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Methods for Expressing and Analyzing GFP-Tubulin and GFP-Microtubule-Associated Proteins
Cold Spring Harbor Protocols, 2010INTRODUCTIONImportant advances in our understanding of the organization and dynamics of the cytoskeleton have been made by direct observations of fluorescently tagged cytoskeletal proteins in living cells. In early experiments, the cytoskeletal protein of interest was purified, covalently modified with a fluorescent dye, and microinjected into living ...
Holly V, Goodson +2 more
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Chemical & Engineering News Archive, 2009
CHEMISTS HAVE USED ultrafast Raman spectroscopy to map the structural evolution of the green fluorescent protein (GFP) chromophore during proton transfer. The findings not only help explain the workings of GFP but could also lead to a better fundamental understanding of proton transfer, which drives key biological processes. “Since the discovery in our
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CHEMISTS HAVE USED ultrafast Raman spectroscopy to map the structural evolution of the green fluorescent protein (GFP) chromophore during proton transfer. The findings not only help explain the workings of GFP but could also lead to a better fundamental understanding of proton transfer, which drives key biological processes. “Since the discovery in our
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GFP as potential cellular viscosimeter
Methods and Applications in Fluorescence, 2016The molecular dimensions of proteins such as green fluorescent protein (GFP) are large as compared to the ones of solvents like water or glycerol. The microscopic viscosity, which determines the resistance to diffusion of, e.g. GFP, is then the same as that determined from the resistance of the solvent to flow, which is known as macroscopic viscosity ...
Visser, Antonie +3 more
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2011
?????????????????????????????? ???????????????????????????? ???????????????????????????? ?????????????? Ac-CMV-GFP, Ac-M-GFP ?? Ac-IFN-GFP ?? ???????????????????? ?????????????????????????? ???????????????????? (???????????????? CMV ?? ?????????????? CAG), ???????????????????????? ???????????????????? ???????????????????????? ???????? eGfp ?? ???????? ?
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?????????????????????????????? ???????????????????????????? ???????????????????????????? ?????????????? Ac-CMV-GFP, Ac-M-GFP ?? Ac-IFN-GFP ?? ???????????????????? ?????????????????????????? ???????????????????? (???????????????? CMV ?? ?????????????? CAG), ???????????????????????? ???????????????????? ???????????????????????? ???????? eGfp ?? ???????? ?
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Trends in Genetics, 1995
Green fluorescent protein (GFP) is responsible for the green bioluminescence from the jellyfish Aequorea victoria. The intense fluorescence of GFP is due to the nature of a chromophore composed of modified amino acids within the polypeptide. Formation of the fluorescent chromophore is species independent and apparently does not require any additional ...
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Green fluorescent protein (GFP) is responsible for the green bioluminescence from the jellyfish Aequorea victoria. The intense fluorescence of GFP is due to the nature of a chromophore composed of modified amino acids within the polypeptide. Formation of the fluorescent chromophore is species independent and apparently does not require any additional ...
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2021
The results of Agrobacterium-mediated transformation of different flax cultivars by plasmid carrying chimeric GFP-TUA6 gene are presented in this study. Transgenic nature of obtained lines was confirmed by PCR analysis. It was shown that GFP labeled tubulin is capable to co-polymerize with endogenous cell tubulin and to participate in cortical ...
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The results of Agrobacterium-mediated transformation of different flax cultivars by plasmid carrying chimeric GFP-TUA6 gene are presented in this study. Transgenic nature of obtained lines was confirmed by PCR analysis. It was shown that GFP labeled tubulin is capable to co-polymerize with endogenous cell tubulin and to participate in cortical ...
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The green fluorescent protein (GFP) has enabled researchers to visualize a wide range of cellular processes, from protein expression to metastasis. However, its size (27 kDa) can disrupt localization and association of GFP-tagged proteins. Here, we aim to address this limitation by designing a miniature GFP (<20 kDa) that conserves its chromophore ...
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