Results 61 to 70 of about 714,798 (302)

Cross-species optimization of nuclei isolation in ten plant species

Plant Methods
Single-cell technologies are transforming plant biology, yet broadly transferable nuclei isolation remains a key bottleneck for snRNA-seq. We developed a reproducible, cost-efficient Percoll-based workflow that is applicable to multiple maize tissues and
Yun Luo, Jiali Yan, Thuy La, Edward S. Buckler, Jianbing Yan, M. Cinta Romay   +5 more
doaj   +1 more source

Diversity of immunoglobulin light chain genes in non-teleost ray-finned fish uncovers IgL subdivision into five ancient isotypes [PDF]

, 2018
The aim of this study was to fill important gaps in the evolutionary history of immunoglobulins by examining the structure and diversity of IgL genes in non-teleost ray-finned fish.
Andreyushkova, Daria A, Baranov, Konstantin O, Chikaev, Nikolai A, Gessner, Jörn, Guselnikov, Sergey V, Kulemzin, Sergey V, Makunin, Alexey I, Mechetina, Ludmila V, Najakshin, Alexander M, Schartl, Manfred, Stöck, Matthias, Taranin, Alexander V, Trifonov, Vladimir A, Warren, Wesley C, Wuertz, Sven   +14 more
core   +5 more sources

Bacterial riboproteogenomics : the era of N-terminal proteoform existence revealed [PDF]

, 2020
With the rapid increase in the number of sequenced prokaryotic genomes, relying on automated gene annotation became a necessity. Multiple lines of evidence, however, suggest that current bacterial genome annotations may contain inconsistencies and are ...
Fijalkowska, Daria, Fijalkowski, Igor, Van Damme, Petra, Willems, Patrick   +3 more
core   +2 more sources

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

Increased experimental conditions and marker densities identified more genetic loci associated with southern and northern leaf blight resistance in maize

Scientific Reports, 2018
Southern leaf blight (SLB) and northern leaf blight (NLB) are the two major foliar diseases limiting maize production worldwide. Upon previous study with the nested association mapping (NAM) population, which consist of 5,000 recombinant inbred lines ...
Yong-xiang Li, Lin Chen, Chunhui Li, Peter J. Bradbury, Yun-su Shi, Yanchun Song, Dengfeng Zhang, Zhiwu Zhang, Edward S. Buckler, Yu Li, Tianyu Wang   +10 more
doaj   +1 more source

Transcriptional Regulation: a Genomic Overview [PDF]

, 2002
The availability of the Arabidopsis thaliana genome sequence allows a comprehensive analysis of transcriptional regulation in plants using novel genomic approaches and methodologies. Such a genomic view of transcription first necessitates the compilation
Riechmann, José Luis
core   +2 more sources

Pseudomonas aeruginosa Genomic Structure and Diversity [PDF]

Frontiers in Microbiology, 2011
The Pseudomonas aeruginosa genome (G + C content 65-67%, size 5.5-7 Mbp) is made up of a single circular chromosome and a variable number of plasmids. Sequencing of complete genomes or blocks of the accessory genome has revealed that the genome encodes a large repertoire of transporters, transcriptional regulators, and two-component regulatory systems ...
Klockgether, Jens, Cramer, Nina, Wiehlmann, Lutz, Davenport, Colin F., Tümmler, Burkhard   +4 more
openaire   +3 more sources

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

High frequency of antimicrobial resistance in Salmonella and Escherichia coli causing diarrheal diseases at the Yirimadio community health facility, Mali

BMC Microbiology
Background Diarrhoea is a public health problem, especially in developing countries where it is the second leading cause of child mortality. In Low Income Countries like in Mali, self-medication and inappropriate use of antibiotics due to the scarcity of
Bintou Diarra, Ibréhima Guindo, Boī Koné, Maīmouna Dembélé, Ibrahim Cissé, Souleymane Thiam, Kadidia Konaté, Mamadou Tékété, Almoustapha Maīga, Oumou Maīga, Lassina Timbiné, Abdoulaye Djimde   +11 more
doaj   +1 more source

Development and application of Single Primer Enrichment Technology (SPET) SNP assay for population genomics analysis and candidate gene discovery in lettuce

Frontiers in Plant Science, 2023
Single primer enrichment technology (SPET) is a novel high-throughput genotyping method based on short-read sequencing of specific genomic regions harboring polymorphisms.
Pasquale Tripodi, Massimiliano Beretta, Damien Peltier, Ilias Kalfas, Christos Vasilikiotis, Anthony Laidet, Gael Briand, Charlotte Aichholz, Tizian Zollinger, Rob van Treuren, Davide Scaglione, Sandra Goritschnig   +11 more
doaj   +1 more source