Results 61 to 70 of about 596,095 (346)
Molecular Oncology, EarlyView.The LEXOVE prospective study evaluated plasma cell‐free extracellular vesicle (cfEV) dynamics using Bradford assay and dynamic light scattering in metastatic non‐small cell lung cancer patients undergoing first‐line treatments, correlating a ∆cfEV < 20% with improved median progression‐free survival in responders versus non‐responders.
Valerio Gristina +17 more
wiley +1 more source
Targeted sequencing library preparation by genomic DNA circularization
BMC Biotechnology, 2011 Background For next generation DNA sequencing, we have developed a rapid and simple approach for preparing DNA libraries of targeted DNA content. Current protocols for preparing DNA for next-generation targeted sequencing are labor-intensive, require ...
Myllykangas Samuel +3 more
doaj +1 more source
Evolution: Life has Evolved to Evolve [PDF]
Phys. Life Rev. 10 (2013) 333-335, 2014 Jim Shapiro synthesizes a great many observations about the mechanisms of evolution to reach the remarkable conclusion that large-scale modification, exchange, and rearrangement of the genome are common and should be viewed as fundamental features of life.
arxiv +1 more source
Genomic resource development for a diploid mint: Mentha longifolia [PDF]
, 2010 This research project aimed to develop genomic resources needed to enable construction of a genetic linkage map of the diploid mint species Mentha longifolia.
Hadadian, Zahra
core +1 more source
Efficient depletion of host DNA contamination in malaria clinical sequencing. [PDF]
, 2013 The cost of whole-genome sequencing (WGS) is decreasing rapidly as next-generation sequencing technology continues to advance, and the prospect of making WGS available for public health applications is becoming a reality. So far, a number of studies have
Alcock, Daniel +11 more
core +1 more source
A binary vector for transferring genomic libraries to plants [PDF]
Nucleic Acids Research, 1986 The transformation of mutant plants with a complete recombinant library derived from wild-type DNA followed by assay of transformed plants for complementation of the mutant phenotype is a promising method for the isolation of plant genes. The small genome of Arabidopsis thaliana is a good candidate for attempting this so-called shotgun transformation ...
Simoens, Chris +8 more
openaire +4 more sources
Molecular Oncology, EarlyView.We quantified and cultured circulating tumor cells (CTCs) of 62 patients with various cancer types and generated CTC‐derived tumoroid models from two salivary gland cancer patients. Cellular liquid biopsy‐derived information enabled molecular genetic assessment of systemic disease heterogeneity and functional testing for therapy selection in both ...
Nataša Stojanović Gužvić +31 more
wiley +1 more source
Facile, High Quality Sequencing of Bacterial Genomes from Small Amounts of DNA
International Journal of Genomics, 2014 Sequencing bacterial genomes has traditionally required large amounts of genomic DNA (~1 μg). There have been few studies to determine the effects of the input DNA amount or library preparation method on the quality of sequencing data.
Momchilo Vuyisich +9 more
doaj +1 more source
Cloning and characterisation of two subtilisin-like protease genes from Neotyphodium lolii : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Molecular Genetics at Massey University [PDF]
, 2000 PCR amplification of Neotyphodium lolii genomic DNA with degenerate primers detected two different sequences with homology to subtilisin-like proteases. These two PCR products were used to screen a N. lolii Lp19 genomic library.
McGill, Michelle Kay
core
Comparing Three Approaches [PDF]
, 2014 Hybridization-based target enrichment protocols require relatively large starting amounts of genomic DNA, which is not always available. Here, we tested three approaches to pre-capture library preparation starting from 10 ng of genomic DNA: (i and ii ...
Amstislavskiy, Vyacheslav S. +5 more
core +1 more source