Results 11 to 20 of about 16,155 (172)

Transcriptome-based validation of proper reference genes for reverse trascription quantitative PCR analysis of Sinocalycanthus chinensis

open access: yesBiologia Plantarum, 2020
Reverse transcription quantitative PCR is a widely used method to detect gene expressions. To obtain accurate expression results, the selection of proper reference genes is important and necessary.
C. ZHANG   +6 more
doaj   +1 more source

GenORM: Generalizable One-shot Rope Manipulation with Parameter-Aware Policy

open access: yes, 2023
Due to the inherent uncertainty in their deformability during motion, previous methods in rope manipulation often require hundreds of real-world demonstrations to train a manipulation policy for each rope, even for simple tasks such as rope goal reaching, which hinder their applications in our ever-changing world.
Kuroki, So   +9 more
openaire   +2 more sources

Transcriptome-based screening and the optimal reference genes for real-time quantitative PCR in Rehmannia chingii and R. henryi

open access: yesBiologia Plantarum, 2020
Real time quantitative PCR (qPCR) is a powerful tool for studying the expression of specific genes. The accuracy and reliability of qPCR analysis data require the selection of reference genes with stable expression.
X. ZUO, F.-Q. WANG, X.-R. LI, M.-M. LI
doaj   +1 more source

LEMming: A Linear Error Model to Normalize Parallel Quantitative Real-Time PCR (qPCR) Data as an Alternative to Reference Gene Based Methods. [PDF]

open access: yesPLoS ONE, 2015
Gene expression analysis is an essential part of biological and medical investigations. Quantitative real-time PCR (qPCR) is characterized with excellent sensitivity, dynamic range, reproducibility and is still regarded to be the gold standard for ...
Ronny Feuer   +6 more
doaj   +1 more source

Selection of Reference Genes for Quantitative Real-time PCR Analysis in Canine Mammary Tumors Using the GeNorm Algorithm [PDF]

open access: yesVeterinary Pathology, 2006
Eleven reference genes (18s ribosomal ribonucleic acid [RNA], 28s ribosomal RNA, ubiquitin, beta-actin, glycerine aldehyde dehydrogenase, ATP-synthase subunit 5B, hydroxymethylbilane synthase, hypoxanthine-phosphoribosyl transferase, ribosomal protein L32, tryptophan 5monooxygenase activation protein (zeta polypeptide), and TATA-Box binding protein ...
B, Etschmann   +4 more
openaire   +2 more sources

Selection of reference genes for gene expression studies in Siberian Apricot (Prunus sibirica L.) Germplasm using quantitative real-time PCR. [PDF]

open access: yesPLoS ONE, 2014
Quantitative real time reverse transcription polymerase chain reaction has been applied in a vast range of studies of gene expression analysis. However, real-time PCR data must be normalized with one or more reference genes.
Jun Niu   +11 more
doaj   +1 more source

A simple method to assess group difference in RT-qPCR reference gene selection using GeNorm: The case of the placental sex [PDF]

open access: yesPlacenta, 2017
AbstractNormalization with proper reference genes is a crucial step in obtaining accurate mRNA expression levels in RT-qPCR experiments. GeNorm and NormFinder are two commonly used software packages that help in selecting the best reference genes, based on their expression stability.
Joey St-Pierre   +2 more
openaire   +5 more sources

Selection of reference genes for transcriptional analysis of edible tubers of potato (Solanum tuberosum L.). [PDF]

open access: yesPLoS ONE, 2015
Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts.
Roberta Fogliatto Mariot   +7 more
doaj   +1 more source

Transcriptome-Based Selection and Validation of Reference Genes for Gene Expression Analysis of Alicyclobacillus acidoterrestris Under Acid Stress

open access: yesFrontiers in Microbiology, 2021
Alicyclobacillus acidoterrestris is a major concern in fruit juice industry due to its spoilage potential of acidic fruit juice. Quantifying the expression levels of functional genes by real-time quantitative polymerase chain reaction (RT-qPCR) is ...
Ning Zhao   +6 more
doaj   +1 more source

Stability analysis of reference genes for RT-qPCR assays involving compatible and incompatible Ralstonia solanacearum-tomato ‘Hawaii 7996’ interactions

open access: yesScientific Reports, 2021
Reverse transcription-quantitative PCR (RT-qPCR) is an analytical tool for gene expression quantification. Reference genes are not yet available for gene expression analysis during interactions of Ralstonia solanacearum with ‘Hawaii 7996’ (the most ...
Greecy M. R. Albuquerque   +7 more
doaj   +1 more source

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