Results 51 to 60 of about 2,587,498 (385)
Scientific Reports, 2022 The full-length double-strand cDNA sequencing, one of the RNA-Seq methods, is a powerful method used to investigate the transcriptome status of a gene of interest, such as its transcription level and alternative splicing variants.
Hiroki Ura, Sumihito Togi, Yo Niida
doaj +1 more source
Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries [PDF]
Scientific Reports, 2017 AbstractCRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed applications. Here we present the first individually cloned CRISPR-Cas9 genome wide arrayed sgRNA libraries covering 17,166 ...
Metzakopian, Emmanouil +14 more
openaire +3 more sources
PANTHER: a library of protein families and subfamilies indexed by function.
Genome Research, 2003 In the genomic era, one of the fundamental goals is to characterize the function of proteins on a large scale. We describe a method, PANTHER, for relating protein sequence relationships to function relationships in a robust and accurate way.
P. Thomas +8 more
semanticscholar +1 more source
10x Genomics Library Construction v1 [PDF]
, 2020 10x Genomics Library Construction
Graham J Etherington +9 more
openaire +1 more source
Fast genomic optical map assembly algorithm using binary representation [PDF]
arXiv, 2022 Reducing the cost of sequencing genomes provided by next-generation sequencing technologies has greatly increased the number of genomic projects. As a result, there is a growing need for better assembly and assembly validation methods. One promising idea is to use heterogeneous data in assembly projects. Optical Mapping (OM) is beneficial in validating
arxiv
The Parallelism Motifs of Genomic Data Analysis [PDF]
, 2020 Genomic data sets are growing dramatically as the cost of sequencing continues to decline and small sequencing devices become available. Enormous community databases store and share this data with the research community, but some of these genomic data analysis problems require large scale computational platforms to meet both the memory and ...
arxiv +1 more source
GIVE: portable genome browsers for personal websites. [PDF]
, 2018 Growing popularity and diversity of genomic data demand portable and versatile genome browsers. Here, we present an open source programming library called GIVE that facilitates the creation of personalized genome browsers without requiring a system ...
Alvin Zheng +31 more
core +2 more sources
Physiological Genomics, 2010 The limited availability of genomic tools and data for nonmodel species impedes computational and systems biology approaches in nonmodel organisms. Here we describe the development, functional annotation, and utilization of genomic tools for the avian ...
Arun Rawat +8 more
semanticscholar +1 more source
Collection of prokaryotic genome contents expectation rules from scientific literature [PDF]
arXiv, 2023 Shaped by natural selection and other evolutionary forces, an organism's evolutionary history is reflected through its genome sequence, content of functional elements and organization. Consequently, organisms connected through phylogeny, metabolic or morphological traits, geographical proximity, or habitat features are likely to exhibit similarities in
arxiv
Inexpensive Multiplexed Library Preparation for Megabase-Sized Genomes
bioRxiv, 2015 Whole-genome sequencing has become an indispensible tool of modern biology. However, the cost of sample preparation relative to the cost of sequencing remains high, especially for small genomes where the former is dominant. Here we present a protocol for
M. Baym +5 more
semanticscholar +1 more source