Results 251 to 260 of about 1,823,070 (291)
Some of the next articles are maybe not open access.
2021
Next-generation sequencing (NGS) is a rapidly developing technology with a wide range of applications in biological sciences. Historically, DNA sequencing has evolved from the first to the second and third generation in less than 50 years. This chapter aims to provide guidance for designing NGS studies, performing NGS experiments and analysing NGS data,
openaire +2 more sources
Next-generation sequencing (NGS) is a rapidly developing technology with a wide range of applications in biological sciences. Historically, DNA sequencing has evolved from the first to the second and third generation in less than 50 years. This chapter aims to provide guidance for designing NGS studies, performing NGS experiments and analysing NGS data,
openaire +2 more sources
1988
The human genome contains 3 billion base pairs of DNA, sufficient to encode 100,000 to 300,000 genes. Since the number of genes that make up a human being is not known, this estimate is based upon a national average size for a mammalian gene. If the average gene is 30,000 bases long, there will be about 100,000 genes.
openaire +2 more sources
The human genome contains 3 billion base pairs of DNA, sufficient to encode 100,000 to 300,000 genes. Since the number of genes that make up a human being is not known, this estimate is based upon a national average size for a mammalian gene. If the average gene is 30,000 bases long, there will be about 100,000 genes.
openaire +2 more sources
Genome Sequencing and Genomics
2014This chapter explores issues related to Legionella genomics. A critical breakthrough in this area recently occurred with the determination of the genome sequences of three clinical, serogroup 1 isolates of Legionella pneumophila subsp. pneumophila. The three strains are L. pneumophila Paris, Lens and Philadelphia-1.
Carmen Buchrieser +3 more
openaire +1 more source
Interpreting Whole-Genome Sequencing
JAMA, 2014We believe that the report of the yield and interpretability of clinical whole-genome sequencing by Dr Dewey and colleagues1 is unduly pessimistic about the present and future efficacy of this molecular genetic technology in clinical medicine. Their experience of low coverage of key disease genes, poor nucleotide-calling reproducibility, low diagnostic
Wayne W, Grody +2 more
openaire +2 more sources
Genome sequencing: Illuminating the sunflower genome
Nature Plants, 2017A high-quality sunflower genome provides insight into Asterid genome evolution. Moreover, integrative analyses based on quantitative genetics, expression and diversity data uncover the gene networks and candidate genes for oil metabolism and flowering time, two important agronomic traits for sunflowers.
openaire +2 more sources
Toward nanoscale genome sequencing
Trends in Biotechnology, 2007This article reports on the state-of-the-art technologies that sequence DNA using miniaturized devices. The article considers the miniaturization of existing technologies for sequencing DNA and the opportunities for cost reduction that 'on-chip' devices can deliver.
Declan, Ryan +4 more
openaire +2 more sources
2014
The current Lotus japonicus reference genome sequence is based on a hybrid assembly of Sanger TAC/BAC, Sanger shotgun and Illumina shotgun sequencing data generated from the Miyakojima-MG20 accession. It covers nearly all expressed L. japonicus genes and has been annotated mainly based on transcriptional evidence.
Sato, Shusei, Andersen, Stig Uggerhøj
openaire +1 more source
The current Lotus japonicus reference genome sequence is based on a hybrid assembly of Sanger TAC/BAC, Sanger shotgun and Illumina shotgun sequencing data generated from the Miyakojima-MG20 accession. It covers nearly all expressed L. japonicus genes and has been annotated mainly based on transcriptional evidence.
Sato, Shusei, Andersen, Stig Uggerhøj
openaire +1 more source
Science, 1997
The human genome project is at the halfway point. The genomes of 11 microbes, Escherichia coli , and yeast are finished, yet the human genome is only 2 percent finished. The scale-up to finish by 2005 presents a significant challenge.
L, Rowen, G, Mahairas, L, Hood
openaire +2 more sources
The human genome project is at the halfway point. The genomes of 11 microbes, Escherichia coli , and yeast are finished, yet the human genome is only 2 percent finished. The scale-up to finish by 2005 presents a significant challenge.
L, Rowen, G, Mahairas, L, Hood
openaire +2 more sources
Genome Sequencing and Assembly
2011Decoding the genome sequence is becoming a fundamental tool for molecular, genetic, and genomic studies. This chapter reviews the history of DNA sequencing and technical principles of different sequencing platforms, and compares the strengths and weaknesses of different techniques for high-throughput genome sequencing applications are compared. It also
Manfred G, Grabherr +2 more
openaire +2 more sources