Results 111 to 120 of about 3,929,879 (390)

Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida

Metabolic Engineering Communications, 2020
Genome engineering of non-conventional microorganisms calls for the development of dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium widely used for metabolic engineering owing to its versatile ...
D. Volke, Laura Friis, Nicolas T. Wirth, Justine Turlin, P. Nikel   +4 more
semanticscholar   +1 more source

Plecstatin inhibits hepatocellular carcinoma tumorigenesis and invasion through cytolinker plectin

Molecular Oncology, EarlyView.
The ruthenium‐based metallodrug plecstatin exerts its anticancer effect in hepatocellular carcinoma (HCC) primarily through selective targeting of plectin. By disrupting plectin‐mediated cytoskeletal organization, plecstatin inhibits anchorage‐dependent growth, cell polarization, and tumor cell dissemination.
Zuzana Outla, Jan Kosla, Magdalena Prechova, Lukas Frick, Patricia Bortel, Yasmin Borutzki, Andrea Bileck, Christopher Gerner, Samuel M. Meier‐Menches, Selma Osmanagic‐Myers, Martin Gregor   +10 more
wiley   +1 more source

Reprogramming human T cell function and specificity with non-viral genome targeting. [PDF]

, 2018
Decades of work have aimed to genetically reprogram T cells for therapeutic purposes1,2 using recombinant viral vectors, which do not target transgenes to specific genomic sites3,4.
Alkorta-Aranburu, Gorka, Ashworth, Alan, Bacchetta, Rosa, Carmody, David, Carnevale, Julia, Chen, Jeff W, Cho, Min, Del Gaudio, Daniela, Esensten, Jonathan H, Ferris, Andrea L, Greeley, Siri Atma W, Gurumurthy, Channabasavaiah B, Haugwitz, Michael, Herold, Kevan C, Hiatt, Joseph, Hughes, Stephen H, Krystofinski, Paige, Kupfer, Gary M, Lee, Michael R, Leonetti, Manuel D, Li, Han, Li, P Jonathan, Mao, Ying, Marson, Alexander, Matsumoto, Hiroyuki, May, Andrew P, Meffre, Eric, Morell, Montse, Nguyen, David N, Pellerin, Laurence, Puig-Saus, Cristina, Putnam, Amy L, Quadros, Rolen M, Ribas, Antoni, Romberg, Neil, Roncarolo, Maria Grazia, Roth, Theodore L, Saco, Justin, Schickel, Jean-Nicolas, Schumann, Kathrin, Shifrut, Eric, Smith, Baz, Tobin, Victoria, Weissman, Jonathan S, Yu, Ruby   +44 more
core  

Chimeric piggyBac transposases for genomic targeting in human cells. [PDF]

, 2012
Integrating vectors such as viruses and transposons insert transgenes semi-randomly and can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location of insertion is required ...
Belcaid, Mahdi, Coates, Craig J, Dang, Nong C, Maragathavally, Kommineni J, Moisyadi, Stefan, Owens, Jesse B, Segal, David J, Stoytchev, Ilko, Stoytcheva, Zoia, Urschitz, Johann   +9 more
core   +5 more sources

Enhanced NK-92 Cytotoxicity by CRISPR Genome Engineering Using Cas9 Ribonucleoproteins

Frontiers in Immunology, 2020
Natural killer (NK) cells are an attractive cell-type for adoptive immunotherapy, but challenges in preparation of therapeutic primary NK cells restrict patient accessibility to NK cell immunotherapy. NK-92 is a well-characterized human NK cell line that
Rih-Sheng Huang, Hsin-An Shih, Min-Chi Lai, Yao-Jen Chang, Steven Lin   +4 more
semanticscholar   +1 more source

Therapeutic strategies for MMAE‐resistant bladder cancer through DPP4 inhibition

Molecular Oncology, EarlyView.
We established monomethyl auristatin E (MMAE)‐resistant bladder cancer (BC) cell lines by exposure to progressively increasing concentrations of MMAE in vitro. RNA sequencing showed DPP4 expression was increased in MMAE‐resistant BC cells. Both si‐DPP4 and the DPP4 inhibitor sitagliptin suppressed the viability of MMAE‐resistant BC cells.
Gang Li, Shuichi Tatarano, Hirofumi Yoshino, Saeki Saito, Mitsuhiko Tominaga, Junya Arima, Ikumi Fukuda, Takashi Sakaguchi, Ryosuke Matsushita, Yasutoshi Yamada, Hideki Enokida   +10 more
wiley   +1 more source

TP901-1 Phage Recombinase Facilitates Genome Engineering in Drosophila melanogaster

G3: Genes, Genomes, Genetics, 2019
Molecular biology techniques have a large impact on biomedical research and the availability of diverse tools to perform genome manipulations advances the ease of executing complicated genetic research.
Roumen Voutev, Richard S. Mann
doaj   +1 more source

Mapping the landscape of metabolic goals of a cell [PDF]

, 2016
Genome-scale flux balance models of metabolism provide testable predictions of all metabolic rates in an organism, by assuming that the cell is optimizing a metabolic goal known as the objective function.
Paschalidis, Ioannis Ch., Reznik, Ed, Segrè, Daniel, Stettner, Arion I., Zhao, Qi   +4 more
core   +3 more sources

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

Nucleic Acids Research, 2013
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems in bacteria and archaea use RNA-guided nuclease activity to provide adaptive immunity against invading foreign nucleic acids.
James E. DiCarlo, J. Norville, P. Mali, Xavier Rios, John D Aach, G. Church   +5 more
semanticscholar   +1 more source

Precision genome engineering in rice using prime editing system

Plant Biotechnology Journal, 2020
Making precise changes in the genomes of organisms is challenging for most genome editing tools. Recently, a search-and-replace method, also known as prime editing, was developed that can introduce user-defined sequence into a target site without ...
Kai Hua, Yuwei Jiang, Xiaoping Tao, Jian‐Kang Zhu   +3 more
semanticscholar   +1 more source