Results 11 to 20 of about 16,273 (193)

The validation of Short Interspersed Nuclear Elements (SINEs) as a RT-qPCR normalization strategy in a rodent model for temporal lobe epilepsy [PDF]

PLoS ONE, 2019
Background : In gene expression studies via RT-qPCR many conclusions are inferred by using reference genes. However, it is generally known that also reference genes could be differentially expressed between various tissue types, experimental conditions ...
Crans, René, Daelemans, Sofie, De Deyn, Peter P, Janssens, Jana, Raedt, Robrecht, Stove, Christophe, Van Craenenbroeck, Kathleen, Van Dam, Debby, Wouters, Elise   +8 more
core   +5 more sources

ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP Were Identified as Reference Genes in Neuroendocrine Lung Cancer via the nCounter Technology [PDF]

PLoS ONE, 2016
Background Neuroendocrine lung cancer (NELC) represents 25% of all lung cancer cases and large patient collectives exist as formalin-fixed, paraffin-embedded (FFPE) tissue only.
Christoph, Daniel Christian, Flom, Elena, Hager, Thomas, Vollbrecht, Claudia, Walter, Robert Fred Henry, Werner, Robert   +5 more
core   +3 more sources

Selection and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Blaptica Dubia (Serville, 1838) (Blattidae, Blaberidae)

Cumhuriyet Science Journal, 2022
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating and targeting expression of genes.
Emin Ufuk Karakaş, Şeyda Berk, Ayşe Nur Pektaş   +2 more
doaj   +1 more source

Validation of reference genes for the normalization of the RT-qPCR in peripheral blood mononuclear cells of septic patients

Heliyon, 2023
Objective: To screen and validate reference genes suitable for gene mRNA expression study in peripheral blood mononuclear cells (PBMCs) between septic patients and healthy controls (HC).
Ruoyu Song, Shijun He, Yongbin Wu, Wanxin Chen, Jie Song, Yaxi Zhu, Huan Chen, Qianlu Wang, Shouman Wang, Sichuang Tan, Sipin Tan   +10 more
doaj   +1 more source

Reference gene selections for real time quantitative PCR analysis of gene expression in different oat tissues and under salt stress

Biologia Plantarum, 2020
Appropriate choice of reference genes for data normalization is of critical importance for accurate real time reverse transcription quantitative PCR (RT-qPCR) analysis of gene expression.
Z.L. DUAN, W.H. HAN, L. YAN, B. WU
doaj   +1 more source

Transcriptome-based validation of proper reference genes for reverse trascription quantitative PCR analysis of Sinocalycanthus chinensis

Biologia Plantarum, 2020
Reverse transcription quantitative PCR is a widely used method to detect gene expressions. To obtain accurate expression results, the selection of proper reference genes is important and necessary.
C. ZHANG, Q.-N. JIANG, Y.-G. WANG, J.-X. FU, B. DONG, L.-H. ZHOU, H.-B. ZHAO   +6 more
doaj   +1 more source

Transcriptome-based screening and the optimal reference genes for real-time quantitative PCR in Rehmannia chingii and R. henryi

Biologia Plantarum, 2020
Real time quantitative PCR (qPCR) is a powerful tool for studying the expression of specific genes. The accuracy and reliability of qPCR analysis data require the selection of reference genes with stable expression.
X. ZUO, F.-Q. WANG, X.-R. LI, M.-M. LI
doaj   +1 more source

LEMming: A Linear Error Model to Normalize Parallel Quantitative Real-Time PCR (qPCR) Data as an Alternative to Reference Gene Based Methods. [PDF]

PLoS ONE, 2015
Gene expression analysis is an essential part of biological and medical investigations. Quantitative real-time PCR (qPCR) is characterized with excellent sensitivity, dynamic range, reproducibility and is still regarded to be the gold standard for ...
Ronny Feuer, Sebastian Vlaic, Janine Arlt, Oliver Sawodny, Uta Dahmen, Ulrich M Zanger, Maria Thomas   +6 more
doaj   +1 more source

Selection of reference genes for gene expression studies in Siberian Apricot (Prunus sibirica L.) Germplasm using quantitative real-time PCR. [PDF]

PLoS ONE, 2014
Quantitative real time reverse transcription polymerase chain reaction has been applied in a vast range of studies of gene expression analysis. However, real-time PCR data must be normalized with one or more reference genes.
Jun Niu, Baoqing Zhu, Jian Cai, Peixue Li, Libing Wang, Huitang Dai, Lin Qiu, Haiyan Yu, Denglong Ha, Haiyan Zhao, Zhixiang Zhang, Shanzhi Lin   +11 more
doaj   +1 more source

Identification of endogenous reference genes for RT-qPCR analysis in breast cancer and matched adjacent tissues [PDF]

Front Oncol
BackgroundReal-time quantitative PCR (RT-qPCR), essential for gene expression and biomarker studies, requires stable endogenous reference genes (RGs) for normalization. This study aimed to identify consistently expressed RGs in breast cancer and adjacent
Meng Y, Wang Y, Xu Z, Qiao Z, Liu C, Chen Y, Xu Y, Zhang K.   +7 more
europepmc   +2 more sources