Results 51 to 60 of about 421,782 (313)

Cross-species optimization of nuclei isolation in ten plant species

Plant Methods
Single-cell technologies are transforming plant biology, yet broadly transferable nuclei isolation remains a key bottleneck for snRNA-seq. We developed a reproducible, cost-efficient Percoll-based workflow that is applicable to multiple maize tissues and
Yun Luo, Jiali Yan, Thuy La, Edward S. Buckler, Jianbing Yan, M. Cinta Romay   +5 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Extensive genomic diversity and selective conservation of virulence determinants in enterohemorrhagic Escherichia coli strains of O157 and non O157 serotypes [PDF]

, 2007
Background: Enterohemorrhagic Escherichia coli (EHEC) O157 causes severe food-borne illness in humans. The chromosome of O157 consists of 4.1 Mb backbone sequences shared by benign E.
Nougayrède, Jean-Philippe, Tashiro, Kousuke, Ogura, Y, Nakayama, K, Terajima, Jun, Oswald, Eric, Ogura, Yoshitoshi, Tobe, T, Hayashi, T, Ooka, T, Asadulghani, -, Kuhara, Satoru, Watanabe, Haruo, Kurokawa, Ken, Tashiro, K, Watanabe, H, Asadulghani, XX, Ooka, Tadasuke, Kurokawa, K, Nakayama, Keisuke, Hayashi, Tetsuya, Kuhara, S, Tobe, Toru, Terajima, J   +23 more
core   +1 more source

Increased experimental conditions and marker densities identified more genetic loci associated with southern and northern leaf blight resistance in maize

Scientific Reports, 2018
Southern leaf blight (SLB) and northern leaf blight (NLB) are the two major foliar diseases limiting maize production worldwide. Upon previous study with the nested association mapping (NAM) population, which consist of 5,000 recombinant inbred lines ...
Yong-xiang Li, Lin Chen, Chunhui Li, Peter J. Bradbury, Yun-su Shi, Yanchun Song, Dengfeng Zhang, Zhiwu Zhang, Edward S. Buckler, Yu Li, Tianyu Wang   +10 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

High frequency of antimicrobial resistance in Salmonella and Escherichia coli causing diarrheal diseases at the Yirimadio community health facility, Mali

BMC Microbiology
Background Diarrhoea is a public health problem, especially in developing countries where it is the second leading cause of child mortality. In Low Income Countries like in Mali, self-medication and inappropriate use of antibiotics due to the scarcity of
Bintou Diarra, Ibréhima Guindo, Boī Koné, Maīmouna Dembélé, Ibrahim Cissé, Souleymane Thiam, Kadidia Konaté, Mamadou Tékété, Almoustapha Maīga, Oumou Maīga, Lassina Timbiné, Abdoulaye Djimde   +11 more
doaj   +1 more source

Development and application of Single Primer Enrichment Technology (SPET) SNP assay for population genomics analysis and candidate gene discovery in lettuce

Frontiers in Plant Science, 2023
Single primer enrichment technology (SPET) is a novel high-throughput genotyping method based on short-read sequencing of specific genomic regions harboring polymorphisms.
Pasquale Tripodi, Massimiliano Beretta, Damien Peltier, Ilias Kalfas, Christos Vasilikiotis, Anthony Laidet, Gael Briand, Charlotte Aichholz, Tizian Zollinger, Rob van Treuren, Davide Scaglione, Sandra Goritschnig   +11 more
doaj   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

Burkholderia pseudomallei genome plasticity associated with genomic island variation

, 2008
SJP was supported by a Wellcome Trust Career Development Award in Clinical Tropical Medicine. This study was funded by the Wellcome Trust.Background: Burkholderia pseudomallei is a soil-dwelling saprophyte and the cause of melioidosis.
Direk Limmathurotsakul, William C Nierman, Mongkol Vesaratchavest, Vesaratchavest, Mongkol, Nierman William C, Nierman, WC, Limmathurotsakul Direk, Peacock Sharon J, Chierakul, Wirongrong, Chierakul Wirongrong, Nicholas PJ Day, Nierman, William C., Day Nicholas PJ, Vesaratchavest, M, Holden, Matthew T. G., Wirongrong Chierakul, Feil, Edward J., Peacock, Sharon J., Peacock, SJ, Currie, B J, Wuthiekanun, V, Tumapa Sarinna, Matthew TG Holden, Holden, M T G, Holden Matthew TG, Currie, BJ, Tumapa, S, Feil Edward J, Feil, EJ, Wuthiekanun Vanaporn, Sharon J Peacock, Day, Nicholas P. J., Peacock, S J, Day, NP, Nierman, W C, Holden, MT, Currie Bart J, Day, N P J, Limmathurotsakul, Direk, Limmathurotsakul, D, Tumapa, Sarinna, Wuthiekanun, Vanaporn, Edward J Feil, Vesaratchavest Mongkol, Currie, Bart J., Sarinna Tumapa, Vanaporn Wuthiekanun, Chierakul, W, Day, Nicholas, Feil, E J, Bart J Currie   +50 more
core   +1 more source

Understanding the functionality of transcript diversity [PDF]

, 2007
Recent years have seen a huge increase in the amount of genomic DNA being sequenced from a wide variety of organisms, giving us an unprecedented insight into the molecular diversity seen in nature.
Harrington, Eoghan
core   +1 more source