Results 81 to 90 of about 2,587,498 (385)
Evolution: Life has Evolved to Evolve [PDF]
Phys. Life Rev. 10 (2013) 333-335, 2014 Jim Shapiro synthesizes a great many observations about the mechanisms of evolution to reach the remarkable conclusion that large-scale modification, exchange, and rearrangement of the genome are common and should be viewed as fundamental features of life.
arxiv +1 more source
Genomic resource development for a diploid mint: Mentha longifolia [PDF]
, 2010 This research project aimed to develop genomic resources needed to enable construction of a genetic linkage map of the diploid mint species Mentha longifolia.
Hadadian, Zahra
core +1 more source
Genome-Scale Validation of Deep-Sequencing Libraries
PLoS ONE, 2008 Chromatin immunoprecipitation followed by high-throughput (HTP) sequencing (ChIP-seq) is a powerful tool to establish protein-DNA interactions genome-wide. The primary limitation of its broad application at present is the often-limited access to sequencers.
D. Schmidt† +4 more
openaire +5 more sources
Molecular Oncology, EarlyView.We quantified and cultured circulating tumor cells (CTCs) of 62 patients with various cancer types and generated CTC‐derived tumoroid models from two salivary gland cancer patients. Cellular liquid biopsy‐derived information enabled molecular genetic assessment of systemic disease heterogeneity and functional testing for therapy selection in both ...
Nataša Stojanović Gužvić +31 more
wiley +1 more source
Targeted sequencing library preparation by genomic DNA circularization
BMC Biotechnology, 2011 Background For next generation DNA sequencing, we have developed a rapid and simple approach for preparing DNA libraries of targeted DNA content. Current protocols for preparing DNA for next-generation targeted sequencing are labor-intensive, require ...
Myllykangas Samuel +3 more
doaj +1 more source
Cosmid Cloning of Five \u3cem\u3eZymomonas trp\u3c/em\u3e Genes by Complementation of \u3cem\u3eEscherichia coli\u3c/em\u3e and \u3cem\u3ePseudomonas putida trp\u3c/em\u3e Mutants [PDF]
, 1988 A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z.
Eddy, Christina Kay +2 more
core +1 more source
A Human Genomic Library Enriched in Transcriptionally Active Sequences (aDNA Library) [PDF]
Genome Research, 2000 Core histone hyperacetylation, in particular of H4, is concentrated in the promoter–upstream regions of active genes and in certain cases is locuswide. Antibodies to hyperacetylated H4 were used to immunoprecipitate dinucleosomal chromatin derived from K562 human erythroleukemic cells by micrococcal nuclease digestion. The extracted DNA was made into a
Pelling, A. +2 more
openaire +4 more sources
Molecular Oncology, EarlyView.The authors analyzed the spatial distributions of gene and metabolite profiles in cervical cancer through spatial transcriptomic and spatially resolved metabolomic techniques. Pivotal genes and metabolites within these cases were then identified and validated.
Lixiu Xu +3 more
wiley +1 more source
Generating site saturation mutagenesis libraries and transferring them to broad host range plasmids using type IIS restriction enzymes [PDF]
arXiv, 2023 Protein engineering is an established method for tailoring enzymatic reactivity. A commonly used method is directed evolution, where the mutagenesis and natural selection process is mimicked and accelerated in the laboratory. Here, we describe a reliable method for generating saturation mutagenesis libraries by golden gate cloning in a broad host range
arxiv
Rainbow Smelt (Osmerus mordax) Genomic Library and EST Resources
Marine Biotechnology, 2008 Genomic resources in rainbow smelt (Osmerus mordax) enable us to examine the genome duplication process in salmonids and test hypotheses relating to the fate of duplicated genes.
K. R. Schalburg +14 more
semanticscholar +1 more source