Results 61 to 70 of about 2,587,498 (385)

Preliminary Study of BAC Library Construction in Black Tiger Shrimp, Penaeus monodon

open access: yesWalailak Journal of Science and Technology, 2011
Availability of shrimp genome information is necessary for shrimp genetic studies and large-insert DNA clones, bacterial artificial chromosome (BACs) serve as valuable tools for obtaining genomic sequences.
Suwit WUTHISUTHIMETHAVEE   +3 more
doaj   +1 more source

Reducing assembly complexity of microbial genomes with single-molecule sequencing [PDF]

open access: yes, 2013
Background: The short reads output by first- and second-generation DNA sequencing instruments cannot completely reconstruct microbial chromosomes. Therefore, most genomes have been left unfinished due to the significant resources required to manually close gaps in draft assemblies.
arxiv   +1 more source

Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin

open access: yesBMC Plant Biology, 2009
BackgroundExposure to dietary wheat proteins in genetically susceptible individuals has been associated with increased risk for the development of Type 1 diabetes (T1D).
E. Loit   +4 more
semanticscholar   +1 more source

Identification and validation of microsatellite markers in strawberry tree (Arbutusunedo L.) [PDF]

open access: yes, 2019
Strawberry tree (Arbutus unedo L.), an evergreen shrub/small tree of the family Ericaceae, is a main constituent of the Mediterranean basin flora; although it is also found in southwestern Prance, Macaronesia, and Ireland. The small fruits are edible but
Carlier, Jorge   +4 more
core   +1 more source

Social context prevents heat hormetic effects against mutagens during fish development

open access: yesFEBS Letters, EarlyView.
This study shows that sublethal heat stress protects fish embryos against ultraviolet radiation, a concept known as ‘hormesis’. However, chemical stress transmission between fish embryos negates this protective effect. By providing evidence for the mechanistic molecular basis of heat stress hormesis and interindividual stress communication, this study ...
Lauric Feugere   +5 more
wiley   +1 more source

Genome Compression Against a Reference [PDF]

open access: yesarXiv, 2020
Being able to store and transmit human genome sequences is an important part in genomic research and industrial applications. The complete human genome has 3.1 billion base pairs (haploid), and storing the entire genome naively takes about 3 GB, which is infeasible for large scale usage. However, human genomes are highly redundant.
arxiv  

Microsatellite primers for red drum (Sciaenops ocellatus) [PDF]

open access: yes, 2008
In this note, we document polymerase-chain-reaction (PCR) primer pairs for 101 nuclear-encoded microsatellites designed and developed from a genomic library for red drum (Sciaenops ocellatus).
Gold, John R.   +3 more
core  

Optimizing a PCR protocol for cpn60-based microbiome profiling of samples variously contaminated with host genomic DNA. [PDF]

open access: yes, 2015
The current recommended protocol for chaperonin-60 (cpn60) universal target based microbiome profiling includes universal PCR of microbiome samples across an annealing temperature gradient to maximize the diversity of sequences amplified.
Chaban, B   +3 more
core   +1 more source

STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence

open access: yesFEBS Letters, EarlyView.
Heart failure with preserved ejection fraction (HFpEF) accounts for half of the heart failure cases. It is characterised by microvascular dysfunction, associated with reduced pericyte coverage and diminished STAT3 expression in pericytes. Loss of STAT3 impairs pericyte adhesion, promotes senescence, and activates a pro‐fibrotic gene program.
Leah Rebecca Vanicek   +15 more
wiley   +1 more source

Phage-DMS: A Comprehensive Method for Fine Mapping of Antibody Epitopes

open access: yesiScience, 2020
Summary: Understanding the antibody response is critical to developing vaccine and antibody-based therapies and has inspired the recent development of new methods to isolate antibodies.
Meghan E. Garrett   +5 more
doaj  

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