Results 111 to 120 of about 80,951 (303)

Discovery and Engineering of a Rat Endogenous Retrovirus Reverse Transcriptase for Efficient Prime Editing

Advanced Science, EarlyView.
We screened 558 reverse transcriptases and engineered an optimized rat endogenous retrovirus‐derived variant, enRERV‐RT, via structure‐guided engineering and deep mutational scanning. This enhanced prime editor, based on the engineered RT, outperforms conventional M‐MLV‐RT systems across plant and animal cells, particularly at hard‐to‐edit loci ...
Linsha Ma, Pengcheng Yao, Shengqi Wu, Yuanyuan Shi, Lang Qin, Baitao Li, Jiayi Zhu, Minhua Huang, Yichong Zhu, Yuwen Song, Jinhuan Pang, Ziping Guo, Guochuan Wu, Chen Wang, Kewei Xu, Ruihua Huang, Quan Kuang, Liang Qu, Changtian Pan, Xianrong Xie, Qinlong Zhu, Jiaying Huang, Qiupeng Lin   +22 more
wiley   +1 more source

High-Throughput Mining of Novel Compounds from Known Microbes: A Boost to Natural Product Screening

Molecules
Advanced techniques can accelerate the pace of natural product discovery from microbes, which has been lagging behind the drug discovery era. Therefore, the present review article discusses the various interdisciplinary and cutting-edge techniques to ...
Surya Nandan Meena, Anna Wajs-Bonikowska, Savita Girawale, Md Imran, Preethi Poduwal, Kisan M. Kodam   +5 more
doaj   +1 more source

Genomic Mining for Aspergillus Natural Products

Chemistry & Biology, 2006
The genus Aspergillus is renowned for its ability to produce a myriad of bioactive secondary metabolites. Although the propensity of biosynthetic genes to form contiguous clusters greatly facilitates assignment of putative secondary metabolite genes in the completed Aspergillus genomes, such analysis cannot predict gene expression and, ultimately ...
Bok, Jin Woo, Hoffmeister, Dirk, Maggio-Hall, Lori A., Murillo, Renato, Glasner, Jeremy D., Keller, Nancy P.   +5 more
openaire   +2 more sources

Genome Mining To Identify New Plant Triterpenoids [PDF]

, 2016
Arabidopsis thaliana is a well-established model organism for plant genetics, and its recently sequenced genome reveals a wealth of enzymes similar to known examples that biosynthesize secondary metabolites.
Gia C. Fazio (2717797), Seiichi P. T. Matsuda (1363896), Ran Xu (316855)   +2 more
core   +1 more source

Resistant Peanut Genotype Reprograms Rhizosphere Metabolism to Enhance Bacterial Wilt Suppression

Advanced Science, EarlyView.
The resistant peanut genotype selectively recruits beneficial bacteria, which coincides with the activation of salicylic acid (SA)‐dependent systemic acquired resistance (SAR) against Ralstonia solanacearum. Keystone rhizosphere metabolites are positively correlated with both beneficial microbiome assembly and SAR gene expression.
Rui Ren, Xiaogang Li, Hao Li, Zenghui Cao, Mengtian Hou, Chunlei Zhao, Nan Lou, Sasa Hu, Yanzhe Li, Qian Ma, Yaoyao Li, Yi Fan, Kunkun Zhao, Kai Zhao, Ding Qiu, Fangping Gong, Zhongfeng Li, Haitao Liu, Xingli Ma, Xingxiang Wang, Dongmei Yin   +20 more
wiley   +1 more source

A Conserved DT2‐bZIP66‐NF‐YC4 Regulatory Module Confers Drought Tolerance in Rice and Arabidopsis

Advanced Science, EarlyView.
This study identifies a conserved tripartiteDT2–bZIP66–NF‐YC4 transcriptional module that enhances rice drought tolerance by activating stress‐responsive genes. This regulatory complex is functionally conserved across cereals and Arabidopsis, providing promising targets for engineering drought‐resilient crops. ABSTRACT Drought stress severely restricts
Jun Shen, Yuchen Li, Liang Zhang, Xiaosong Ma, Chenyin Peng, Chunting Yuan, Yu Wu, Kaiyu Zhao, Sheng Zhao, Jiazhuo Guo, Run Li, Ziqian Yan, Shule Ren, Yige Han, Xin Lin, Xinyue Li, Ming Li, Pingfang Yang, Shiyong Song   +18 more
wiley   +1 more source

Genome Mining of Fungal Unique Trichodiene Synthase-like Sesquiterpene Synthases

Journal of Fungi
Sesquiterpenoids served as an important source for natural product drug discovery. Although genome mining approaches have revealed numerous novel sesquiterpenoids and biosynthetic enzymes, the comprehensive landscape of fungal sesquiterpene synthases ...
Zhanren Cong, Qiang Yin, Kunhong Tian, Njeru Joe Mukoma, Liming Ouyang, Tom Hsiang, Lixin Zhang, Lan Jiang, Xueting Liu   +8 more
doaj   +1 more source

Identification of a Nonribosomal Peptide Analog With Activity Against Multiple Gram‐Positive Bacteria via a Synthetic Bioinformatic Natural Product Discovery Approach

Advanced Science, EarlyView.
The genome of Rhodococcus erythropolis D‐1 was analyzed by bioinformatic tools to mine a novel nonribosomal peptide synthetase (NRPS) gene cluster. A nonribosomal peptide analog ZURJC5 associated with the NRPS was chemically synthesized. Through structure‐activity relationship studies, ZURJC28 was ultimately obtained and showed antibacterial activity ...
Keyi Chen, Jiayi Liang, Yujia Wu, Jianan Xu, Yi Liu, Wenguang Wang, Xinhang Jiang, Benjie Gao, Yueyue Wang, Hui Jiang   +9 more
wiley   +1 more source

The Metabolic Potential of the Human Lung Microbiome

Microorganisms
The human lung microbiome remains largely underexplored, despite its potential implications in the pharmacokinetics of inhaled drugs and its involvement in lung diseases.
Florian Semmler, Matheus Regis Belisário-Ferrari, Maria Kulosa, Leonard Kaysser   +3 more
doaj   +1 more source

5: Genome Mining [PDF]

, 2003
Genome sequencing projects generate a tremendous amount of data, which are stored in databanks and freely available through the Internet. Genome analysis has been dubbed genome mining in reference to the California Gold Rush in 1849.
Cold Spring Harbor Laboratory
core