Results 181 to 190 of about 3,929,879 (390)

Mastering targeted genome engineering of GC-rich oleaginous yeast for tailored plant oil alternatives for the food and chemical sector [PDF]

, 2023
Pariya Shaigani, Tobias A. Fuchs, Petra Graban, Sophia Prem, Martina Haack, Mahmoud Masri, Norbert Mehlmer, Thomas Brueck   +7 more
openalex   +1 more source

RNA-Guided Human Genome Engineering via Cas9

Science, 2013
P. Mali, Luhan Yang, K. Esvelt, John D Aach, M. Guell, James E. DiCarlo, J. Norville, G. Church   +7 more
semanticscholar   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Genome engineering using the CRISPR-Cas9 system

Nature Protocols, 2013
F. Ran, P. Hsu, Jason B Wright, Vineeta Agarwala, Vineeta Agarwala, David A. Scott, Feng Zhang   +6 more
semanticscholar   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

, 2020
Kanut Laoharawee, Matthew J. Johnson, Walker S. Lahr, Joseph J. Peterson, Beau R. Webber, Branden S. Moriarity   +5 more
openalex   +2 more sources

A Highly Efficient CRISPR-Cas9-Based Genome Engineering Platform in Acinetobacter baumannii to Understand the H2O2-Sensing Mechanism of OxyR [PDF]

, 2019
Yu Wang, Zhipeng Wang, Yan Chen, Xiaoting Hua, Yunsong Yu, Quanjiang Ji   +5 more
openalex   +1 more source

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

Rapid, Scalable, and Combinatorial Genome Engineering by Markerless Enrichment and Recombination of Genetically Engineered Loci (MERGE) in Yeast

, 2022
Mudabir Abdullah, Brittany M. Greco, Jon M. Laurent, Riddhiman K. Garge, Daniel R. Boutz, Michelle Vandeloo, Edward M. Marcotte, Aashiq H. Kachroo   +7 more
openalex   +1 more source

A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii [PDF]

, 2022
Yoichiro Ito, Misa Ishigami, Goro Terai, Yasuyuki Nakamura, Noriko Hashiba, Teruyuki Nishi, Hikaru Nakazawa, Tomohisa Hasunuma, Kiyoshi Asai, Mitsuo Umetsu, Jun Ishii, Akihiko Kondo   +11 more
openalex   +1 more source