Results 81 to 90 of about 3,929,879 (390)

Artificial and Natural Genetic Information Processing [PDF]

, 2017
Conventional methods of genetic engineering and more recent genome editing techniques focus on identifying genetic target sequences for manipulation.
Witzany, Guenther
core  

MultiMetEval: comparative and multi-objective analysis of genome-scale metabolic models [PDF]

, 2012
Comparative metabolic modelling is emerging as a novel field, supported by the development of reliable and standardized approaches for constructing genome-scale metabolic models in high throughput.
A Freitag, A Gevorgyan, A Mithani, Albert Gevorgyan, AM Feist, AM Feist, AM Feist, Andrzej M. Kierzek, AP Burgard, BA Pfeifer, CS Henry, D Nagrath, DC Alexander, DC Stevens, EM Paradise, Eriko Takano, FC Lee, H Ikeda, I Thiele, J Gonzalez-Lergier, J Romero, J Schellenberger, JD Orth, JL Adrio, JP Brooks, JS Edwards, K Nieselt, K Yanai, LM Blank, M DeJongh, M Durot, M Kanehisa, M Latendresse, MA Fischbach, MA Oberhardt, MA Oberhardt, MA Oberhardt, Marnix H. Medema, MH Medema, MH Medema, MH Medema, MH Medema, MT Alam, MT Alam, MW Covert, N Scherr, ND Price, ND Price, NE Lewis, Piotr Zakrzewski, PR Ives, R Breitling, R Schuetz, Rainer Breitling, SA Becker, SD Bentley, Stephen S. Fong, TD Vo, TY Kim, XQ Zhao   +59 more
core   +6 more sources

Precision genome engineering [PDF]

Science, 2018
Biotechnology Genome editing through CRISPR-Cas systems has the potential to correct genetic mutations that occur in diseased cells, such as cancer cells. However, the ability to selectively activate CRISPR-Cas systems in diseased cells is important to ensure that gene editing only occurs where it is wanted. Zhu et al.
openaire   +1 more source

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis

Cell Reports, 2017
We analyzed chromatin dynamics and transcriptional activity of human embryonic stem cell (hESC)-derived cardiac progenitor cells (CPCs) and KDR+/CD34+ endothelial cells generated from different mesodermal origins.
Nathan J. Palpant, Yuliang Wang, Brandon Hadland, Rebecca J. Zaunbrecher, Meredith Redd, Daniel Jones, Lil Pabon, Rajan Jain, Jonathan Epstein, Walter L. Ruzzo, Ying Zheng, Irwin Bernstein, Adam Margolin, Charles E. Murry   +13 more
doaj   +1 more source

Precise genome engineering in Drosophila using prime editing

Proceedings of the National Academy of Sciences of the United States of America, 2020
Significance Precise genome engineering allows researchers to modify gene function, tag endogenous proteins, or model human disease mutations. Here, we adapt prime editing, a new CRISPR-based technology that uses reverse transcription to write precise ...
Justin A. Bosch, Gabriel Birchak, N. Perrimon   +2 more
semanticscholar   +1 more source

RELATe enables genome-scale engineering in fungal genomics [PDF]

Science Advances, 2020
Genomic DNA can be converted into sgRNA libraries for genome-wide screening of fungal factors in blood-brain barrier penetration.
Zhongming Li, Kwang Sik Kim
openaire   +2 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

CRISPR/Cas9 for Cancer Therapy: Hopes and Challenges

Biomedicines, 2018
Cancer is the second leading cause of death globally and remains a major economic and social burden. Although our understanding of cancer at the molecular level continues to improve, more effort is needed to develop new therapeutic tools and approaches ...
Marta Martinez-Lage, Pilar Puig-Serra, Pablo Menendez, Raul Torres-Ruiz, Sandra Rodriguez-Perales   +4 more
doaj   +1 more source

Nanoparticle analysis sheds budding insights into genetic drivers of extracellular vesicle biogenesis [PDF]

Journal of Extracellular Vesicles, 2016
Background: Extracellular vesicles (EVs) are important mediators of cell-to-cell communication in healthy and pathological environments. Because EVs are present in a variety of biological fluids and contain molecular signatures of their cell or tissue of
Stephanie N. Hurwitz, Meghan M. Conlon, Mark A. Rider, Naomi C. Brownstein, David G. Meckes   +4 more
doaj   +1 more source