Results 101 to 110 of about 3,929,879 (390)

Two Distinct Pathways Support Gene Correction by Single-Stranded Donors at DNA Nicks

Cell Reports, 2016
Nicks are the most common form of DNA damage. The mechanisms of their repair are fundamental to genomic stability and of practical importance for genome engineering.
Luther Davis, Nancy Maizels
doaj   +1 more source

A Toolkit for Effective and Successive Genome Engineering of Escherichia coli

Fermentation, 2022
The bacterium Escherichia coli has been well-justified as an effective workhorse for industrial applications. In this study, we developed a toolkit for flexible genome engineering of this microorganism, including site-specific insertion of heterologous ...
Bahareh Arab, Adam Westbrook, Murray Moo-Young, Chih-Hsiung Perry Chou   +3 more
doaj   +1 more source

An enhanced CRISPR repressor for targeted mammalian gene regulation. [PDF]

, 2018
The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility.
Cecchi, Ryan J, Chan, Yingleong, Chavez, Alejandro, Church, George M, Church, George M, Collins, James J, Davidsohn, Noah, Ebrahimkhani, Mo R, Guo, Xiaoge, Kiani, Samira, Kuo, Chih-Chung, Lance-Byrne, Alissa, Lewis, Nathan E, Lim, Elaine T, Menn, David, Milanova, Denitsa, Pradhan, Swechchha, Sharma, Sumana, Tung, Angela, Tuttle, Marcelle, Yeo, Nan Cher   +20 more
core   +1 more source

Enhancing phage therapy through synthetic biology and genome engineering

Current Opinion in Biotechnology, 2020
The antimicrobial and therapeutic efficacy of bacteriophages is currently limited, mostly due to rapid emergence of phage-resistance and the inability of most phage isolates to bind and infect a broad range of clinical strains. Here, we discuss how phage
B. R. Lenneman, Jonas Fernbach, M. Loessner, T. Lu, S. Kilcher   +4 more
semanticscholar   +1 more source

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source

Geminiviruses for biotechnology: the art of parasite taming [PDF]

, 2017
ConferenciaViruses are intracellular pathogens that have evolved efficient strategies for replication and expression of their proteins in the host cells.
Lozano Duran, Rosa
core  

Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice

Communications Biology, 2020
Precise genome editing by systems such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) requires high-efficiency homology-directed repair (HDR). Different technologies have been developed to improve
Zahir Ali, A. Shami, Khalid Sedeek, Radwa Kamel, Abdulrahman Alhabsi, M. Tehseen, Norhan Hassan, Haroon Butt, A. Kababji, S. Hamdan, Magdy M. Mahfouz   +10 more
semanticscholar   +1 more source

Organoid technologies meet genome engineering [PDF]

EMBO reports, 2017
Three-dimensional (3D) stem cell differentiation cultures recently emerged as a novel model system for investigating human embryonic development and disease progression in vitro, complementing existing animal and two-dimensional (2D) cell culture models.
Jing Nie, Eri Hashino
openaire   +2 more sources

PARP inhibition and pharmacological ascorbate demonstrate synergy in castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
Pharmacologic ascorbate (vitamin C) increases ROS, disrupts cellular metabolism, and induces DNA damage in CRPC cells. These effects sensitize tumors to PARP inhibition, producing synergistic growth suppression with olaparib in vitro and significantly delayed tumor progression in vivo. Pyruvate rescue confirms ROS‐dependent activity.
Nicolas Gordon, Peter T. Gallagher, Orly I. Richter, Neermala Poudel Neupane, Amy C. Mandigo, Jennifer J. McCann, Emanuela Dylgjeri, Irina Vasilevskaya, Christopher McNair, Channing J. Paller, Wm. Kevin Kelly, Karen E. Knudsen, Matthew J. Schiewer, Ayesha A. Shafi   +13 more
wiley   +1 more source

CRISPR/Cas9-mediated genome engineering and the promise of designer flies on demand

Fly, 2013
The CRISPR/Cas9 system has attracted significant attention for its potential to transform genome engineering. We and others have recently shown that the RNA-guided Cas9 nuclease can be employed to engineer the Drosophila genome, and that these ...
Scott J Gratz, Jill Wildonger, Melissa M Harrison, Kate M O'Connor-Giles   +3 more
doaj   +1 more source