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The Use of Immunological Techniques to Detect Cells of Rare Genotype

1982
An increase in the mutation rate in germinal cells may result in an increase in the number of genetically defective persons in the next and future generations, and mutations in somatic cells may be the possible cause of such diseases as cancer(1–3) and of advanced aging.(4) These health effects result in decreased fitness and life-span of and emotional
Aftab A. Ansari, H. V. Mailing
openaire   +2 more sources

Improvement on PCR-CTPP: a SNP genotyping approach based on mismatch technique

Hereditas (Beijing), 2011
To explore the technique principle of PCR with confronting two-pair primers (PCR-CTPP) and improve the accuracy of SNP genotyping by taking the 1298 locus of human gene MTHFR as an example, the reliability between conventional PCR-CTPP and improved PCR-CTPP was compared using reconstructed PCR-CTPP detecting system in terms of designing appropriate ...
Yu-Xia Yun   +8 more
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Evaluation of fingerprinting techniques to assess genotype variation among Zygosaccharomyces strains

Food Microbiology, 2018
Molecular typing techniques are key tools in surveillance of food spoilage yeasts, in investigations on intra-species population diversity, and in tracing selected starters during fermentation. Unlike previous works on strain typing of Zygosaccharomyces spoilage species, here Zygosaccharomyces mellis and the Zygosaccharoymces rouxii complex yeasts ...
Dakal TC, Solieri L, Giudici P.
openaire   +4 more sources

Semi-micro techniques for the genotyping of galactokinase and galactose-1-phosphate uridyltransferase

Clinica Chimica Acta, 1982
Abstract Semi-micro techniques are presented for the genotyping of galactokinase and galactose-1-phosphate uridyltransferase. Radioactive assays are used to quantitate the levels of enzyme activity, while agarose gel electrophoresis is used to type the variants of galactose-1-phosphate uridyltransferase.
Jo Ellen Sheets Lee, Won G. Ng
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PCR-CTPP: a new genotyping technique in the era of genetic epidemiology

Expert Review of Molecular Diagnostics, 2001
A new PCR method, PCR-CTPP (polymerase chain reaction with confronting two-pair primers) was invented to genotype a relatively large number of samples in a cost-effective and time-saving manner. In this method, allele-specific DNA products are amplified by means of applying appropriately designed two-pair primers (four primers) into an ordinary PCR ...
openaire   +2 more sources

Morphology Antibody Chromosome Technique for Determining Phenotype and Genotype of the Same Cell

Current Protocols in Human Genetics, 1996
AbstractThe morphology antibody chromosome (MAC) technique is a powerful combination of methods that permits analysis of both phenotype and genotype on a single interphase or mitotic cell as a basis for lineage analysis of neoplastic and normal cells. This unit describes MAC with sequential phenotypic analysis using antibody and an alkaline phosphatase
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Molecular Techniques for Genotyping

2020
Genotyping is a process of determining the genetic constituent/genetic makeup “genotype” of an organism by examining the individual DNA sequence and comparing to a reference or other individual sequence. It helps the researchers to explore the genetic constitution, genetic linkages or variations like Single Nucleotide Polymorphisms (SNP) or multi ...
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GENOTYPE RESPONSE TO IN VITRO GYNOGENESIS TECHNIQUE IN GERBERA JAMESONII

Acta Horticulturae, 1990
A. Tosca   +4 more
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