Results 71 to 80 of about 19,800 (242)

Dynamics of shape fluctuations of quasi-spherical vesicles revisited

, 2002
In this paper, the dynamics of spontaneous shape fluctuations of a single, giant quasi-spherical vesicle formed of a single lipid species is revisited theoretically.
Kleis, Jesper, Lomholt, Michael A., Miao, Ling   +2 more
core   +2 more sources

TBK1 Induces the Formation of Optineurin Filaments That Condensate with Polyubiquitin and LC3 for Cargo Sequestration

Advanced Science, EarlyView.
Phosphorylation of Optineurin by TBK1 induces the formation of filaments that condensate upon binding to linear polyubiquitin. Membrane‐anchored LC3 partitions into these condensates, suggesting that phase separation of filamentous Optineurin with ubiquitylated cargo promotes the sequestration of cargo and its subsequent alignment with LC3‐positive ...
Maria G. Herrera, Lena Kühn, Lisa Jungbluth, Verian Bader, Laura J. Krause, David Kartte, Elias Adriaenssens, Sascha Martens, Jörg Tatzelt, Carsten Sachse, Konstanze F. Winklhofer   +10 more
wiley   +1 more source

Reconstitution of proteins on electroformed giant unilamellar vesicles [PDF]

, 2015
In vitro reconstitution of simplified biological systems from molecular parts has proven to be a powerful method for investigating the biochemical and biophysical principles underlying cellular processes. In recent years, there has been a growing interest in reconstitution of protein-membrane interactions to understand the critical role played by ...
Schmid, E., Richmond, D., Fletcher, D.
openaire   +3 more sources

Lipid Lateral Organization on Giant Unilamellar Vesicles Containing Lipopolysaccharides [PDF]

Biophysical Journal, 2011
We developed a new (to our knowledge) protocol to generate giant unilamellar vesicles (GUVs) composed of mixtures of single lipopolysaccharide (LPS) species and Escherichia coli polar lipid extracts. Four different LPSs that differed in the size of the polar headgroup (i.e., LPS smooth > LPS-Ra > LPS-Rc > LPS-Rd) were selected to generate GUVs composed
Kubiak, Jakub, Brewer, Jonathan R., Hansen, Søren, Bagatolli, Luis   +3 more
openaire   +4 more sources

Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels [PDF]

BIO-PROTOCOL, 2020
Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics. While several protocols have been developed for their generation in recent years, the use of these techniques involving charged lipid types and buffers of physiological ionic ...
Parigoris, E., Dunkelmann, D. ; https://orcid.org/0000-0001-6447-7041, Silvan, U.   +2 more
openaire   +4 more sources

QS21-Initiated Fusion of Liposomal Small Unilamellar Vesicles to Form ALFQ Results in Concentration of Most of the Monophosphoryl Lipid A, QS21, and Cholesterol in Giant Unilamellar Vesicles

Pharmaceutics, 2023
Army Liposome Formulation with QS21 (ALFQ), a vaccine adjuvant preparation, comprises liposomes containing saturated phospholipids, with 55 mol% cholesterol relative to the phospholipids, and two adjuvants, monophosphoryl lipid A (MPLA) and QS21 saponin.
Erwin G. Abucayon, Mangala Rao, Gary R. Matyas, Carl R. Alving   +3 more
doaj   +1 more source

Identification and Characterization of an In Silico Designed Membrane‐Active Peptide with Antiviral Properties

Advanced Science, EarlyView.
An evolutionary molecular dynamics platform is used to design P1.6, a membrane‐active peptide that senses lipid packing defects in viral envelopes. P1.6 adopts a stabilized α‐helical structure upon membrane contact, disrupts virus‐like liposomes, and damages HIV‐1 particles.
Pascal von Maltitz, Niek van Hilten, Tatjana Weil, Thunchanok Thummaraj, Jeroen Methorst, Dennis Aschmann, Alexander Kros, Clarissa Read, Jasmina Gačanin, Herre Jelger Risselada, Jan Münch   +10 more
wiley   +1 more source

Block Copolymer Giant Unilamellar Vesicles for High-Throughput Screening

CHIMIA, 2022
Bottom-up synthetic cells offer the potential to study cellular processes with reduced complexity. Giant unilamellar vesicles (GUVs) can mimic cells in their morphological characteristics because their architecture is precisely controllable. We propose a block copolymer-based GUV system that can be used for high-throughput screening. Through droplet
Lukas Heuberger, Cornelia Palivan
openaire   +5 more sources

Wireless Bioelectronic Modulation of Membrane Potential in Glioblastoma Using Carbon Nanotube Porins

Advanced Science, EarlyView.
A schematic illustrating glioblastoma cell membrane modulation using carbon nanotube porins (CNTPs) and an applied voltage. CNTPs enable ion flux leading to changes in membrane potential. This figure summarizes the concept of bioelectronic control of cell membrane voltage.
Fleur Groualle, David Onion, Julie A. Watts, Graham A. Rance, Aleksandr Noy, Beth Coyle, Frankie J. Rawson   +6 more
wiley   +1 more source

The autophagy adaptor NDP52 and the FIP200 coiled-coil allosterically activate ULK1 complex membrane recruitment

eLife, 2020
The selective autophagy pathways of xenophagy and mitophagy are initiated when the adaptor NDP52 recruits the ULK1 complex to autophagic cargo. Hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) was used to map the membrane and NDP52 ...
Xiaoshan Shi, Chunmei Chang, Adam L Yokom, Liv E Jensen, James H Hurley   +4 more
doaj   +1 more source