Prawn Shell Chitosan Has Anti-Obesogenic Properties, Influencing Both Nutrient Digestibility and Microbial Populations in a Pig Model [PDF]
, 2015 This study was supported financially (Grant-Aid Agreement No. MFFRI/07/01) under the Sea Change Strategy with the support of the Marine Institute and the Department of Agriculture, Food and the Marine, funded under the National Development Plan 2007 ...
Egan, Aine M. +3 more
core +1 more source
The Plant Pathology Journal, 2019 Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However,
Li-hua Xie +10 more
doaj +1 more source
Identification and validation of miRNAs as endogenous controls for RQ-PCR in blood specimens for breast cancer studies. [PDF]
PLoS ONE, 2013 INTRODUCTION: A prerequisite to accurate interpretation of RQ-PCR data is robust data normalization. A commonly used method is to compare the cycle threshold (CT) of target miRNAs with those of a stably expressed endogenous (EC) miRNA(s) from the same ...
Ailbhe M McDermott +2 more
doaj +1 more source
Expression of inflammation-related genes is associated with adipose tissue location in horses [PDF]
, 2013 Background : In humans, adipose tissue (AT) originating from different depots shows varying gene expression profiles. In horses, the risk of certain metabolic disorders may also be influenced by the impact of specific AT depots.
Bruynsteen, Lien +6 more
core +2 more sources
Gene expression studies: How to obtain accurate and reliable data by quantitative real-time RT PCR [PDF]
Journal of Medical Biochemistry, 2013 Real-time RT PCR has been recognized as an accurate, reliable and sensitive method for quantifying gene transcription. However, several steps preceding PCR represent critical points and source of inaccuracies.
Nestorov Jelena +3 more
doaj
Leukocyte count affects expression of reference genes in canine whole blood samples
BMC Research Notes, 2011 Background The dog is frequently used as a model for hematologic human diseases. In this study the suitability of nine potential reference genes for quantitative RT-PCR studies in canine whole blood was investigated.
Dekker Aldo +4 more
doaj +1 more source
Altered expression of neuroplasticity-related genes in the brain of depressed suicides [PDF]
, 2015 Background: Expression of the neuronal membrane glycoprotein M6a (GPM6A), the proteolipid protein (PLP/DM20) family member, is downregulated in the hippocampus of chronically stressed animals.
Alvarez Juliá, Anabel +4 more
core +1 more source
Diel pattern of circadian clock and storage protein gene expression in leaves and during seed filling in cowpea (Vigna unguiculata) [PDF]
, 2018 Background Cowpea (Vigna unguiculata) is an important source of protein supply for animal and human nutrition. The major storage globulins VICILIN and LEGUMIN (LEG) are synthesized from several genes including LEGA, LEGB, LEGJ and CVC (CONVICILIN).
Egea Gutiérrez-Cortines, Marcos +6 more
core +4 more sources
Stable Reference Gene Selection for RT-qPCR Analysis in Nonviruliferous and Viruliferous Frankliniella occidentalis. [PDF]
PLoS ONE, 2015 Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for measuring and evaluating gene expression during variable biological processes.
Chunxiao Yang +8 more
doaj +1 more source
Scientific Reports, 2022 Examination of transcriptional regulation occurring during pregnancy establishment and maintenance requires the identification of endogenous reference genes characterized by high expression stability.
Magdalena Sozoniuk +3 more
doaj +1 more source