editing and DSB repair
STAR Protocols, 2022 Summary: The repair products of double-stranded DNA breaks (DSBs) are crucial for investigating the mechanism underlying DNA damage repair as well as evaluating the safety and efficiency of gene-editing; however, a comprehensively quantitative assay ...
Yang Liu +6 more
doaj +1 more source
Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, Aedes aegypti. [PDF]
, 2017 The development of CRISPR/Cas9 technologies has dramatically increased the accessibility and efficiency of genome editing in many organisms. In general, in vivo germline expression of Cas9 results in substantially higher activity than embryonic injection.
Akbari, Omar S +5 more
core +1 more source
History of genome editing in yeast [PDF]
Yeast, 2018 AbstractFor thousands of years humans have used the budding yeast Saccharomyces cerevisiae for the production of bread and alcohol; however, in the last 30–40 years our understanding of the yeast biology has dramatically increased, enabling us to modify its genome. Although S.
Marcin G. Fraczek +2 more
openaire +3 more sources
Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells
FEBS Letters, EarlyView.Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan +2 more
wiley +1 more source
ErCas12a and T5exo-ErCas12a Mediate Simple and Efficient Genome Editing in Zebrafish
Biology, 2022 In zebrafish, RNA-guided endonucleases such as Cas9 have enabled straightforward gene knockout and the construction of reporter lines or conditional alleles via targeted knockin strategies. However, the performance of another commonly used CRISPR system,
Bingzhou Han +8 more
doaj +1 more source