Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus [PDF]
, 2008 Background During the last years the quantification of immune response under immunological challenges, e.g. parasitation, has been a major focus of research.
A Bas +29 more
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The Plant Pathology Journal, 2019 Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However,
Li-hua Xie +10 more
doaj +1 more source
Expression of inflammation-related genes is associated with adipose tissue location in horses [PDF]
, 2013 Background : In humans, adipose tissue (AT) originating from different depots shows varying gene expression profiles. In horses, the risk of certain metabolic disorders may also be influenced by the impact of specific AT depots.
Bruynsteen, Lien +6 more
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Identification and validation of miRNAs as endogenous controls for RQ-PCR in blood specimens for breast cancer studies. [PDF]
PLoS ONE, 2013 INTRODUCTION: A prerequisite to accurate interpretation of RQ-PCR data is robust data normalization. A commonly used method is to compare the cycle threshold (CT) of target miRNAs with those of a stably expressed endogenous (EC) miRNA(s) from the same ...
Ailbhe M McDermott +2 more
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Gene expression studies: How to obtain accurate and reliable data by quantitative real-time RT PCR [PDF]
Journal of Medical Biochemistry, 2013 Real-time RT PCR has been recognized as an accurate, reliable and sensitive method for quantifying gene transcription. However, several steps preceding PCR represent critical points and source of inaccuracies.
Nestorov Jelena +3 more
doaj
Leukocyte count affects expression of reference genes in canine whole blood samples
BMC Research Notes, 2011 Background The dog is frequently used as a model for hematologic human diseases. In this study the suitability of nine potential reference genes for quantitative RT-PCR studies in canine whole blood was investigated.
Dekker Aldo +4 more
doaj +1 more source
Stable Reference Gene Selection for RT-qPCR Analysis in Nonviruliferous and Viruliferous Frankliniella occidentalis. [PDF]
PLoS ONE, 2015 Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for measuring and evaluating gene expression during variable biological processes.
Chunxiao Yang +8 more
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Identification and evaluation of suitable reference genes for gene expression studies in the whitefly Bemisia tabaci (Asia I) by reverse transcription quantitative realtime PCR [PDF]
, 2014 This study presents a reliable method for performing reverse transcription quantitative realtime PCR (RT-qPCR) to measure gene expression in the whitefly Bemisia tabaci (Asia I) (Gennadius) (Hemiptera: Aleyrodidae), utilising suitable reference genes for
Carl Collins +6 more
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Scientific Reports, 2022 Examination of transcriptional regulation occurring during pregnancy establishment and maintenance requires the identification of endogenous reference genes characterized by high expression stability.
Magdalena Sozoniuk +3 more
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Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data [PDF]
, 2007 Background Real-time reverse transcriptase quantitative polymerase chain reaction (real-time RT-qPCR) is the most accurate measure of gene expression in biological systems. The comparison of different samples requires the transformation of data through a
Clements, Dylan N. +5 more
core +4 more sources