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Characterization of Sigma Factor Genes in Streptomyces lividans TK24 Using a Genomic Library-Based Approach for Multiple Gene Deletions [PDF]
Frontiers in Microbiology, 2018 Alternative sigma factors control numerous aspects of bacterial life, including adaptation to physiological stresses, morphological development, persistence states and virulence.
Yuriy Rebets +23 more
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High-Efficiency Transformation and Expression of Genomic Libraries in Yeast [PDF]
Methods and Protocols, 2023 Saccharomyces cerevisiae is a powerful system for the expression of genome-wide or combinatorial libraries for diverse types of screening. However, expressing large libraries in yeast requires high-efficiency transformation and controlled expression ...
Mira Loock +5 more
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Construction of a Cosmid-Based Ultraefficient Genomic Library System for Filamentous Fungi of the Genus Aspergillus [PDF]
Journal of FungiFilamentous fungi of the genus Aspergillus include producers of industrially important organic acids, enzymes, and secondary metabolites, as well as pathogens of many plants and animals.
Chihiro Kadooka, Takuji Oka
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Preparation of Genomic Overexpression Library
Bio-Protocol, 2012 This protocol is used to identify/select for Escherichia coli genes that, when overexpressed in Vibrio cholerae Hsp33 (hslO gene) deletion mutant, protect against oxidative heat stress and, by extension, against HOCl-mediated protein damage.
Wei-Yun Wholey
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Metagenomics and Whole Genome Sequencing in Clinical Microbiology: A Narrative Review [PDF]
Journal of Clinical and Diagnostic Research, 2023 The role of Whole Genome Sequencing (WGS) in clinical microbiology is increasing and is not restricted to molecular epidemiology alone. With the advent of third-generation sequencing technologies, its applications in infectious disease diagnostics have ...
Preeti Thakur, Indira Verma
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ABCS Health Sciences, 2023 Introduction: Species A rotavirus (RVA) infections are a major cause of severe gastroenteritis in children of
Gerlane dos Santos Barros +3 more
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Czech Journal of Genetics and Plant Breeding, 2023 Simple sequence repeat (SSR) markers are valuable genetic and genomic research tools and are extensively used in major crops. However, a paucity of available molecular markers in lentils (Lens culinaris Medik.) has significantly hindered genomic studies ...
Melike Bakir +3 more
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Viruses, 2022 The development of antibody therapies against SARS-CoV-2 remains a challenging task during the ongoing COVID-19 pandemic. All approved therapeutic antibodies are directed against the receptor binding domain (RBD) of the spike, and therefore lose ...
Rico Ballmann +14 more
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Phage-DMS: A Comprehensive Method for Fine Mapping of Antibody Epitopes
iScience, 2020 Summary: Understanding the antibody response is critical to developing vaccine and antibody-based therapies and has inspired the recent development of new methods to isolate antibodies.
Meghan E. Garrett +5 more
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Libraries for genomic SELEX [PDF]
Nucleic Acids Research, 1997 An increasing number of proteins are being identified that regulate gene expression by binding specific nucleic acidsin vivo. A method termed genomic SELEX facilitates the rapid identification of networks of protein-nucleic acid interactions by identifying within the genomic sequences of an organism the highest affinity sites for any protein of the ...
B S, Singer +3 more
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