Results 21 to 30 of about 1,556,351 (302)
Microbiome, 2017 Background Colonization by the pathogen Clostridium difficile often occurs in the background of a disrupted microbial community. Identifying specific organisms conferring resistance to invasion by C.
Sepideh Pakpour +7 more
doaj +1 more source
Four chromosome scale genomes and a pan-genome annotation to accelerate pecan tree breeding
Nature Communications, 2021 Pecan is an important specialty crop that has experienced extensive interspecific hybridization and nearly-obligate outcrossing. Here, the authors assemble diploid genomes of four outbred genotypes, identify interspecific introgressions through ...
John T. Lovell +34 more
doaj +1 more source
Hidden biases in germline structural variant detection
Genome Biology, 2021 Background Genomic structural variations (SV) are important determinants of genotypic and phenotypic changes in many organisms. However, the detection of SV from next-generation sequencing data remains challenging.
Michael M. Khayat +10 more
doaj +1 more source
Nature, 2000 AbstractComplete genome sequences of 30 microbial species have been determined during the past five years, and work in progress indicates that the complete sequences of more than 100 further microbial species will be available in the next two to four years.
Fraser, Claire M. +2 more
openaire +2 more sources
Detecting mutations in mixed sample sequencing data using empirical Bayes [PDF]
, 2012 We develop statistically based methods to detect single nucleotide DNA mutations in next generation sequencing data. Sequencing generates counts of the number of times each base was observed at hundreds of thousands to billions of genome positions in ...
Bell, John +4 more
core +3 more sources
Whole-genome sequencing of Klebsiella pneumoniae isolates to track strain progression in a single patient with recurrent urinary tract infection [PDF]
, 2019 Klebsiella pneumoniae is an important uropathogen that increasingly harbors broad-spectrum antibiotic resistance determinants. Evidence suggests that some same-strain recurrences in women with frequent urinary tract infections (UTIs) may emanate from a ...
Minx, Patrick J +3 more
core +5 more sources
Pre-sequencing assessment of RNA-Seq library quality using real-time qPCR
BioTechniquesRNA sequencing (RNA-Seq) is an essential assay for studying transcriptome profiling. Ribosomal RNA (rRNA) comprises more than 80–90% of total cellular RNA; efficient removal is essential for accurately capturing transcriptomes, particularly to sequence ...
Kavya Kottapalli +13 more
doaj +1 more source
Complete genome sequences of three African foot-and-mouth disease viruses from clinical samples isolated in 2009 and 2010 [PDF]
, 2016 <p>The complete genome sequences of three foot-and-mouth disease viruses (one virus of each serotype SAT1, SAT2 and O) were directly sequenced from RNA extracted from clinical bovine samples, demonstrating the feasibility of full-genome sequencing ...
De Clercq, Kris +8 more
core +1 more source
Proceedings of the National Academy of Sciences, 1984 Unique DNA sequences can be determined directly from mouse genomic DNA. A denaturing gel separates by size mixtures of unlabeled DNA fragments from complete restriction and partial chemical cleavages of the entire genome. These lanes of DNA are transferred and UV-crosslinked to nylon membranes.
Church, George, Gilbert, Walter
openaire +3 more sources
BMC Biology, 2017 Background The de novo assembly of repeat-rich mammalian genomes using only high-throughput short read sequencing data typically results in highly fragmented genome assemblies that limit downstream applications.
Peter A. Larsen +19 more
doaj +1 more source