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GFP moves on

Trends in Cell Biology, 2001
Accompanying this issue of Trends in Cell Biology is GFP in Motion 2, a second compilation on CD-ROM of video clips in which cell biology's wonder-molecule – green-fluorescent protein – displays its skills, making visible the hitherto hidden dynamics of proteins and organelles inside living cells.
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GFP illuminates the cytoskeleton

Trends in Cell Biology, 1998
Until recently, cytoskeleton research has relied primarily on immunofluorescence microscopy techniques, requiring fixation and hence killing of the specimen before the analysis. The sole method for visualizing cytoskeletal dynamics in living cells has been the microinjection of purified and fluorescently labelled protein, but technical difficulties ...
B, Ludin, A, Matus
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A polymerizable GFP variant

Protein Engineering Design and Selection, 2012
Flagellin has the ability to polymerize into long filaments under appropriate conditions. Our work aims at the construction of flagellin-based fusion proteins which possess polymerization ability and preserve the functional properties of the fusion partner as well.
Agnes, Klein   +4 more
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Targeting GFP to organelles.

Methods in cell biology, 1999
abstract not ...
DE GIORGI F   +9 more
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DNA makes GFP shine

Nature Methods, 2005
An oligomerization-dependent system comprising two fragments of green fluorescent protein (GFP) fused to engineered zinc fingers could be a means to detect virtually any double-stranded DNA sequence.
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GFP in mammalian cells

Trends in Genetics, 1995
W e are using the green fluorescent protein (GFP) to follow the behaviour of proteins in living mammalian tissue-culture cells. In particular, we are studying the localization of human cyclins, proteins that bind and activate the cyclin-dependent kinases (CDKs) that are essential to the regulation of the cell cycle.
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GFP as potential cellular viscosimeter

Methods and Applications in Fluorescence, 2016
The molecular dimensions of proteins such as green fluorescent protein (GFP) are large as compared to the ones of solvents like water or glycerol. The microscopic viscosity, which determines the resistance to diffusion of, e.g. GFP, is then the same as that determined from the resistance of the solvent to flow, which is known as macroscopic viscosity ...
Visser, Antonie   +3 more
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