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Assembling Plant Genomes with Long-Read Sequencing
2022Continuous improvements in long-read sequencing allow us to tackle increasingly big and complex genomes. Here we present the principles of long-read genome assembly, taking Solanum pennellii nanopore sequencing as an example.
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Genome Sequencing, Assembly, and Annotation
2019Strategies and the Next Generation Technologies applied for the sequencing and assembly of genomes are discussed in this chapter. Recent methodologies adapted for sequencing the fungal, plant, animal, degraded and ancient fossil genomes are provided with appropriate examples.
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Allele Identification in Assembled Genomic Sequence Datasets
2012Allelic variation within species provides fundamental insights into the evolution and ecology of organisms, and information about this variation is becoming increasingly available in sequence datasets of multiple and/or outbred individuals. Unfortunately, identifying true allelic variants poses a number of challenges, given the presence of both ...
Katrina M, Dlugosch, Aurélie, Bonin
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Sequencing and Assembling Genomes and Chromosomes of Cereal Crops
2019Next generation sequencing (NGS) and assembly are key pieces in the success of cereal crops genomes sequencing. One interesting strategy for reducing the complexity in large genomes, the case of several cereal crops, is the sequencing of individual chromosomes. This has been done with success by flow cytometric chromosome sorting followed by sequencing
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Sequencing, Assembling, and Finishing Complete Bacteriophage Genomes
2017Next-generation DNA sequencing (NGS) technologies have made generating genomic sequence for organisms of interest affordable and commonplace. However, NGS platforms and analysis software are generally tuned to be used on large and complex genomes or metagenomic samples.
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Complex genome assembly based on long-read sequencing
Briefings in Bioinformatics, 2022Guohua Wang, Tianjiao Zhang, Yr Jia
exaly
Benchmarking genome assembly methods on metagenomic sequencing data
Briefings in Bioinformatics, 2023Xiaodong Fang +2 more
exaly
The genome of Lithobates catesbeianus Genome sequencing and assembly
A chromosome-level reference genome for American ...openaire +1 more source
Benchmarking multi-platform sequencing technologies for human genome assembly
Briefings in Bioinformatics, 2023Huang Yufen +2 more
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