Results 61 to 70 of about 633,728 (345)

Feasibility of a ctDNA multigenic panel for non‐small‐cell lung cancer early detection and disease surveillance

Molecular Oncology, EarlyView.
Plasma‐based detection of actionable mutations is a promising approach in lung cancer management. Analysis of ctDNA with a multigene NGS panel identified TP53, KRAS, and EGFR as the most frequently altered, with TP53 and KRAS in treatment‐naïve patients and TP53 and EGFR in previously treated patients.
Giovanna Maria Stanfoca Casagrande, Marcela de Oliveira Silva, Mariana Bisarro dos Reis, Rodrigo de Oliveira Cavagna, Luciane Sussuchi, Icaro Alves Pinto, Natalia Zampieri Pontes, Rodrigo Sampaio Chiarantano, Flavio Augusto Ferreira da Silva, Pedro de Marchi, Letícia Ferro Leal, Rui M. Reis   +11 more
wiley   +1 more source

Computational analysis of genes with lethal knockout phenotype and prediction of essential genes in archaea

mBio
The identification of microbial genes essential for survival as those with lethal knockout phenotype (LKP) is a common strategy for functional interrogation of genomes.
Kira S. Makarova, Changyi Zhang, Yuri I. Wolf, Svetlana Karamycheva, Rachel J. Whitaker, Eugene V. Koonin   +5 more
doaj   +1 more source

Generation of High-Value Genomic Resource in Rice: A “Subgenomic Library” of Low-Light Tolerant Rice Cultivar Swarnaprabha

Biology, 2023
Rice is the major staple food crop for more than 50% of the world’s total population, and its production is of immense importance for global food security. As a photophilic plant, its yield is governed by the quality and duration of light.
Sovanlal Sahu, Payal Gupta, Thirumalanahalli Prakash Gowtham, Kumar Shiva Yogesh, Tenkabailu Dharmanna Sanjay, Ayushi Singh, Hay Van Duong, Sharat Kumar Pradhan, Deepak Singh Bisht, Nagendra Kumar Singh, Mirza J. Baig, Rhitu Rai, Prasanta K. Dash   +12 more
doaj   +1 more source

Tumor and germline testing with next generation sequencing in epithelial ovarian cancer: a prospective paired comparison using an 18‐gene panel

Molecular Oncology, EarlyView.
Genetic testing in epithelial ovarian cancer includes both germline and tumor‐testing. This approach often duplicates resources. The current prospective study assessed the feasibility of tumor‐first multigene testing by comparing tumor tissue with germline testing of peripheral blood using an 18‐gene NGS panel in 106 patients.
Elisabeth Spenard, Cristina Mitric, Melanie Care, Tracy L. Stockley, Raymond H. Kim, Jeanna McCuaig, Blaise Clarke, Laura Ranich, Clare Sheen, Sarah E. Ferguson, Liat Hogen, Taymaa May, Marcus Q. Bernardini   +12 more
wiley   +1 more source

Genome-Scale Validation of Deep-Sequencing Libraries

PLoS ONE, 2008
Chromatin immunoprecipitation followed by high-throughput (HTP) sequencing (ChIP-seq) is a powerful tool to establish protein-DNA interactions genome-wide. The primary limitation of its broad application at present is the often-limited access to sequencers.
D. Schmidt†, R. Stark†, M. D. Wilson, G. D. Brown, D. T. Odom.   +4 more
openaire   +4 more sources

AnnotationSketch: a genome annotation drawing library [PDF]

Bioinformatics, 2008
Abstract Summary: To analyse the vast amount of genome annotation data available today, a visual representation of genomic features in a given sequence range is required. We developed a C library which provides layout and drawing capabilities for annotation features.
Sascha, Steinbiss, Gordon, Gremme, Christin, Schärfer, Malte, Mader, Stefan, Kurtz   +4 more
openaire   +2 more sources

STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods [PDF]

, 2017
The growing interest in liquid biopsies for cancer research and cell-based non-invasive prenatal testing (NIPT) invigorates the need for improved single cell analysis.
Cornelis, Senne, Deforce, Dieter, Deleye, Lieselot, Tilleman, Laurentijn, Van Nieuwerburgh, Filip, Vander Plaetsen, Ann-Sophie   +5 more
core   +2 more sources

Plasma extrachromosomal circular DNA as a biomarker in EGFR‐targeted therapy of non‐small cell lung cancer

Molecular Oncology, EarlyView.
Detection of extrachromosomal circular DNA (eccDNA) in plasma samples from EGFR‐mutated non‐small cell lung cancer patients. Plasma was collected before and during treatment with the EGFR‐tyrosine kinase inhibitor osimertinib. Plasma eccDNA was detected in all cancer samples, and the presence of the EGFR gene on eccDNA serves as a potential biomarker ...
Simone Stensgaard, Sarmad Mehmood, Eva Boysen Fynboe Ebert, Peter Meldgaard, Anindya Dutta, Boe S. Sorensen   +5 more
wiley   +1 more source

A PCR-based method for isolation of genomic DNA flanking a known DNA sequence [PDF]

, 1999
We describe a simple PCR-based method for the isolation of genomic DNA that lies adjacent to a known DNA sequence. The method is based on the directional cloning of digested genomic DNA into the multiple cloning site of a pUC-based plasmid to generate a ...
Boulter, CA, Natarajan, D
core  

Genome sequence of the Lebeckia ambigua-nodulating 'Burkholderia sprentiae' strain WSM5005T [PDF]

, 2013
"Burkholderia sprentiae" strain WSM5005(T) is an aerobic, motile, Gram-negative, non-sporeforming rod that was isolated in Australia from an effective N-2-fixing root nodule of Lebeckia ambigua collected in Klawer, Western Cape of South Africa, in ...
Ardley, Julie, Bruce, David, Chen, I-Min, De Meyer, Sofie, Detter, Chris, Goodwin, Lynne, Han, Cliff, Han, James, Howieson, John, Huntemann, Marcel, Ivanova, Natalia, Kyrpides, Nikos, Lu, Megan, Markowitz, Victor, Mavromatis, Konstantinos, Melino, Vanesssa, Mikhailova, Natalia, O'Hara, Graham, Ovchinnikova, Galina, Pagani, Ioanna, Pati, Amrita, Peters, Lin, Pitluck, Sam, Reeve, Wayne, Rui, Tian, Szeto, Ernest, Tapia, Roxanne, Terpolilli, Jason, Tiwari, Ravi, Wei, Chia-Lin, Woyke, Tanja, Yates, Ron   +31 more
core   +1 more source