Reference gene validation for gene expression normalization in canine osteosarcoma: a geNorm algorithm approach [PDF]
BMC Veterinary Research, 2017 Background Quantitative PCR (qPCR) is a common method for quantifying mRNA expression. Given the heterogeneity present in tumor tissues, it is crucial to normalize target mRNA expression data using appropriate reference genes that are stably expressed ...
Gayathri Thevi Selvarajah +4 more
doaj +4 more sources
Identification of endogenous reference genes for RT-qPCR analysis in breast cancer and matched adjacent tissues [PDF]
Frontiers in OncologyBackgroundReal-time quantitative PCR (RT-qPCR), essential for gene expression and biomarker studies, requires stable endogenous reference genes (RGs) for normalization. This study aimed to identify consistently expressed RGs in breast cancer and adjacent
Yue Meng +8 more
doaj +2 more sources
The validation of Short Interspersed Nuclear Elements (SINEs) as a RT-qPCR normalization strategy in a rodent model for temporal lobe epilepsy. [PDF]
PLoS ONE, 2019 BackgroundIn gene expression studies via RT-qPCR many conclusions are inferred by using reference genes. However, it is generally known that also reference genes could be differentially expressed between various tissue types, experimental conditions and ...
René A J Crans +8 more
doaj +5 more sources
Selection of reference genes for quantitative gene expression normalization in flax (
BMC Plant Biology, 2010 Background Quantitative real-time PCR (qRT-PCR) is currently the most accurate method for detecting differential gene expression. Such an approach depends on the identification of uniformly expressed 'housekeeping genes' (HKGs).
Neutelings Godfrey +2 more
doaj +7 more sources
ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP Were Identified as Reference Genes in Neuroendocrine Lung Cancer via the nCounter Technology. [PDF]
PLoS ONE, 2016 Neuroendocrine lung cancer (NELC) represents 25% of all lung cancer cases and large patient collectives exist as formalin-fixed, paraffin-embedded (FFPE) tissue only.
Robert Fred Henry Walter +9 more
doaj +4 more sources
Effective Alu repeat based RT-qPCR normalization in cancer cell perturbation experiments [PDF]
, 2013 Background: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories.
Beckers, Anneleen +13 more
core +16 more sources
GeneChip, geNorm, and gastrointestinal tumors: novel reference genes for real-time PCR [PDF]
Physiological Genomics, 2007 Accurate quantitation of target genes depends on correct normalization. Use of genes with variable tissue transcription ( GAPDH) is problematic, particularly in clinical samples, which are derived from different tissue sources. Using a large-scale gene database (Affymetrix U133A) data set of 36 gastrointestinal (GI) tumors and normal tissues, we ...
Mark, Kidd +7 more
openaire +2 more sources
Cumhuriyet Science Journal, 2022 Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating and targeting expression of genes.
Emin Ufuk Karakaş +2 more
doaj +1 more source
Heliyon, 2023 Objective: To screen and validate reference genes suitable for gene mRNA expression study in peripheral blood mononuclear cells (PBMCs) between septic patients and healthy controls (HC).
Ruoyu Song +10 more
doaj +1 more source
Biologia Plantarum, 2020 Appropriate choice of reference genes for data normalization is of critical importance for accurate real time reverse transcription quantitative PCR (RT-qPCR) analysis of gene expression.
Z.L. DUAN, W.H. HAN, L. YAN, B. WU
doaj +1 more source