Results 191 to 200 of about 465,440 (235)

A novel technique for preparation of monodisperse giant liposomes

Chemical Communications, 2003
A novel technique for the preparation of monodisperse giant liposomes has been developed based on a combination of micro-patterning of ITO glass slides with lipid solution and electroformation. The average diameter of the produced liposomes is determined by size of the micro-pattern features.
Pietro, Taylor, Chun, Xu, Paul D I, Fletcher, Vesselin N, Paunov   +3 more
openaire   +2 more sources

Spontaneous Formation of Giant Liposomes from Neutral Phospholipids

Chemistry Letters, 1997
Abstract Giant liposomes were formed spontaneously from neutral phospholipids in an aqueous solution containing MgCl2. Based on both dark-field microscopy and transmission electron microscopy, we concluded that these giant liposomes are unilamellar.
Nobuyuki Magome, Tatsunobu Takemura, Kenichi Yoshikawa   +2 more
openaire   +1 more source

Coating of POPC Giant Liposomes with Hydrophobized Polysaccharide

Chemistry Letters, 1998
Abstract POPC giant liposomes were incubated with pullulan that was hydrophobized by cholesteryl groups and labelled by a fluorescent marker (FITC). Confocal laser microscopy clearly showed coating of the outer surface of the liposomes with the polymer.
Takehiko Ueda, Stephen J Lee, Yoichi Nakatani, Guy Ourisson, Junzo Sunamoto   +4 more
openaire   +1 more source

Uniformly-sized giant liposome formation with gentle hydration

2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, 2011
This paper describes a methodology that realizes uniformly-sized liposome arrays with gentle hydration. The system consists of a dried lipid pattern on a substrate that is a micropatterned polymer thin film on an indium-tin-oxide (ITO) glass slide. An electrospray deposition (ESD) method was applied to obtain the lipid patterning at the polymer pattern
Toshihisa Osaki, Kaori Kuribayashi-Shigetomi, Ryuji Kawano, Hirotaka Sasaki, Shoji Takeuchi   +4 more
openaire   +1 more source

Spreading of giant liposomes on anisotropically patterned substrates

Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2013
The dynamic spreading of giant liposomes on anisotropically patterned substrates is investigated experimentally on vertically fixed spreading substrates. The giant liposomes are prepared through electroformation method. The substrates with anisotropic grooves on the surfaces are fabricated on silicon wafers by photolithography and dry etching.
Ying Zhang, Yan Chen, Hui Fan
openaire   +1 more source

Reconstruction of motile actin networks in giant liposome

2010 International Symposium on Micro-NanoMechatronics and Human Science, 2010
To construct a simple model cellular system exhibiting the property of self-propelled motion, cell-sized giant liposomes encapsulating desired amounts of actoHMM, a mixture of actin filament (F-actin) and heavy meromyosin (HMM, an actin-related molecular motor), have been prepared.
Kingo Takiguchi, Makiko Negishi, Yohko Tanaka-Takiguchi, Michio Homma, Kenichi Yoshikawa   +4 more
openaire   +1 more source

Transmembrane transport of multicomponent liposome-nanoparticles into giant vesicles

Chinese Physics B, 2022
With the emergence and rapid development of nanotechnology, the nanoparticles hybridized with multicomponent lipids are more and more used in gene delivery. These vectors interact with the cell membrane before entering into the cell. Therefore, the nature of this interaction is important in investigating multicomponent liposome-nanoparticle (MLP ...
Hui-Fang Wang, Chun-Rong Li, Min-Na Sun, Jun-Xing Pan, Jin-Jun Zhang   +4 more
openaire   +1 more source

Rapid Access to Giant Unilamellar Liposomes with Upper Size Control: Membrane-Gated, Gel-Assisted Lipid Hydration.

Langmuir, 2020
Combining gel-assisted lipid hydration with membrane-based lipid extrusion, we demonstrate here a general procedure for rapid preparation of giant unilamellar liposomes with upper size control. Featured in this procedure are planar lipid stacks deposited
Zening Liu, Jinyan Cui, Wei Zhan
semanticscholar   +1 more source

Novel method to form giant liposome entrapping DNA

Macromolecular Symposia, 1996
AbstractWe report a new method to prepare giant liposome with the size of several tens micrometers, entrapping DNA molecule within the closed phospholipid membrane. The formation of such liposomes has been confirmed through the observation with fluorescence microscopy.
Noriyuki Kumazawa, Yulia S. Mel'Nikova, Kenichi Yoshikawa   +2 more
openaire   +1 more source

Location of Magnetic and Fluorescent Nanoparticles Encapsulated inside Giant Liposomes

The Journal of Physical Chemistry B, 2008
In this paper, we demonstrate the production of highly magnetic and fluorescent giant vesicles by encapsulating gamma-Fe2O3-rhodamine B nanoparticles. The liposomes containing the nanoparticles were made of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC).
Grégory, Beaune, Christine, Ménager, Valérie, Cabuil   +2 more
openaire   +2 more sources