Large Insert Environmental Genomic Library Production [PDF]
Journal of Visualized Experiments, 2009 The vast majority of microbes in nature currently remain inaccessible to traditional cultivation methods. Over the past decade, culture-independent environmental genomic (i.e. metagenomic) approaches have emerged, enabling researchers to bridge this cultivation gap by capturing the genetic content of indigenous microbial communities directly from the ...
Steven J. Hallam +4 more
semanticscholar +5 more sources
Construction and Application of a Baculovirus Genomic Library [PDF]
Zeitschrift für Naturforschung C, 2009 A random genomic library of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) was constructed and viral factors were identified by screening the regulator(s) for helicase gene expression. DNAs of 238 library plasmids were used to co-transfect with the reporter plasmid, pHp510-luc, in which the luciferase (luc) gene was driven by the baculovirus ...
Xu'ai Lin +4 more
semanticscholar +5 more sources
Characterization of Sigma Factor Genes in Streptomyces lividans TK24 Using a Genomic Library-Based Approach for Multiple Gene Deletions. [PDF]
Front Microbiol, 2018 Alternative sigma factors control numerous aspects of bacterial life, including adaptation to physiological stresses, morphological development, persistence states and virulence.
Rebets Y +19 more
europepmc +4 more sources
Construction of a genomic library of the food spoilage yeastZygosaccharomyces bailiiand isolation of the β-isopropylmalate dehydrogenase gene (ZbLEU2) [PDF]
FEMS Yeast Research, 2001 A genomic library of the yeast Zygosaccharomyces bailii ISA 1307 was constructed in pRS316, a shuttle vector for Saccharomyces cerevisiae and Escherichia coli.
Fernando Rodrigues +6 more
openalex +2 more sources
Isolation and chromosomal localization of unique DNA sequences from a human genomic library.
Proceedings of the National Academy of Sciences of the United States of America, 1982 Recombinant bacteriophage lambda from a human genomic library were screened to indentify human DNA inserts having only unique sequences. Unique human inserts were found in about 1% of the phage screened. One recombinant phage, P3-2, was studied in detail.
Fa‐Ten Kao +3 more
openalex +2 more sources
A Fast and Efficient Single-stranded Genomic Library Preparation Method Optimized for Ancient DNA. [PDF]
J Hered, 2021 We present a protocol to prepare extracted DNA for sequencing on the Illumina sequencing platform that has been optimized for ancient and degraded DNA. Our approach, the Santa Cruz Reaction or SCR, uses directional splinted ligation of Illumina’s P5 and ...
Kapp JD, Green RE, Shapiro B.
europepmc +2 more sources
Using partial genomic fosmid libraries for sequencing complete organellar genomes [PDF]
BioTechniques, 2006 Organellar genome sequences provide numerous phylogenetic markers and yield insight into organellar function and molecular evolution. These genomes are much smaller in size than their nuclear counterparts; thus, their complete sequencing is much less expensive than total nuclear genome sequencing, making broader phylogenetic sampling feasible. However;
Jeffrey L. Boore +6 more
openaire +5 more sources
A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits. [PDF]
BMC Plant Biol, 2016 BackgroundGenomic libraries of introgression lines (ILs) consist of collections of homozygous lines with a single chromosomal introgression from a donor genotype in a common, usually elite, genetic background, representing the whole donor genome in the ...
Perpiñá G +4 more
europepmc +2 more sources
Construction of a Cosmid-Based Ultraefficient Genomic Library System for Filamentous Fungi of the Genus Aspergillus. [PDF]
J Fungi (Basel)Filamentous fungi of the genus Aspergillus include producers of industrially important organic acids, enzymes, and secondary metabolites, as well as pathogens of many plants and animals.
Kadooka C, Oka T.
europepmc +2 more sources
Metagenomics and Whole Genome Sequencing in Clinical Microbiology: A Narrative Review [PDF]
Journal of Clinical and Diagnostic Research, 2023 The role of Whole Genome Sequencing (WGS) in clinical microbiology is increasing and is not restricted to molecular epidemiology alone. With the advent of third-generation sequencing technologies, its applications in infectious disease diagnostics have ...
Preeti Thakur, Indira Verma
doaj +1 more source