Results 31 to 40 of about 633,728 (345)
Test: Construction of genomic libraries [PDF]
Biochemistry and Molecular Biology Education, 2005 AbstractTerms to be familiar with before you start to solve the test: genomic library, gel filtration, restriction endonuclease, plasmid, sticky ends, blunt ends, ligation, recombinant DNA, bacterium transformation, denaturation and renaturation of DNA, satellite DNA, telomere, centromere, unique and repetitive sequences.
openaire +2 more sources
Chromosome-segment scanning for gain- or loss-of-function screening (CHASING)
iScienceSummary: Identifying beneficial or functional mutations for a specific species is a task always relies on the labor-intensive construction of a library containing thousands of single-gene knockout or interference strains.
Yan Xia +7 more
doaj +1 more source
Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries [PDF]
Scientific Reports, 2017 AbstractCRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed applications. Here we present the first individually cloned CRISPR-Cas9 genome wide arrayed sgRNA libraries covering 17,166 ...
Metzakopian, Emmanouil +14 more
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Exploring genome wide bisulfite sequencing for DNA methylation analysis in livestock: a technical assessment [PDF]
, 2014 peer-reviewedRecent advances made in “omics” technologies are contributing to a revolution in livestock selection and breeding practices. Epigenetic mechanisms, including DNA methylation are important determinants for the control of gene expression in ...
Couldrey, Christine, Doherty, Rachael
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Preliminary Study of BAC Library Construction in Black Tiger Shrimp, Penaeus monodon
Walailak Journal of Science and Technology, 2011 Availability of shrimp genome information is necessary for shrimp genetic studies and large-insert DNA clones, bacterial artificial chromosome (BACs) serve as valuable tools for obtaining genomic sequences.
Suwit WUTHISUTHIMETHAVEE +3 more
doaj +1 more source
Microsatellite primers for red drum (Sciaenops ocellatus) [PDF]
, 2008 In this note, we document polymerase-chain-reaction (PCR) primer pairs for 101 nuclear-encoded microsatellites designed and developed from a genomic library for red drum (Sciaenops ocellatus).
Gold, John R. +3 more
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Identification and validation of microsatellite markers in strawberry tree (Arbutusunedo L.) [PDF]
, 2019 Strawberry tree (Arbutus unedo L.), an evergreen shrub/small tree of the family Ericaceae, is a main constituent of the Mediterranean basin flora; although it is also found in southwestern Prance, Macaronesia, and Ireland. The small fruits are edible but
Carlier, Jorge +4 more
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Function‐driven design of a surrogate interleukin‐2 receptor ligand
FEBS Letters, EarlyView.Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang +9 more
wiley +1 more source
Genomic resource development for a diploid mint: Mentha longifolia [PDF]
, 2010 This research project aimed to develop genomic resources needed to enable construction of a genetic linkage map of the diploid mint species Mentha longifolia.
Hadadian, Zahra
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Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions
FEBS Letters, EarlyView.Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf +6 more
wiley +1 more source